This paper proposes a novel Fuzzy-MPDTC control applied to a fuel cell battery electric vehicle whose traction is ensured using a permanent magnet synchronous motor (PMSM). On the traction side, model predictive direct torque control (MPDTC) is used to control PMSM torque, and guarantee minimum torque and current ripples while ensuring satisfactory speed tracking. On the sources side, an energy management strategy (EMS) based on fuzzy logic is proposed, it aims to distribute power over energy sources rationally and satisfy the load power demand. To assess these techniques, a driving cycle under different operating modes, namely cruising, acceleration, idling and regenerative braking is proposed. Real-time simulation is developed using the RT LAB platform and the obtained results match those obtained in numerical simulation using MATLAB/Simulink. The results show a good performance of the whole system, where the proposed MPDTC minimized the torque and flux ripples with 54.54% and 77%, respectively, compared to the conventional DTC and reduced the THD of the PMSM current with 53.37%. Furthermore, the proposed EMS based on fuzzy logic shows good performance and keeps the battery SOC within safe limits under the proposed speed profile and international NYCC driving cycle. These aforementioned results confirm the robustness and effectiveness of the proposed control techniques.

• Klíčová slova
• battery, electric vehicle, fuel cell, fuzzy logic, model predictive direct torque control, permanent magnet synchronous motor,
• Publikační typ
• časopisecké články MeSH

OBJECTIVE: Officially licensed hybrid closed-loop systems are not currently available worldwide; therefore, open-source systems have become increasingly popular. Our aim was to assess the safety, feasibility, and efficacy of an open-source hybrid closed-loop system (AndroidAPS) versus SmartGuard® technology for day-and-night glucose control in children under extreme sports conditions. RESEARCH DESIGN AND METHODS: Twenty-two children (16 girls, 6-15 years of age, median HbA1c 56 ± 9 mmol/mol) were enrolled in this pivotal winter sports camp study. The participants were divided into two groups using either the AndroidAPS or SmartGuard technology. Physical exertion was represented by all-day alpine skiing. The primary endpoints were mean glucose level, time below the threshold of 3.9 mmol/L, and time within the target range of 3.9 to 10 mmol/L. RESULTS: The children using the AndroidAPS had significantly lower mean glycemia levels (7.2 ± 2.7 vs. 7.7 ± 2.8 mmol/L; 129.6 ± 49 vs. 138.6 ± 50 mg/dL, P < 0.042) than the children using the SmartGuard. The proportion of time below the target (median 5.0% ± 2.5% vs. 3.0% ± 2.3%, P = 0.6) and in the target zone (63% ± 9.5% vs. 63% ± 18%, P = 0.5) did not significantly differ. The AndroidAPS group experienced more frequent malfunctions of the cannula set (median 0.8 ± 0.4 vs. 0.2 ± 0.4, P = 0.02), which could have affected the results. No significant difference was found in the amount of carbohydrates consumed for the prevention and treatment of hypoglycemia [median 40 ± 23 vs. 25 ± 29 g/(patient ·3 days)]. No episodes of severe hypoglycemia or other serious adverse events were noted. CONCLUSIONS: This pilot study showed that the AndroidAPS system was a safe and feasible alternative to the SmartGuard Technology.

• Klíčová slova
• AndroidAPS, Do-it-yourself systems., Open-source hybrid closed loop,
• MeSH
• cvičení fyziologie   MeSH
• diabetes mellitus 1. typu krev   farmakoterapie   patofyziologie   MeSH
• dítě MeSH
• glykovaný hemoglobin metabolismus   MeSH
• hypoglykemie etiologie   prevence a kontrola   MeSH
• hypoglykemika aplikace a dávkování   MeSH
• inzulin aplikace a dávkování   MeSH
• inzulinové infuzní systémy * MeSH
• krevní glukóza metabolismus   MeSH
• lidé MeSH
• lyžování fyziologie   MeSH
• mladiství MeSH
• pilotní projekty MeSH
• selfmonitoring glykemie přístrojové vybavení   metody   MeSH
• studie proveditelnosti MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Názvy látek
• glykovaný hemoglobin MeSH
• hemoglobin A1c protein, human MeSH Prohlížeč
• hypoglykemika MeSH
• inzulin MeSH
• krevní glukóza MeSH

BACKGROUND: In-house built capillary electrophoresis (CE) systems represent a significant share of laboratory instrumentation. In most of these instruments, sample injection is effected manually with low to moderate precision and requires skilled operators. Although few automated samplers have been previously developed, typically only one sample at a time can be injected. If a series of samples is to be analyzed, manual intervention is required. In the present work, we developed and constructed a fully automated, open source, CE autosampler, able to handle up to 14 different samples that can be used as a modular component of any in-house built CE instrument. RESULTS: An inexpensive, 3D printed, open source, autosampler for CE was developed. The autosampler consists of two parts: an injection unit with carousel containing sample and electrolyte vials and a flushing unit, containing a miniature pressure/vacuum pump. The autosampler is operated by an Arduino Mega microcontroller and an Arduino code written in the laboratory. The injection sequence is entered through a keypad and LCD display by the user. The instrument can operate autonomously for extended periods of time. It was used for fully automated analysis and/or calibration of up to 14 samples with excellent injection repeatability reaching less than 2.7% RSD for peak areas. The sampler performance was tested with two independently built CE instruments, a CE system with contactless conductivity detection (C4D) and a CE system with laser induced fluorescence (LIF) detector. SIGNIFICANCE AND NOVELTY: A novel, 3D printed, Arduino-based autosampler for CE was developed. The autosampler allows autonomous hydrodynamic injection of up to 14 different samples with fully programmable injection sequence, including capillary flushing and high voltage and data acquisition control. It provides the missing instrumental sampling setup for laboratory made CE instruments. It can be simply constructed based on the open-source blueprints in any laboratory and be a useful and time-saving add-on to any modular CE instrument.

• Klíčová slova
• Arduino, Autosampler, Capillary electrophoresis, Open source hardware and software,
• Publikační typ
• časopisecké články MeSH

AIM: To compare open-source AndroidAPS (AAPS) and commercially available Control-IQ (CIQ) automated insulin delivery (AID) systems in a prospective, open-label, single-arm clinical trial. METHODS: Adults with type 1 diabetes who had been using AAPS by their own decision entered the first 3-month AAPS phase then were switched to CIQ for 3 months. The results of this treatment were compared with those after the 3-month AAPS phase. The primary endpoint was the change in time in range (% TIR; 70-80 mg/dL). RESULTS: Twenty-five people with diabetes (mean age 34.32 ± 11.07 years; HbA1c 6.4% ± 3%) participated in this study. CIQ was comparable with AAPS in achieving TIR (85.72% ± 7.64% vs. 84.24% ± 8.46%; P = .12). Similarly, there were no differences in percentage time above range (> 180 and > 250 mg/dL), mean sensor glucose (130.3 ± 13.9 vs. 128.3 ± 16.9 mg/dL; P = .21) or HbA1c (6.3% ± 2.1% vs. 6.4% ± 3.1%; P = .59). Percentage time below range (< 70 and < 54 mg/dL) was significantly lower using CIQ than AAPS. Even although participants were mostly satisfied with CIQ (63.6% mostly agreed, 9.1% strongly agreed), they did not plan to switch to CIQ. CONCLUSIONS: The CODIAC study is the first prospective study investigating the switch between open-source and commercially available AID systems. CIQ and AAPS were comparable in achieving TIR. However, hypoglycaemia was significantly lower with CIQ.

• Klíčová slova
• continuous glucose monitoring, glycaemic control, hypoglycaemia, insulin pump therapy, type 1 diabetes,
• MeSH
• diabetes mellitus 1. typu * farmakoterapie   MeSH
• dospělí MeSH
• glykovaný hemoglobin MeSH
• hypoglykemika terapeutické užití   MeSH
• inzulin terapeutické užití   MeSH
• inzulinové infuzní systémy MeSH
• inzuliny * MeSH
• krevní glukóza MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• prospektivní studie MeSH
• selfmonitoring glykemie metody   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky MeSH
• Názvy látek
• glykovaný hemoglobin MeSH
• hypoglykemika MeSH
• inzulin MeSH
• inzuliny * MeSH
• krevní glukóza MeSH

The circadian variation of sensory and motor symptoms with increasing severity in the evening and at night is a key diagnostic feature/symptom of the restless legs syndrome (RLS). Even though many neurological diseases have shown a strong nexus between motor and cognitive symptoms, it has remained unclear whether cognitive performance of RLS patients declines in the evening and which neurophysiological mechanisms are affected by the circadian variation. In the current study, we examined daytime effects (morning vs. evening) on cognitive performance in RLS patients (n = 33) compared to healthy controls (n = 29) by analyzing flanker interference effects in combination with EEG and source localization techniques. RLS patients showed larger flanker interference effects in the evening than in the morning (p = .023), while healthy controls did not display a comparable circadian variation. In line with this, the neurophysiological data showed smaller N1 amplitudes in RLS patients compared to controls in the interfering task condition in the evening (p = .042), but not in the morning. The results demonstrate diurnal cognitive changes in RLS patients with intensified impairments in the evening. It seems that not all dopamine-regulated cognitive processes are altered in RLS and thus show daytime-dependent impairments. Instead, the daytime-related cognitive impairment emerges from attentional selection processes within the extra-striate visual cortex, but not from later cognitive processes such as conflict monitoring and response selection.

• Klíčová slova
• Attentional selection, Circadian variation, Cognition, EEG, Flanker interference effect, Restless legs syndrome (RLS),
• MeSH
• cirkadiánní rytmus fyziologie   MeSH
• dospělí MeSH
• elektroencefalografie MeSH
• kognice fyziologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• pozornost fyziologie   MeSH
• senioři MeSH
• syndrom neklidných nohou patofyziologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

To characterize the source contributions of chemicals of emerging concern (CECs) from different aquatic environments of Taiwan, we collected water samples from different aquatic systems, which were screened for 30 pharmaceuticals and illicit drugs. The total estimated mass loadings of CECs were 23.1 g/d in southern aquatic systems and 133 g/d in central aquatic systems. We developed an analytical framework combining pollutant fingerprinting, hierarchical cluster analysis (HCA), and principal component analysis with multiple linear regression (PCA-MLR) to infer the pharmaco-signature and source contributions of CECs. Based on this approach, we estimate source contributions of 62.2% for domestic inputs, 16.9% for antibiotics application, and 20.9% for drug abuse/medication in southern aquatic system, compared with 47.3% domestic, 35.1% antibiotic, and 17.6% drug abuse/medication inputs to central aquatic systems. The proposed pharmaco-signature method provides initial insights into the profile and source apportionment of CECs in complex aquatic systems, which are of importance for environmental management.

• Klíčová slova
• Emerging contaminants, Fingerprinting, Mass loading, Risk quotient, Source apportionment,
• MeSH
• analýza hlavních komponent MeSH
• chemické látky znečišťující vodu analýza   chemie   MeSH
• lineární modely MeSH
• monitorování životního prostředí metody   MeSH
• multivariační analýza MeSH
• odpadní voda chemie   MeSH
• řeky chemie   MeSH
• řízení kvality MeSH
• shluková analýza MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Taiwan MeSH
• Názvy látek
• chemické látky znečišťující vodu MeSH
• odpadní voda MeSH

AIM: The aim of this retrospective study is to compare two methods of optic disc drusen imaging in pediatric patients - new swept source OCT technology with the B-scan ultrasonography, which has been assumed to be a gold standard in diagnosing optic disc drusen, and to compare pRNFL thickness in children with optic disc drusen and control group. METHODS: 14 eyes of 8 pediatric patients in whom optic disc drusen were confirmed by either B-scan ultrasonography, or swept-source OCT, were included in the study. We compared the sensitivity of these two imaging methods. Then we compared an average pRNFL thickness and pRNFL thickness in all four quadrants of our study group with the control group. Two statistical methods were used in data analysis - Mann-Whitney a Kruskal-Wallis test. RESULTS: The difference between SS-OCT and B-scan ultrasonography was not statistically significant in diagnosing optic disc drusen. Average pRNFL thickness was 135.29 ± SD 31.2 μm in eyes with optic disc drusen, which is 24.15 % higher than in control group (p = 0.00214; p = 0.00207). pRNFL thickness of temporal (p = 0.0001; p = 0.0001), superior (p = 0.03486; p = 0.03361) and inferior (p = 0.00652; p = 0.00627) quadrant was statistically significantly higher in comparison with healthy controls, whereas the difference of pRNFL thickness in nasal quadrant was not statistically significant between the study and control group (p = 0.09692; p = 0.0947). CONCLUSION: Swept source OCT is a promising new "gold standard" in optic disc drusen diagnostics in pediatric patients. An increase in pRNFL thickness values does not always confirm papilloedema as optic disc drusen may be the cause. Direct visualisation of optic disc drusen by swept source OCT can aid in differentiation from true papilloedema.

• Klíčová slova
• Optic disc drusen, RNFL, swept source OCT, ultrasonography, ultrasound,
• MeSH
• dítě MeSH
• drúzy zrakového nervu * diagnóza   diagnostické zobrazování   MeSH
• lidé MeSH
• městnavá papila * MeSH
• nervová vlákna MeSH
• optická koherentní tomografie MeSH
• retrospektivní studie MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

The carbon source is essential as an electron donor in the heterotrophic denitrification process. When there is a lack of organic carbon sources in the system, an external carbon source is needed to improve denitrification efficiency. This review compiles the effects of liquid, solid and gaseous carbon sources on denitrification. Sodium acetate has better denitrification efficiency and is usually the first choice for external carbon sources. Fermentation by-products have been demonstrated to have the same denitrification efficiency as sodium acetate. Compared with cellulose-rich materials, biodegradable polymers have better and more stable denitrification performance in solid-phase nitrification, but their price is higher than the former. Methane as a gaseous carbon source is studied mainly by aerobic methane oxidation coupled with denitrification, which is feasible using methane as a carbon source. Liquid carbon sources are better controlled and utilized than solid carbon sources and gaseous carbon sources. In addition, high carbon to nitrogen ratio and hydraulic retention time can promote denitrification, while high dissolved oxygen (DO>2.0 mg L-1) will inhibit the denitrification process. At the same time, high temperature is conducive to the decomposition of carbon sources by microorganisms. This review also considers the advantages and disadvantages of different carbon sources and cost analysis to provide a reference for looking for more economical and effective external carbon sources in the future.

• Klíčová slova
• External carbon source, Heterotrophic denitrification, Nitrogen removal, Sewage treatment,
• MeSH
• bioreaktory MeSH
• denitrifikace MeSH
• dusík MeSH
• heterotrofní procesy MeSH
• nitrifikace MeSH
• odpadní voda MeSH
• odpadní vody * MeSH
• uhlík * MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• dusík MeSH
• odpadní voda MeSH
• odpadní vody * MeSH
• uhlík * MeSH

Dealing with the islanded operation of a microgrid (MG), the micro sources must cooperate autonomously to regulate the voltage and frequency of the local power grid. Droop controller-based primary control is a method typically used to self-regulate voltage and frequency. The first problem of the droop method is that in a steady state, the microgrid's frequency and voltage deviate from their nominal values. The second concerns the power-sharing issue related to mismatched power line impedances between Distribution Generators (DGs) and MGs. A Secondary Control Unit (SCU) must be used as a high-level controller for droop-based primary control to address the first problem. This paper proposed a decentralized SCU scheme to deal with this issue using optimized PI controllers based on a Genetic Algorithm (GA) and Artificial Neural Networks (ANNs). The GA provides the appropriate adjustment parameters for all adopted PI controllers in the primary control-based voltage and current control loops and SCU-based voltage and frequency loops. ANNs are additionally activated in SCUs to provide precise online control parameter modification. In the proposed control structure, a virtual impedance method is adopted in the primary control scheme to address the power-sharing problem of parallel DGs. Further, in this paper, one of the main objectives includes electricity transmission over long distances using Low-Voltage DC Transmission (LVDCT) systems to reduce power losses and eradicate reactive power problems. Voltage Source Inverters (VSIs) are adopted to convert the DC electrical energy into AC near the consumer loads. The simulation results illustrated the feasibility of the proposed solutions in restoring voltage and frequency deviations, reducing line losses, as well as achieving active and reactive power sharing among the DGs connected to the MG.

• Klíčová slova
• artificial neural network, distribution generators, genetic algorithm, microgrid, power sharing, secondary control, virtual impedance,
• MeSH
• elektrická impedance MeSH
• elektřina * MeSH
• neuronové sítě (počítačové) MeSH
• počítačová simulace MeSH
• zdroje elektrické energie * MeSH
• Publikační typ
• časopisecké články MeSH

PURPOSE: Neuroimaging pipelines have long been known to generate mildly differing results depending on various factors, including software version. While considered generally acceptable and within the margin of reasonable error, little is known about their effect in common research scenarios such as inter-group comparisons between healthy controls and various pathological conditions. The aim of the presented study was to explore the differences in the inferences and statistical significances in a model situation comparing volumetric parameters between healthy controls and type 1 diabetes patients using various FreeSurfer versions. METHODS: T1- and T2-weighted structural scans of healthy controls and type 1 diabetes patients were processed with FreeSurfer 5.3, FreeSurfer 5.3 HCP, FreeSurfer 6.0 and FreeSurfer 7.1, followed by inter-group statistical comparison using outputs of individual FreeSurfer versions. RESULTS: Worryingly, FreeSurfer 5.3 detected both cortical and subcortical volume differences out of the preselected regions of interest, but newer versions such as FreeSurfer 5.3 HCP and FreeSurfer 6.0 reported only subcortical differences of lower magnitude and FreeSurfer 7.1 failed to find any statistically significant inter-group differences. CONCLUSION: Since group averages of individual FreeSurfer versions closely matched, in keeping with previous literature, the main origin of this disparity seemed to lie in substantially higher within-group variability in the model pathological condition. Ergo, until validation in common research scenarios as case-control comparison studies is included into the development process of new software suites, confirmatory analyses utilising a similar software based on analogous, but not fully equivalent principles, might be considered as supplement to careful quality control.

• Klíčová slova
• FreeSurfer, Magnetic resonance imaging, Repeatability, Volumetry,
• MeSH
• lidé MeSH
• magnetická rezonanční tomografie * metody   MeSH
• mozek diagnostické zobrazování   patologie   MeSH
• neurozobrazování * metody   MeSH
• počítačové zpracování obrazu MeSH
• software MeSH
• studie případů a kontrol MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH