The quality of oil is highly dependent on its free fatty acid (FFA) content, especially due to increased restrictions on renewable fuels. As a result, there has been a growing interest in free fatty acid determination methods over the last few decades. While various standard methods are currently available, such as the American Oil Chemists Society (AOCS), International Union of Pure and Applied Chemistry (IUPAC), and Japan Oil Chemists' Society (JOCS), to obtain accurate results, there is a pressing need to investigate a fast, accurate, feasible, and eco-friendly methodology for determining FFA in biological materials. This is owing to inadequate characteristics of the methods, such as solvent consumption and reproducibility, among others. This study aims to investigate FFA determination methods to identify suitable approaches and introduce a fresh perspective.

• Klíčová slova
• FFA analysis, chromatographic methods introduction, colorimetric methods, electrochemical methods, spectroscopic methods,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

In this study a device for automatic electrochemical analysis was designed. A three electrodes detection system was attached to a positioning device, which enabled us to move the electrode system from one well to another of a microtitre plate. Disposable carbon tip electrodes were used for Cd(II), Cu(II) and Pb(II) ion quantification, while Zn(II) did not give signal in this electrode configuration. In order to detect all mentioned heavy metals simultaneously, thin-film mercury electrodes (TFME) were fabricated by electrodeposition of mercury on the surface of carbon tips. In comparison with bare electrodes the TMFEs had lower detection limits and better sensitivity. In addition to pure aqueous heavy metal solutions, the assay was also performed on mineralized rock samples, artificial blood plasma samples and samples of chicken embryo organs treated with cadmium. An artificial neural network was created to evaluate the concentrations of the mentioned heavy metals correctly in mixture samples and an excellent fit was observed (R2 = 0.9933).

• MeSH
• automatizace MeSH
• elektrochemie metody   MeSH
• elektrody MeSH
• geologické sedimenty chemie   MeSH
• ionty MeSH
• kadmium krev   MeSH
• kalibrace MeSH
• kur domácí MeSH
• lidé MeSH
• měď krev   MeSH
• neuronové sítě * MeSH
• olovo krev   MeSH
• regresní analýza MeSH
• robotika MeSH
• rtuť chemie   MeSH
• těžké kovy analýza   krev   MeSH
• zinek krev   MeSH
• životní prostředí * MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• ionty MeSH
• kadmium MeSH
• měď MeSH
• olovo MeSH
• rtuť MeSH
• těžké kovy MeSH
• zinek MeSH