Vypracovala sa jednoduchá, citlivá a reprodukovateľná metóda dôkazu extracelulárnej β-galaktozidázy. Kalusovou kultúrou, resp. koreňom klíčnych rastlín sekrétovaná β-galaktozidáza hydrolyzuje substrát (6-bróm-2-naftyl-β-D-galaktopyranozid) na β-D-galaktózu a 6-bróm-2-naftol, ktorý po simultánnej azokopulácii s hexazotovaným p-rozanilínom, resp. bázickým fuksínom vytvára červenohnedú vo vode takmer nerozpustnú zlúčeninu (dÍEizóniovú soI). Zafarbenie pod kalusovou kultúrou a okolo nej, ako aj koreňa iďíčnych rastlín je prejavom činnosti extracelulárnej β-galaktozidázy Študovanými objektmi. Intenzita zafarbenia je mierou ich enzýmovej aktivity. Intracelulárne lokalizovaný enzým má majoritné, kým extracelulárna β-galaktozidáza minoritné zastúpenie

A simple, sensitive and reproducible method of detection of extracellular β-galactosidase was worked out. β-Galactosidase secreted by the callus culture, or the roots of sprouting plants, hydrolyzes the substrate (6-bromo-2-naphthyl β-D-galactopyranoside) to produce β-D-galactose and 6-bromo-2-naphthol, which after simultaneous azocoupling with hexazotized p-rosaniline, or basic fuchsine, produce a reddish brown compound, nearly insoluble in water (a diazonium salt). The colouring under the callus culture and around it, as well as the colouring of the roots of the sprouting plants, is a manifestation of the activity of extracellular β-galactosidase by the objects under study. The intensity of the colouring is a measure of their enzymatic activity. The share of intracellularly localized enzyme represents the majority and the share of extracellular β-galactosidase the minority.

Záhrada liečivých rastlín Farmaceutickej fakulty Univ Kom Bratislava

Presentation of beta-galactosidase secretion by plant organs

Prezentácia sekrécie beta-galaktozidázy rastlinnými bunkami = Presentation of beta-galactosidase secretion by plant organs /

Presentation of beta-galactosidase secretion by plant organs /

Lit: 26

Souhrn: eng