Regenerating skin tissue remains a major challenge in medical science, especially due to the risk of scarring and prolonged healing, which becomes even more complicated in people with diabetes. Recent advancements have led to the creation of therapeutic dressings incorporating drug-delivery systems to tackle these issues. Exosomes (Exos) derived from mesenchymal stem cells (MSCs) have gained significant attention for mediating therapy without directly using cells, thanks to their natural anti-inflammatory and tissue repair properties mirroring those of MSCs. In this study, an advanced wound dressing combines chitosan (CS) and polyethylene glycol (PEG) hydrogel with adipose MSCs-derived Exos (ADMSCs-Exos). This composite, formed using a straightforward blending technique, is engineered to improve the healing process of severe skin injuries by steadily releasing Exos as the hydrogel degrades. The in vitro studies demonstrate that this hydrogel-exosome dressing greatly enhances endothelial cell migration, reduces oxidative stress, and promotes angiogenesis, crucial for effective wound healing. Additionally, real time-polymerase chain reaction (RT-PCR) analysis revealed significant upregulation of key genes involved in these processes, supporting the therapeutic potential of the hydrogel-Exo combination. These findings emphasize the potential of this hydrogel-Exos combination as an innovative and promising solution for advanced wound care.

• Keywords
• angiogenesis, cell migration, chitosan‐polyethylene glycol hydrogel, mesenchymal stem cells derived exosomes, wound healing,
• MeSH
• Chitosan * chemistry   pharmacology   MeSH
• Human Umbilical Vein Endothelial Cells metabolism   MeSH
• Exosomes * metabolism   chemistry   MeSH
• Wound Healing * drug effects   MeSH
• Hydrogels * chemistry   pharmacology   MeSH
• Humans MeSH
• Mesenchymal Stem Cells * metabolism   cytology   MeSH
• Cell Movement drug effects   MeSH
• Polyethylene Glycols * chemistry   pharmacology   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Chitosan * MeSH
• Hydrogels * MeSH
• Polyethylene Glycols * MeSH