Nejvíce citovaný článek - PubMed ID 1839365
Continuous-flow synthesis of alpha-gliadin peptides in an ultrasonic field and assay of their inhibition of intestinal sucrase activity
The aim of our work was to investigate the in vitro reactivity of gliadin peptides of natural and synthetic origin with various cell lines. We have found that all tested cell lines of human, mouse and rat origin were agglutinated by enzymically digested gliadin (peptic-tryptic- and peptic-tryptic pancreatic digest of alpha-gliadin) in a concentration dependent manner. In order to test the specificity of binding, inhibition studies were performed using a panel of sugars as well as natural and synthetic peptides derived from gliadin. We have found that among twelve tested sugars only fetuin and phosphomannan were able to inhibit the agglutination of K562 cells with peptic-tryptic- but not with peptic-tryptic pancreatic digest of alpha-gliadin. The lack of inhibition by gliadin peptides and most of the saccharides suggests that agglutinating activity of gliadin is the result of a nonspecific binding of gliadin to the cell membrane.
- MeSH
- adenokarcinom patologie MeSH
- akutní bazofilní leukemie patologie MeSH
- alfa-fetoproteiny farmakologie MeSH
- buněčná adheze MeSH
- Burkittův lymfom patologie MeSH
- chronická myeloidní leukemie patologie MeSH
- gliadin metabolismus MeSH
- glykoproteiny membrány trombocytů * MeSH
- kmenové buňky embryonálního karcinomu MeSH
- krysa rodu Rattus MeSH
- L buňky (buněčná linie) MeSH
- lektiny MeSH
- lidé MeSH
- lymfocyty metabolismus MeSH
- lymfom patologie MeSH
- makrofágy metabolismus MeSH
- mannany farmakologie MeSH
- molekulární sekvence - údaje MeSH
- myši MeSH
- nádorové buňky kultivované MeSH
- nádorové kmenové buňky metabolismus MeSH
- nádory maxily patologie MeSH
- nádory tračníku patologie MeSH
- peptidy farmakologie MeSH
- receptory buněčného povrchu metabolismus MeSH
- sacharidy farmakologie MeSH
- sekvence aminokyselin MeSH
- teratokarcinom patologie MeSH
- trombocytový glykoproteinový komplex Ib-IX * MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alfa-fetoproteiny MeSH
- gliadin MeSH
- glycoprotein receptor GPIb-IX MeSH Prohlížeč
- glykoproteiny membrány trombocytů * MeSH
- lektiny MeSH
- mannany MeSH
- peptidy MeSH
- phosphomannan MeSH Prohlížeč
- receptory buněčného povrchu MeSH
- sacharidy MeSH
- trombocytový glykoproteinový komplex Ib-IX * MeSH
