240 serum samples originating from various AIDS risk groups were tested for HIV-antibodies comparing an immunofluorescence test with the Western Blot and ELISA methods. In 95% of cases the results of the Western Blot and the immunofluorescence test were in accordance. In 12 samples the immunofluorescence test yielded positive results whereas there was no reaction in the Western Blot. Four of the sera were confirmed by the RIPA and yielded results which were in conformity with the immunofluorescence findings. A noteworthy case is a patient under observation since 1975 who had repeatedly shown negative Western Blot results but positive immunofluorescence responses and who had developed already marked clinical manifestations of AIDS. These findings indicate that the immunofluorescence test, employed as a confirmation method, is comparable to the Western Blot in its specificity (95.6%) but superior as far as sensitivity is concerned. Simplicity of performance and duration of test are the same for the immunofluorescence test and ELISA.

• MeSH
• AIDS diagnóza   MeSH
• dospělí MeSH
• ELISA MeSH
• fluorescenční protilátková technika MeSH
• HIV séropozitivita diagnóza   MeSH
• imunosorpční techniky MeSH
• lidé MeSH
• protilátky virové analýza   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• srovnávací studie MeSH
• Názvy látek
• protilátky virové MeSH

In none of the examined children, regardless of whether they manifested negative (17 children) or positive (25 children) seroconversion after vaccination with live measles vaccine, interferon activity could be demonstrated in the serum (starting with 1:4 dilution) withdrawn at the time of the vaccine administration.

• MeSH
• dítě MeSH
• interferony krev   MeSH
• lidé MeSH
• protilátky virové analýza   MeSH
• spalničková vakcína aplikace a dávkování   MeSH
• tvorba protilátek MeSH
• virus spalniček imunologie   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• interferony MeSH
• protilátky virové MeSH
• spalničková vakcína MeSH

In 37 patients with chronic hepatitis B in the stage of active viral replication the authors tried to assess the time of seroconversion of HBeAg to anti-HBe and HBsAg to anti-HBs (21 men, 13 women and 3 boys, mean age of adults 44 years). During the 8-years investigation period seroconversion of HBeAg occurred in 19 of 37 patients (51.3%) which is an annual rate of seroconversion 6.4%. HBeAg disappeared from serum after a mean period of 82 months (range 29-228 months). After seroconversion of HBeAg the ALT and AST activity declined significantly. Acute exacerbation of hepatitis before seroconversion of HBeAg was recorded in 13 of 37 patients (35%). The mean ALT activity at the peak of exacerbation was 7.93 mu kat/l, the average duration of exacerbation was 9.8 months. Seroconversion of HBsAg occurred in 7 of 37 patients (19%). The mean annual rate of seroconversion of HBsAg was 2.4% after an average period of 98 months. During the investigation period 7 patients died (19%), four of them had a constantly positive HBeAg. The authors conclude that very low annual average seroconversion of HBeAg (6.4%) and HBsAg (2.4%) in patients with chronic hepatitis B is probably caused by previous glucocorticoid treatment.

• MeSH
• chronická nemoc MeSH
• dítě MeSH
• dospělí MeSH
• hepatitida B - antigeny e imunologie   MeSH
• hepatitida B - antigeny povrchové imunologie   MeSH
• hepatitida B - protilátky analýza   MeSH
• hepatitida B imunologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• prospektivní studie MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• hepatitida B - antigeny e MeSH
• hepatitida B - antigeny povrchové MeSH
• hepatitida B - protilátky MeSH

OBJECTIVES: To determine the incidence and laboratory characteristics of primary Toxoplasma gondii infection in HIV-infected individuals. METHODS: This retrospective study was conducted between 1988 and 2012 on a cohort of 1130 HIV-infected patients at the AIDS Center Prague. Toxoplasma serology, standard laboratory parameters, and health status were evaluated at 3-6-month intervals for all patients. RESULTS: The total person-time of follow-up of patients at risk of Toxoplasma seroconversion was 3046.3 years; there were 14 primary T. gondii infections, yielding an incidence rate of 0.0046 (95% confidence interval 0.0027-0.0078). Most of the subjects were clinically asymptomatic, but in one case seroconversion was accompanied by transient cervical lymphadenopathy. The CD4+ T-lymphocyte count geometric mean increased from 418 (95% confidence interval 303-579) cells/μl before seroconversion to 501 (95% confidence interval 363-691) cells/μl after seroconversion (p = 0.004), while other parameters (CD8+ T-lymphocytes, natural killer cells, viral load, beta2-microglobulin, total immunoglobulins) remained unchanged. As compared to the control group, patients with primary toxoplasmosis had higher initial levels of total immunoglobulins IgA and IgG and a tendency to higher CD8+ T lymphocyte counts. CONCLUSIONS: Neither the incidence nor the course of the primary Toxoplasma infection was influenced by the immune status of the patients. Immune parameters of patients with primary Toxoplasma infection did not differ from those of the controls.

• Klíčová slova
• HIV, Incidence, Primary infection, Seroconversion, Toxoplasma gondii,
• MeSH
• dospělí MeSH
• HIV protilátky krev   MeSH
• HIV séropozitivita epidemiologie   imunologie   MeSH
• incidence MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• oportunní infekce doprovázející AIDS epidemiologie   imunologie   MeSH
• počet CD4 lymfocytů MeSH
• retrospektivní studie MeSH
• toxoplazmóza epidemiologie   imunologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• HIV protilátky MeSH

Seroconversion rates of hepatitis B vaccination with increasing antigen doses, in 77 haemodialysis (HD) patients were studied in randomly divided two groups. The first group received the standard 20 microg recombinant HBsAg (rHBsAg) dose, and the second group 40 microg at the 0, 1st and 2nd months of HD. At the end of the study, there were no statistically significant differences between the two groups in the response rates of protective antibody, but the response was always higher in the second group. On the other hand, strong positive antibody response (> or = 100 mlU/ml) according to dates of each dose were as 7.4%, 13.1%, 28.9% and 42.2% in the first group and 50.0%, 50.0%, 60.0% and 52.9% in the second group respectively, and there were statistically significant differences between the two groups in the first three months. We concluded that vaccination with higher antigen doses resulted in a stronger antibody response in the early period in our HD patients. No HBV infection was observed in these two groups in the study period.

• MeSH
• dialýza ledvin * MeSH
• ELISA MeSH
• hepatitida B - antigeny povrchové aplikace a dávkování   imunologie   MeSH
• hepatitida B - protilátky biosyntéza   MeSH
• imunologická odpověď na dávku MeSH
• lidé středního věku MeSH
• lidé MeSH
• prospektivní studie MeSH
• rekombinantní proteiny aplikace a dávkování   imunologie   MeSH
• vakcína proti hepatitidě B aplikace a dávkování   imunologie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky MeSH
• randomizované kontrolované studie MeSH
• Geografické názvy
• Turecko MeSH
• Názvy látek
• hepatitida B - antigeny povrchové MeSH
• hepatitida B - protilátky MeSH
• rekombinantní proteiny MeSH
• vakcína proti hepatitidě B MeSH

Although severe acute respiratory syndrome coronavirus 2 messenger ribonucleic acid (SARS-CoV-2 mRNA) vaccines are effective in kidney transplant recipients (KTRs), their immune response to vaccination is blunted by immunosuppression. Other tools enhancing vaccination response are therefore needed. Interestingly, aligning vaccine administration with circadian rhythms (chronovaccination) has been shown to boost immune response. However, its applicability in KTRs, whose circadian rhythms are likely disrupted by immunosuppressants, remains unclear. To assess the impact of vaccination timing on seroconversion in the KTRs population, we analyzed data from 553 virus-naïve KTRs who received 2 doses of messenger ribonucleic acid (mRNA) vaccine. Bayesian logistic regression was employed, adjusting for previously identified predictors of seroconversion, including allograft function, maintenance immunosuppressants, or time since transplantation. SARS-CoV-2 immunoglobulin G (IgG) levels were measured with a median of 47 days after the second dose. The results did not reveal a reliable effect of timing of the first dose but did indicate that earlier timing for the second dose brings a notable benefit-every 1-hour delay in the application was associated with a 16% reduction in the odds of seroconversion (OR 0.84, 95% CI 0.71, 0.998). Similar results were obtained from quantile regression modeling IgG levels. In conclusion, morning vaccination is emerging as a promising and easily implementable strategy to enhance vaccine response in KTRs.

• Klíčová slova
• Bayesian modeling, SARS-CoV-2 mRNA vaccines, chronovaccination, circadian rhythms, immunosuppression, kidney transplantation, seroconversion, vaccination response, vaccination timing,
• Publikační typ
• časopisecké články MeSH

An evaluation of the relationship between predictors and immune response was conducted using data obtained from a clinical trial in 200 Czech healthy adults aged 24-65 years receiving a booster dose of a monovalent tetanus vaccine in 2017. The response was determined from ELISA antibody concentrations of paired sera obtained before and 4 weeks after the immunisation. While all subjects with initial antibody levels 2.2 IU/ml. The immune response was not affected by sex, age, tetanus vaccine type, concomitant medication, related adverse events or post-vaccination period since there were no significant differences in geometric mean concentrations or seroconversion rates. The seroconversion rate of 56% in smokers was significantly lower than that of 73% achieved in non-smokers. Although the seroconversion rates did not differ between individuals with normal or higher body weight, the adjusted odds ratio (1.3; 95% Cl 1.08-1.60) revealed a positive correlation between seroconversion rate and body mass index (BMI). Although the vaccine-induced response was influenced by pre-vaccination antibody levels, smoking or BMI, the booster immunisation against tetanus produced a sufficient response regardless the predictors.

• Klíčová slova
• BMI, pre-vaccination levels, seroconversion rate, smoking, tetanus vaccination,
• MeSH
• dospělí MeSH
• ELISA MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• protilátky bakteriální krev   MeSH
• sekundární imunizace metody   MeSH
• senioři MeSH
• tetanový toxoid aplikace a dávkování   imunologie   MeSH
• tetanus prevence a kontrola   MeSH
• tvorba protilátek * MeSH
• zdraví dobrovolníci pro lékařské studie MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• protilátky bakteriální MeSH
• tetanový toxoid MeSH

INTRODUCTION: The increase in measles cases in early 2019 led to the implementation of several preventive measures focused mainly on health care providers. The study aimed to evaluate the seroprevalence of measles antibodies among employees of a large hospital and, a year apart, the rate of seroconversion in a pilot sample of the revaccinated subjects. METHODS: In 3027 employees of the University Hospital Olomouc, specific immunoglobulin G levels were tested on a voluntary basis. Those with insufficient levels were offered a booster dose. About approximately one year after the booster dose, the same test was performed in a sample of 52 employees. RESULTS: Of the tested subjects with a mean age of 41.8 ± 9.2 years, 54.0% were seropositive. A higher proportion of seropositivity as well as higher absolute values of antibody titers were noted in those born before routine vaccination was introduced in 1969. A total of 80.9% of the seronegative subjects opted for a booster dose. Seroconversion occurred in 73.2% of retested subjects. The relative increase of post-booster antibody titers was moderately correlated with age (r = 0.47, p < 0.05). CONCLUSION: The proportion of seronegative employees of a large hospital reached 46.0%, being higher in younger individuals. Seroconversion occurred in 73.2% of booster dose recipients included in a pilot sample for reanalysis. A statistically significant correlation was noted between the relative increase of antibody titers and age.

• Klíčová slova
• booster dose, measles, seroconversion, seroprevalence,
• MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• nemocnice univerzitní MeSH
• pilotní projekty MeSH
• protilátky virové * MeSH
• séroepidemiologické studie MeSH
• spalničky * epidemiologie   prevence a kontrola   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• protilátky virové * MeSH

22 of 182 sera samples from Nicaragua, 6 of 111 samples from Columbia, and 25 of 388 samples from El Salvador were positive in the ELISA test. 37 of the 53 positive sera samples were positive again in a further ELISA test, and 1 of them was positive in the IFT and Western Blot test.

• MeSH
• ELISA MeSH
• HIV séropozitivita epidemiologie   MeSH
• imunosorpční techniky MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Kolumbie MeSH
• Nikaragua MeSH
• Salvador MeSH

The authors present the trends of some characteristics recorded in a group of subjects examined for HIV antibodies in the AIDS centre of the District Hygiene Station in Karviná in 1993 to 2000. These characteristics reflect the development of activities of the centre. During the period a total of 3733 HIV tests were made, incl. three positive ones. In two instances men with homosexual relations in the case-history were involved, in one case a male heterosexual. The declining trend of the ratio of anonymous examinations is apparent from 58.8% in 1993 to 7.7% in 2000. Parenteral drug users, prostitutes (in particular women) and homosexuals account on a long-term basis for ca 30% of all examinations. Recently this ratio is even higher. Repeated examinations (check-ups) of the same client account for 16.1% of all implemented HIV tests. At least once 384 parenteral drug users were examined, in prostitutes similarly as in men with homosexual relationships the number of subjects examined at least once is ca 200. Repeated tests were made in ca one quarter of all parenteral drug users, ca one third of the prostitutes and ca one fifth of homosexuals. In none of the repeated tests seroconversion to HIV positivity was recorded. To achieve close contact and monitoring of the HIV statute of groups with a risk behaviour despite their existence at the brink of law and despite their natural tendency to hide is real. Without collaborating organizations, without gaining their confidence and in particular without the confidence of the clients this would not be possible.

• MeSH
• dítě MeSH
• dospělí MeSH
• HIV séropozitivita epidemiologie   MeSH
• lidé MeSH
• mladiství MeSH
• rizikové faktory MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH