PURPOSE: To evaluate the hypothesis that detection of neuroblastoma mRNAs by reverse transcriptase quantitative polymerase chain reaction (RTqPCR) in peripheral blood (PB) and bone marrow aspirates (BM) from children with stage 4 neuroblastoma are clinically useful biomarkers of risk. METHODS: RTqPCR for paired-like homeobox 2b (PHOX2B), tyrosine hydroxylase (TH), and doublecortin (DCX) mRNA in PB and BM of children enrolled onto the High-Risk Neuroblastoma Trial-1 of the European Society of Pediatric Oncology Neuroblastoma Group (HR-NBL1/SIOPEN) was performed at diagnosis and after induction therapy. RESULTS: High levels of TH, PHOX2B, or DCX mRNA in PB or BM at diagnosis strongly predicted for worse event-free survival (EFS) and overall survival (OS) in a cohort of 290 children. After induction therapy, high levels of these mRNAs predicted worse EFS and OS in BM but not in PB. Combinations of mRNAs in BM did not add to the predictive power of any single mRNA. However, in the original (n = 182) and validation (n = 137) PB cohorts, high TH (log10TH > 0.8) or high PHOX2B (log10PHOX2B > 0.28) identify 19% of children as ultrahigh risk, with 5-year EFS and OS rates of 0%; OS rate was 25% (95% CI, 16% to 36%) and EFS rate was 38% (95% CI, 28% to 49%) in the remaining children. The magnitude of reduction in mRNA level between diagnosis and postinduction therapy in BM or PB was not of additional predictive value. CONCLUSION: High levels of TH and PHOX2B mRNA in PB at diagnosis objectively identify children with ultrahigh-risk disease who may benefit from novel treatment approaches. The level of TH, PHOX2B, and DCX mRNA in BM and/or PB at diagnosis might contribute to an algorithm to improve stratification of children for treatment.

Ales Vicha Charles University and University Hospital Motol Prague Czech Republic

Andrei Tchirkov Centre Hospitalier Universitaire Clermont Ferrand and Clermont Université Université d'Auvergne Clermont Ferrand

Andrew D Pearson Institute of Cancer Research Royal Marsden National Health Service Foundation Trust Sutton United Kingdom

Dominique Valteau Couanet Institut Gustave Roussy Villejuif France

Katrien Swerts and Genevieve Laureys University Hospital Ghent Ghent Belgium

Maria 5 Corrias and Sandro Dallorso Gaslini Institute Genoa

Penelope Brock Great Ormond Street Hospital London

Roberto Luksch Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Istituto Nazionale dei Tumori Milano Italy

Ruth Ladenstein Children's Cancer Research Institute St Anna Children's Hospital Vienna Austria

Vassilios Papadakis Agia Sofia Children's Hospital Athens Greece

Virginie F Viprey and Susan A Burchill Leeds Institute of Cancer and Pathology

Walter M Gregory Clinical Trials Research Unit University of Leeds Leeds

Citace poskytuje Crossref.org