Our study concerned the findings that rat and rabbit heart transplants do not survive after six hours. They become dark, hard and fail to contract within 2 min after reperfusion and never regain their function. We tested the supplementation of solutions for heart transplant preservation with tetrahydrobiopterin (H4B) and L-arginine (L-ARG) to maintain the oxidative and reductive domains of the endocardial NO synthase. We decided to study the excised rabbit hearts preserved in Hank's balanced salt solution (HBSS) at 0 degrees C supplemented with different concentrations of H4B (0, 1, 5, 10 or 100 microM). At desired time intervals, successive pieces stored in the above solutions were warmed to rabbit body temperature in 4 ml of HBSS and maximally agonized by direct application of 20 microl of 200 microM bradykinin (or other agonist) onto the exposed endocardium. Nitric oxide bursts were monitored with a porphyrinic NO sensor lying on the exposed endocardium. Our goal was to find the lowest H4B concentration which would maximally agonize NO. and prolong the time of heart preservation to more than 6 hours. Ten microM are a minimum H4B concentration which achieves maximum prolongation of heart preservation time up to 90 hours. This effect was based upon maximal potentiation of NO. release and minimizing of superoxide production.

• MeSH
• časové faktory MeSH
• donory oxidu dusnatého MeSH
• isotonické roztoky MeSH
• králíci MeSH
• krysa rodu Rattus MeSH
• roztoky pro uchovávání orgánů * MeSH
• srdce * MeSH
• transplantace srdce * MeSH
• uchovávání tkání * MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• krysa rodu Rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• donory oxidu dusnatého MeSH
• Hanks Balanced Salt Solution MeSH Prohlížeč
• isotonické roztoky MeSH
• roztoky pro uchovávání orgánů * MeSH

The preservation of donor lungs and the effort to safely extend ischemic time while maintaining function is an important topic that the transplant community has been addressing for a long time. Recent publications, mainly from the Toronto team, have fundamentally influenced the existing standard of optimal preservation conditions, and their results provide a scientific basis for the shift from ice preservation to con-trolled hypothermia. Optimal preservation conditions are a necessary prerequisite for the safe extension of ischemic time. This brings additional potential for the development of the field and the possibility to improve the availability of lung transplantations and their outcomes. This review summarizes the key findings in the area of donor lung preservation from the first experimental attempts conducted 30 years ago to recent studies and discusses the various aspects that the change in preservation standard has influenced or is likely to influence.

• Klíčová slova
• Lung transplantation, donor lung preservation, ischemic time,
• MeSH
• chlazení MeSH
• dárci tkání MeSH
• led MeSH
• lidé MeSH
• transplantace plic * MeSH
• uchovávání orgánů * metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• led MeSH

The function normothermally perfused isolated liver will be determined in our future experiments for the assessment of the quality of previous hypothermic preservation performed by means of various preservation solutions. As the opinions on the optimal storage temperature of organs differ, the aim of the present work was to find a proper storage temperature which would be suitable for our experiments. A comparison of liver preservation using simplified UW solution (UWs) at various temperatures was performed. During the isolation the liver was flushed by 50 ml of UWs through the portal vein and stored for 24 h either at 0 degree or 10 degrees C. The isolated liver was then perfused in a recirculation manner at 37 degrees C. The livers that were stored at 0 degree C showed only mild injury resulting in a slower bile flow rate in comparison with the control group without preservation; the results were only marginally significantly different. The livers that were stored at 10 degrees C showed more pronounced injury (higher portal resistance, higher ALT and AST activity in perfusate, higher water content in livers and slower bile flow) in comparison with other groups. Storage temperature of 0 degree C seems to be more convenient for our future experiments.

• MeSH
• adenosin MeSH
• alopurinol MeSH
• glutathion MeSH
• inzulin MeSH
• játra * metabolismus   patofyziologie   MeSH
• krysa rodu Rattus MeSH
• nízká teplota * MeSH
• potkani Wistar MeSH
• rafinosa MeSH
• roztoky pro uchovávání orgánů * MeSH
• transplantace jater MeSH
• uchovávání orgánů * MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• adenosin MeSH
• alopurinol MeSH
• glutathion MeSH
• inzulin MeSH
• rafinosa MeSH
• roztoky pro uchovávání orgánů * MeSH
• University of Wisconsin-lactobionate solution MeSH Prohlížeč

The objective of the work was to find an optimal preservation medium for short-term preservation of venous grafts which could be subsequently used to line metal stents. The external jugular vein of dogs (n = 15) was removed surgically, divided into portions and immersed into preservation media. For hypothermic preservation (+4 degrees C) solutions of Optisol (Chiron, USA), University of Wisconsin (Baxter, USA), Eurocollins (Fresenius, GFR) and saline (Bieffe Medital, Italy) were used. For normothermic preservation (+37 degrees C) in an atmosphere with 5% CO2 Dulbecc's medium for tissue cultures (Sigma, USA) was used. During hypothermic preservation the specimens were kept for 24 hours, 3 and 7 days, during normothermic preservation in Dulbecc s medium also for 24 hours, 3 and 7 days. The specimens were evaluated by light microscopy and raster electron microscopy. The results revealed that minimal changes on the endothelia of venous grafts occurred during normothermic preservation in Dulbecc's medium where after 7 days the endothelium did not become detached and the vitality of cells did not change. During hypothermic preservation the solution of Wisconsin University proved most suitable. By addition of 5% human albumin to this solution it proved possible moreover to reduce pyknosis of the endothelial cell nuclei. The specimens kept in saline displayed deformities of the nuclei, oedema and loss of endothelia incl. incipient oedema of the cellular wall already after 24 hours of hypothermic preservation. The authors consider the use of this solution unsuitable for preservation.

• MeSH
• cévní endotel ultrastruktura   MeSH
• psi MeSH
• roztoky pro uchovávání orgánů * MeSH
• teplota MeSH
• uchovávání orgánů * MeSH
• venae jugulares cytologie   MeSH
• vény cytologie   transplantace   MeSH
• zvířata MeSH
• Check Tag
• psi MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• roztoky pro uchovávání orgánů * MeSH

Clinical success of small bowel transplantation depends on quality of the preservation small bowel graft which is notoriously sensitive to ischemia. There is still no general agreement as to which segment of the small bowel is preferred (jejunum or ileum) for clinical use. In our study, using a light microscopy and concentrations of tissue serotonin-positive cells, we tried to identify a part of the human intestine, which is more resistant to preservation injury sustained by HTK preservation solution with 1-24 hr of cold ischemia. Statistical analysis of both parameters did not reveal any significant differences between the jejunum and ileum. According to our data, there is no difference between jejunal and ileal grafts in susceptibility to ischemic injury due to cold ischemia within 24 hours when using HTK preservation solution. A significant difference was observed in histological pictures only after 12-hour of cold ischemia in both groups (jejunum and ileum) (Fig. 2, Ref. 11). Full Text (Free, PDF) www.bmj.sk.

• MeSH
• chlorid draselný MeSH
• dospělí MeSH
• glukosa MeSH
• imunohistochemie MeSH
• ischemie patologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mannitol MeSH
• prokain MeSH
• roztoky pro uchovávání orgánů MeSH
• senioři MeSH
• serotonin metabolismus   MeSH
• studená ischemie MeSH
• techniky in vitro MeSH
• tenké střevo metabolismus   patologie   transplantace   MeSH
• uchovávání orgánů * MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• Bretschneider cardioplegic solution MeSH Prohlížeč
• chlorid draselný MeSH
• glukosa MeSH
• mannitol MeSH
• prokain MeSH
• roztoky pro uchovávání orgánů MeSH
• serotonin MeSH

BACKGROUND: Early postoperative complications after small bowel transplantation (SBT) including endotoxemia, bacterial translocations, and stimulation of the recipient's immune response have been attributed to preservation injury. Small intestine is notoriously sensitive to ischemia and there is still no general agreement as to which segment of the small bowel is preferred (jejunum or ileum) for clinical use. AIM OF STUDY: In our study, using light microscopy and concentrations of tissue serotonin-positive cells we sought to identify the part of the human intestine, which is more resistant to preservation injury sustained by HTK preservation solution with 1-24 hr cold ischemia time. RESULTS: Statistical analysis of both parameters did not reveal any significant differences between the jejunum and ileum. CONCLUSIONS: Judging by our data, there is no difference between jejunal and ileal grafts in susceptibility to ischemic injury due to cold ischemia within 24 hours when using HTK preservation solution. Significant difference was observed in histological pictures only after 12-hour of cold ischemia time in both experimental groups (jejunum and ileum).

• MeSH
• dospělí MeSH
• ileum patologie   transplantace   MeSH
• jejunum patologie   transplantace   MeSH
• lidé středního věku MeSH
• lidé MeSH
• reperfuzní poškození komplikace   patologie   MeSH
• senioři MeSH
• střevní sliznice patologie   MeSH
• studená ischemie škodlivé účinky   MeSH
• transplantace orgánů škodlivé účinky   metody   MeSH
• uchovávání orgánů * škodlivé účinky   metody   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH

Corneal stromal lenticule is a part of corneal stroma, which can be created by manual dissection, by femtosecond laser from the donor cornea, but chiefly it is a waste product of a refractive procedure ReLex SMILE (Small Incision Lenticule Extraction). Corneal lenticule has a huge potential in corneal surgery. In recent years, many studies have been published to show the possibility to use this tissue to treat corneal defects, as well as in refractive surgery. Thanks to the quantity of lenticules which arise every day during SMILE operations, this tissue is much more accessible than any other kind of corneal tissue. According to the experience with lenticule implantation in animal models, or even human patients, lenticule implantation is considered safe, reversible method, which is not associated with immune rejection or other severe complications. However, the crucial step before the process of lenticule implantation, is proper preservation of this tissue. Donor corneal tissue containing endothelium is usually preserved in hypothermia and then usable maximally for two weeks. Newer methods such as organ culture storage and use of a sterile cornea prolong the time of usability of the tissue. The possibilities for corneal lenticule storage are theoretically wider thanks to the fact, that we do not need to preserve fragile cellular structures. Besides the storage in hypothermia, other preserving methods such as cryopreservation and storage after decellularization have been tested. This review aimed to examine the current literature that describes possible methods of corneal lenticule preservation. A comprehensive search was created based on articles published in English on PubMed.gov, Cochranelibrary.com and Scopus.com using following keywords: corneal lenticule preservation, corneal lenticule storage, cold storage corneal lenticule, corneal lenticule cryopreservation till 2020.

• Klíčová slova
• Cold storage corneal lenticule, Corneal lenticule cryopreservation, Corneal lenticule implantation, Corneal lenticule preservation, Corneal lenticule storage, Keratophakia,
• MeSH
• hypotermie * MeSH
• kryoprezervace MeSH
• lidé MeSH
• rohovka chirurgie   MeSH
• rohovkový endotel MeSH
• stroma rohovky * chirurgie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

BACKGROUND: The two preservation solutions most commonly used in human transplantation surgery are University of Wisconsin (UW) and Custodiol (histidine-tryptophan-ketoglutarate; HTK). The aim of our study was to compare the protective effect of UW and HTK solutions on preservation-induced injury of jejunal grafts, as evaluated by the histological changes (semiquantitative method) and small bowel mucosal serotonin levels (as a possible new quantitative method). METHODS: Male Wistar rats (n = 50) weighing 316 +/- 52 g were divided into two main groups according to which preservation solution was used, i.e. UW (n = 25) or HTK (n = 25), and each of these groups was divided into five subgroups according to cold ischemic time (0, 1, 6, 9 and 12 h). Jejunal mucosa biopsy specimens were obtained to determine the serotonin concentration in mucosa and for standard light histology. To grade histological changes in mucosa, Park's small bowel injury grading system was used. RESULTS: Histological examination revealed that injury increased with cold ischemic time in the UW as well as in the HTK group, and there were no significant differences in injury between the two groups, except for the 6-hour cold ischemic period (p < 0.05), when HTK-preserved grafts showed a lower degree of injury (0.97 +/- 0.41) compared with UW-preserved grafts (1.25 +/- 0.39). The mucosal serotonin concentration decreased with cold ischemic time in both groups, and there were significant differences (p < 0.05) in concentrations between the groups after 9 and 12 h of cold ischemia. A significantly higher concentration was measured in grafts preserved in UW solution at these time points. CONCLUSION: The concentration of mucosal serotonin in rat small bowel grafts preserved for 9 and 12 h in UW preservation solution was significantly higher than that in HTK solution. These findings indicate a better protective effect of UW solution on small bowel injury after 9 h of cold ischemia.

• MeSH
• adenosin farmakologie   MeSH
• alopurinol farmakologie   MeSH
• chlorid draselný farmakologie   MeSH
• glukosa farmakologie   MeSH
• glutathion farmakologie   MeSH
• inzulin farmakologie   MeSH
• jejunum metabolismus   patologie   transplantace   MeSH
• krysa rodu Rattus MeSH
• mannitol farmakologie   MeSH
• nízká teplota MeSH
• potkani Wistar MeSH
• prokain farmakologie   MeSH
• rafinosa farmakologie   MeSH
• reperfuzní poškození patologie   prevence a kontrola   MeSH
• roztoky pro uchovávání orgánů farmakologie   MeSH
• serotonin metabolismus   MeSH
• střevní sliznice metabolismus   patologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• adenosin MeSH
• alopurinol MeSH
• Bretschneider cardioplegic solution MeSH Prohlížeč
• chlorid draselný MeSH
• glukosa MeSH
• glutathion MeSH
• inzulin MeSH
• mannitol MeSH
• prokain MeSH
• rafinosa MeSH
• roztoky pro uchovávání orgánů MeSH
• serotonin MeSH
• University of Wisconsin-lactobionate solution MeSH Prohlížeč

OBJECTIVE: One of the possible risks of sinonasal malignancy is its possible spread in the orbit. However, there is no clear consensus among the different departments as to whether it is necessary to exenterate the orbit in limited tumorous infiltration of periorbital fat. The purpose of the study was to demonstrate that periorbital infiltration and periorbital fat invasion without involvement of deeper orbital tissues are not the indication of orbital exenteration. MATERIALS AND METHODS: Retrospective analysis was performed over a 17-year period of patients undergoing surgical treatment for sinonasal malignancy with histologically verified periorbital infiltration or deeper invasion into the orbit. A total of 32 patients were included in the study. For each group, the following data were analysed: sex, age, preoperative imaging studies, histological findings, site of origin, stage, surgical reconstruction, oncological treatment, survival, cause of death, number of recurrences in the orbit and functional status of preserved eyes. RESULTS: Based on our criteria for orbital exenteration, orbital preservation was feasible in 18 patients. Orbital exenteration was performed in 14 patients with deeper tumor infiltration. There was a statistically insignificant difference in survival between the two groups. The 5-year overall survival (OS) was 44% for the orbital preservation group (only 2 patients died from local tumor recurrence) and 34% for the orbital exenteration group. The groups did not differ in other observed factors other than the extent of orbital infiltration. In 11 (61.1%) patients, vision was without significant change after radiation therapy. In 2 (11.1%) patients, visual function was impaired due to diplopia. 5 (27.8%) patients had severely impaired vision due to optic nerve atrophy after radiation therapy. CONCLUSIONS: Our results show a relatively high survival rate in the group of patients with orbital preservation with a high chance of vision preservation, which justifies our approach to orbital preservation even in some tumors with periorbital infiltration.

• Klíčová slova
• Orbital exenteration, Orbital preservation, Periorbital infiltration, Sinonasal malignancy, Survival, Vision,
• MeSH
• dospělí MeSH
• eviscerace orbity MeSH
• invazivní růst nádoru * MeSH
• léčba šetřící orgány metody   MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádory orbity * patologie   chirurgie   MeSH
• nádory vedlejších dutin nosních * patologie   chirurgie   MeSH
• orbita patologie   MeSH
• retrospektivní studie MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• staging nádorů MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

The optimal pressure in the renal artery during initial perfusion with Collins' solution by spontaneous pressure in canine kidneys is 40-50 cm H2O and in human kidneys 40-60 cm H2O. An average-sized human kidney can be perfused by a full current which is limited only by the lumen of the infusion set. In smaller than average-sized human kidneys the current must be reduced to diminish the pressure in the renal artery to the optimal value. In prolonged hypothermal perfusion by means of a Gambro apparatus it will be necessary to assess the optimal pressure by direct measurement in the renal artery in intact average-sized human kidneys because the pressure reading on the manometer is not identical with the optimal pressure in the renal artery.

• MeSH
• arteria renalis MeSH
• homologní transplantace MeSH
• krevní tlak MeSH
• ledviny anatomie a histologie   MeSH
• perfuze metody   MeSH
• psi MeSH
• teplota MeSH
• transplantace ledvin * MeSH
• uchovávání orgánů * MeSH
• uchovávání tkání * MeSH
• zvířata MeSH
• Check Tag
• psi MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH