Nejvíce citovaný článek - PubMed ID 33475698
A genome-wide association study reveals cytokinin as a major component in the root defense responses against Ralstonia solanacearum
To identify novel genes engaged in plant epidermal development, we characterized the phenotypic variability of rosette leaf epidermis of 310 sequenced Arabidopsis thaliana accessions, focusing on trichome shape and distribution, compositional characteristics of the trichome cell wall, and histologically detectable metal ion distribution. Some of these traits correlated with cLimate parameters of our accession's locations of origin, suggesting environmental selection. A novel metal deposition pattern in stomatal guard cells was observed in some accessions. Subsequent GWAS analysis identified 1546 loci with protein sequence-altering SNPs associated with one or more traits, including 5 genes with previously reported relevant mutant phenotypes and 80 additional genes with known or predicted roles in relevant developmental and cellular processes. Some candidates, including GFS9/TT9, exhibited environmentally correlated allele distribution. Several large gene famiLies, namely DUF674, DUF784, DUF1262, DUF1985, DUF3741, cytochrome P450, receptor-Like kinases, Cys/His-rich C1 domain proteins and formins were overrepresented among the candidates for various traits, suggesting epidermal development-related functions. A possible participation of formins in guard cell metal deposition was supported by observations in available loss of function mutants. Screening of candidate gene lists against the STRING interactome database uncovered several predominantly nuclear protein interaction networks with possible novel roles in epidermal development.
- Klíčová slova
- Arabidopsis thaliana, BioClim, GWAS, guard cell, metal accumulation, phenotypic variability, trichome,
- MeSH
- Arabidopsis * genetika metabolismus růst a vývoj MeSH
- celogenomová asociační studie * MeSH
- epidermis rostlin * metabolismus genetika růst a vývoj MeSH
- fenotyp MeSH
- jednonukleotidový polymorfismus genetika MeSH
- kovy * metabolismus MeSH
- listy rostlin * genetika metabolismus růst a vývoj MeSH
- proteiny huseníčku genetika metabolismus MeSH
- regulace genové exprese u rostlin MeSH
- rostlinné geny * MeSH
- trichomy * růst a vývoj genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kovy * MeSH
- proteiny huseníčku MeSH
