Epidemiological studies have revealed that high consumption of soy products is associated with low incidences of hormone-dependent cancers, including breast and prostate cancer. Soybeans contain large amounts of isoflavones, such as the genistein and daidzain. Previously, it has been demonstrated that genistein, one of the predominant soy isoflavones, can inhibit several steps involved in carcinogenesis. It is suggested that genistein possesses pleiotropic molecular mechanisms of action including inhibition of tyrosine kinases, DNA topoisomerase II, 5α-reductase, galectin-induced G2/M arrest, protein histidine kinase, and cyclin-dependent kinases, modulation of different signaling pathways associated with the growth of cancer cells (e.g., NF-κB, Akt, MAPK), etc. Moreover, genistein is also a potent inhibitor of angiogenesis. Uncontrolled angiogenesis is considered as a key step in cancer growth, invasion, and metastasis. Genistein was found to inhibit angiogenesis through regulation of multiple pathways, such as regulation of VEGF, MMPs, EGFR expressions and NF-κB, PI3-K/Akt, ERK1/2 signaling pathways, thereby causing strong antiangiogenic effects. This review focuses on the antiangiogenic properties of soy isoflavonoids and examines their possible underlying mechanisms.
- Klíčová slova
- angiogenesis, breast cancer, galectins, genistein, soy,
- MeSH
- genistein chemie farmakologie terapeutické užití MeSH
- Glycine max chemie MeSH
- inhibitory angiogeneze chemie farmakologie terapeutické užití MeSH
- isoflavony chemie farmakologie terapeutické užití MeSH
- lidé MeSH
- nádory prsu krevní zásobení farmakoterapie metabolismus patologie MeSH
- patologická angiogeneze farmakoterapie metabolismus patologie MeSH
- prsy krevní zásobení účinky léků metabolismus patologie MeSH
- signální transdukce účinky léků MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Názvy látek
- genistein MeSH
- inhibitory angiogeneze MeSH
- isoflavony MeSH
BACKGROUND: Cardiovascular disease is a major health issue worldwide, which has been well treated by the extracts of traditional herbs. Radix Puerariae, the dried root of the leguminous plant Pueraria lobata (Willd.) Ohwi, is a delicious vegetable in some southern provinces of China. Puerarin has also been widely used to treat human diseases, but few controlled studies are available. AIM: To determine the anti-atherosclerotic effects of puerarin on fat diet-induced atherosclerosis (AS) in rabbits. METHODS: An AS model was established by feeding 60 rabbits a high-fat diet and randomly dividing them into 6 groups: (1) normal control, (2) a model group (3) the statin, simvastatin and groups (4), (5) and (6) received 3 different amounts of puerarin. The fasting sera of all animals were collected before and after 90 days treatment to determine the levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C) and high density lipoprotein-cholesterol (HDL-C). The aortas were pathomorphologically examined. PCNA and PDGF-A protein levels were assessed by Western blot. RESULTS: On the 90th day, the levels of TC, TG and LDL-C were significantly lower in high- and middle-dose puerarin groups and simvastatin group than in the model group (P<0.05), and the HDL-C level was higher. The percentages of plaque area to the total aortic area differed significantly for high- and middle-dose puerarin groups, simvastatin group and model group (P<0.05). Whole blood viscosity increased in the high-fat diet groups, while those in the treatment groups (except for the low-dose puerarin group) were significantly lower than those in the model group (P<0.05). PCNA and PDGF-A protein expression levels of rabbit aorta were low in the normal group. Protein expression levels in the groups fed the high-fat diet were significantly increased (P<0.05), but those in the high-dose puerarin group and simvastatin group were significantly decreased compared with the model group (P<0.05). CONCLUSIONS: Puerarin inhibits the formation and development of AS plaque and suppresses the migration and reproduction of vascular smooth muscle cells by decreasing PCNA and PDGF-A expressions in the rabbit. This is encouraging in terms of cardiovascular disease prevention/treatment.
- MeSH
- ateroskleróza krev farmakoterapie MeSH
- isoflavony terapeutické užití MeSH
- králíci MeSH
- lipidy krev MeSH
- modely nemocí na zvířatech MeSH
- vazodilatancia terapeutické užití MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- isoflavony MeSH
- lipidy MeSH
- puerarin MeSH Prohlížeč
- vazodilatancia MeSH
Isoflavones are a subgroup of phytoestrogens, natural plant substances with structure similar to 17-beta-estradiol and capable of binding to estrogen receptors (ERs). Isoflavones possess higher affinity to ERbeta than to ERalpha and may have a potency to activate both genomic and non-genomic estrogen signaling pathways. In addition, isoflavones interact with the metabolism of steroid hormones. Therefore, the actions of isoflavones are rather complex and may be related to large number of factors, which are not satisfactorily identified yet. Recently, isoflavones have come into focus of interest due to several reports about their positive effect on human health, in particular prevention of hormone-dependent cancers, cardiovascular diseases, osteoporosis, adverse menopausal manifestations and age-related cognitive decline. Isoflavones may bring new insights into the mechanisms of physiological regulations and increase the possibilities of medical interventions.
- MeSH
- fytoestrogeny chemie terapeutické užití MeSH
- isoflavony chemie terapeutické užití MeSH
- kardiovaskulární nemoci farmakoterapie MeSH
- lidé MeSH
- nádory farmakoterapie MeSH
- postmenopauzální osteoporóza farmakoterapie MeSH
- signální transdukce účinky léků MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Názvy látek
- fytoestrogeny MeSH
- isoflavony MeSH
The antidiabetic and antioxidative effect of osajin was monitored under the conditions of alloxan-induced diabetes mellitus in an in vivo experiment. The animals were divided by random selection into 2 groups (n=7). The treated group was administered osajin in peroral doses of 10 mg/kg in Avicel, the placebo diabetic group was given only the solution of Avicel, and the last group was intact. Selected laboratory parameters (glucose, urea, cholesterol, antioxidative enzymes, total antioxidative capacity, malondialdehyde in serum, diuresis, total glucose and protein losses through urine) were determined in all animals. Kidney tissue and pancreas samples were taken for histopathological analysis. The findings included a statistically significant increase (p< or =0.05) in the glutathione peroxidase catalytic activity, total antioxidative capacity (p< or =0.01) and a statistically significant decrease (p< or =0.01) on malondialdehyde level in the treated group compared to the placebo group. A statistically highly significant decrease (p< or =0.01) in diuresis, glucose and protein losses through urine were identified in the treated group compared to the placebo group. The superoxide dismutase catalytic activity, urea and cholesterol levels involved non-significant changes. The results of biochemical examination show a protective antidiabetic and antioxidative effect of osajin. The results of histopathological examination correlate with them only partially.
- MeSH
- antioxidancia terapeutické užití MeSH
- benzopyrany terapeutické užití MeSH
- experimentální diabetes mellitus farmakoterapie metabolismus patologie MeSH
- fytoterapie * MeSH
- hypoglykemika terapeutické užití MeSH
- isoflavony terapeutické užití MeSH
- krysa rodu Rattus MeSH
- potkani Wistar MeSH
- rostlinné extrakty terapeutické užití MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- antioxidancia MeSH
- benzopyrany MeSH
- hypoglykemika MeSH
- isoflavony MeSH
- osajin MeSH Prohlížeč
- rostlinné extrakty MeSH
The present 15 days study was undertaken to evaluate the cardioprotective potential of the prenylated isoflavones osajin and pomiferin isolated from the infructences of Maclura pomifera, Moraceae, against ischemia-reperfusion induced injury in rat hearts as a model of antioxidant-based composite therapy. The study was performed on isolated, modified Langendorff-perfused rat hearts and the ischemia of heart was induced by stopping coronary flow for 30 min followed by 60 min of reperfusion (14 ml min(-1)). The Wistar rats were divided into four groups. The first treatment group received osajin (5 mg/kg/day in 0.5% Avicel); the second treatment group received pomiferin (5 mg/kg/day in 0.5% Avicel); the placebo group received only 0.5 Avicel; the last was an untreated control group. Biochemical indicator of oxidative damage-lipid peroxidation product malondialdehyde, antioxidant enzymes - superoxide dismutase, glutathione peroxidase, total antioxidant activity in serum and myocardium were evaluated. The effect of osajin and pomiferin on cardiac function, left ventricular end-diastolic pressure, left ventricular pressure and peak positive +dP/dt ischemia and reperfusion, also was examined. The results demonstrate that osajin and pomiferin attenuates the myocardial dysfunction provoked by ischemiareperfusion. This was confirmed by an increase in both antioxidant enzyme values and total antioxidant activity. The cardioprotection provided by osajin and pomiferin treatment results from the suppression of oxidative stress and this correlates with improved ventricular function.
- MeSH
- benzopyrany terapeutické užití MeSH
- fytoterapie * MeSH
- isoflavony terapeutické užití MeSH
- krysa rodu Rattus MeSH
- myokard metabolismus MeSH
- potkani Wistar MeSH
- reperfuzní poškození myokardu metabolismus patofyziologie prevence a kontrola MeSH
- rostlinné extrakty terapeutické užití MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- benzopyrany MeSH
- isoflavony MeSH
- osajin MeSH Prohlížeč
- pomiferin MeSH Prohlížeč
- rostlinné extrakty MeSH
The aim of this study was to analyze the antioxidative effect of osajin during prophylactic administration. The pathological model for in vivo experiment was the unilateral ischemia-reperfusion of kidney of the laboratory rat. The animals were randomly divided into five groups. Osajin was administrated orally in doses of 5, 10 and 20 mg/kg once a day to three premedicated groups. Placebo--0.5% solution of Avicel--was given to the fourth group and the fifth group was completely intact. The premedication lasted 15 days and subsequently the ischemia of the left kidney was incited in general anaesthesia for 60 min. The reperfusion lasted 10 min and it was finished by blood collection from the left ventricle and the reperfused kidney was recovered. Selected biochemical markers were assessed in blood: superoxide dismutase, glutathion peroxidase, total antioxidative capacity and malondialdehyde. The kidney tissue samples were used for histopathological examination. Laboratory and histopathological results confirmed supposed effects of osajine. The dependence between the effect and the applied dose of osajin was linear. The best biochemical results were reached after administration of osajin at the dose of 5 mg/kg. The best histopathological results were reached after administration of osajin at the dose of 10 mg/kg.
- MeSH
- antioxidancia terapeutické užití MeSH
- benzopyrany terapeutické užití MeSH
- glutathionperoxidasa metabolismus MeSH
- isoflavony terapeutické užití MeSH
- krysa rodu Rattus MeSH
- ledviny enzymologie patologie MeSH
- malondialdehyd metabolismus MeSH
- nemoci ledvin enzymologie patologie prevence a kontrola MeSH
- potkani Wistar MeSH
- reperfuzní poškození enzymologie patologie prevence a kontrola MeSH
- superoxiddismutasa metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antioxidancia MeSH
- benzopyrany MeSH
- glutathionperoxidasa MeSH
- isoflavony MeSH
- malondialdehyd MeSH
- osajin MeSH Prohlížeč
- superoxiddismutasa MeSH
The study aimed to examine the antioxidizing effect of homoisoflavonoid in prophylactic administration under the conditions of renal ischemia-reperfusion in the laboratory rat. The pathological model for the in vivo experiment was unilateral renal ischemia-reperfusion of the laboratory rat. The animals were randomized into 5 groups. Homoisoflavonoid was administered to treated groups orally in doses of 5, 10 and 20 mg/kg once a day in 0.5% Avicel solution. The placebo group received Avicel only, and the intact group was without medication and intervention. On day 15 of the experiment, renal tissue ischemia/reperfusion (60/10 mins) was induced in the treated and placebo groups. Then the animals were exsanguinated, biochemical parameters in the blood (superoxidismutase, glutathionperoxidase, total antioxidizing capacity and malondialdehyde) were assayed, and renal samples were withdrawn for histopathological examination. A biochemical examination demonstrated a dependence of the effect of homoisoflavonoid on the dose administered. An obvious effect was demonstrated in the values of GSHPx, AOC, and MDA. On the other hand, a negative dependence was found between the dose of administered homoisoflavonoid and SOD and GSHPx values. The results of biochemical examination correlate with the histopathological pictures of the renal tissue and support the assumption about a protective effect of homoisoflavonoid under the conditions of artificially induced pathological state--renal tissue ischemia-reperfusion.
- MeSH
- antioxidancia terapeutické užití MeSH
- isoflavony terapeutické užití MeSH
- krysa rodu Rattus MeSH
- ledviny krevní zásobení patologie MeSH
- potkani Wistar MeSH
- reperfuzní poškození patologie prevence a kontrola MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- antioxidancia MeSH
- isoflavony MeSH
The major constituents of fruits of Maclura pomifera are the prenylated isoflavones, osajin (1) and pomiferin (2). Since significant biological activities of extracts from the wood of M. pomifera were previously reported, the peroxynitrite scavenging activity, inhibition of lipid peroxidation, scavenging of DPPH and EROD activity of these two major substances were studied.
- MeSH
- benzopyrany aplikace a dávkování farmakologie terapeutické užití MeSH
- bifenylové sloučeniny MeSH
- cytochrom P-450 CYP1A1 chemie MeSH
- fytoterapie * MeSH
- isoflavony aplikace a dávkování farmakologie terapeutické užití MeSH
- kyselina peroxydusitá chemie MeSH
- lidé MeSH
- Maclura * MeSH
- ovoce MeSH
- peroxidace lipidů účinky léků MeSH
- pikráty chemie MeSH
- rostlinné extrakty aplikace a dávkování farmakologie terapeutické užití MeSH
- scavengery volných radikálů aplikace a dávkování farmakologie terapeutické užití MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1,1-diphenyl-2-picrylhydrazyl MeSH Prohlížeč
- benzopyrany MeSH
- bifenylové sloučeniny MeSH
- cytochrom P-450 CYP1A1 MeSH
- isoflavony MeSH
- kyselina peroxydusitá MeSH
- osajin MeSH Prohlížeč
- pikráty MeSH
- pomiferin MeSH Prohlížeč
- rostlinné extrakty MeSH
- scavengery volných radikálů MeSH
Evidence has been accumulated that some phytoestrogens act as protective factors against development of cancer and also cardiovascular diseases. These are phytoestrogens of isoflavone and lignane series, found especially in soy. Beneficial effect of these compounds may be explained by a complexity of their actions at various levels: they interact with estrogen receptors, some of them are inhibitors of the key enzymes responsible in the final effect for cell growth and proliferation, and, due to their chemical nature they are scavengers of free radicals. In the presented work the authors, in collaboration with Finnish partners from the University of Helsinki, developed original immunoassays for determination of main phytoestrogens of isoflavone series--daidzein, genistein, formononoetin, biochanin A, their metabolite equal and lignane enterolactone. The methods are sensitive enough for follow-up of actual levels of phytoestrogens in serum. By using these methods, the levels of phytoestrogens in Czech population have been established. The group of patients suffering from osteoporosis has been investigated, too. Significantly lower levels of isoflavonoids in comparison with sex- and age-matched healthy subject have been found in the patients. The methods have also enabled us to follow up the dynamics of these compounds in the organism, as well as to determine their content in food and its sources. The original detection and quantification of four above mentioned isoflavonoids in beer is an example.
- MeSH
- antikarcinogenní látky krev terapeutické užití MeSH
- estrogeny nesteroidní krev terapeutické užití MeSH
- isoflavony krev terapeutické užití MeSH
- lidé MeSH
- lignany krev terapeutické užití MeSH
- nádory prevence a kontrola MeSH
- postmenopauzální osteoporóza krev MeSH
- radioimunoanalýza MeSH
- rostliny * MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- antikarcinogenní látky MeSH
- estrogeny nesteroidní MeSH
- isoflavony MeSH
- lignany MeSH
