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Biological characterization of a novel hybrid copolymer carrier system based on glycogen
M. Jirátová, A. Pospíšilová, M. Rabyk, M. Pařízek, J. Kovář, A. Gálisová, M. Hrubý, D. Jirák,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NV15-25781A
MZ0
CEP - Centrální evidence projektů
- MeSH
- buňky Hep G2 MeSH
- endocytóza MeSH
- fluorescein-5-isothiokyanát aplikace a dávkování farmakokinetika MeSH
- fluorescenční barviva aplikace a dávkování farmakokinetika MeSH
- glykogen aplikace a dávkování farmakokinetika MeSH
- heterocyklické sloučeniny aplikace a dávkování farmakokinetika MeSH
- lidé MeSH
- myši inbrední C57BL MeSH
- nosiče léků aplikace a dávkování farmakokinetika MeSH
- organokovové sloučeniny aplikace a dávkování farmakokinetika MeSH
- polyaminy aplikace a dávkování farmakokinetika MeSH
- tkáňová distribuce MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The effective drug delivery systems for cancer treatment are currently on high demand. In this paper, biological behavior of the novel hybrid copolymers based on polysaccharide glycogen were characterized. The copolymers were modified by fluorescent dyes for flow cytometry, confocal microscopy, and in vivo fluorescence imaging. Moreover, the effect of oxazoline grafts on degradation rate was examined. Intracellular localization, cytotoxicity, and internalization route of the modified copolymers were examined on HepG2 cell line. Biodistribution of copolymers was addressed by in vivo fluorescence imaging in C57BL/6 mice. Our results indicate biocompatibility, biodegradability, and non-toxicity of the glycogen-based hybrid copolymers. Copolymers were endocyted into the cytoplasm, most probably via caveolae-mediated endocytosis. Higher content of oxazoline in polymers slowed down cellular uptake. No strong colocalization of the glycogen-based probe with lysosomes was observed; thus, it seems that the modified externally administered glycogen is degraded in the same way as an endogenous glycogen. In vivo experiment showed relatively fast biodistribution and biodegradation. In conclusion, this novel nanoprobe offers unique chemical and biological attributes for its use as a novel drug delivery system that might serve as an efficient carrier for cancer therapeutics with multimodal imaging properties.
Citace poskytuje Crossref.org
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- $a Jirátová, Markéta, $d 1990- $7 xx0252774 $u Institute for Clinical and Experimental Medicine (IKEM), Videnska 1958/9, 140 21, Prague 4, Czech Republic. Department of Physiology, Faculty of Science, Charles University in Prague, Viničná 7, 128 44, Praha 2, Czech Republic.
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- $a Biological characterization of a novel hybrid copolymer carrier system based on glycogen / $c M. Jirátová, A. Pospíšilová, M. Rabyk, M. Pařízek, J. Kovář, A. Gálisová, M. Hrubý, D. Jirák,
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- $a The effective drug delivery systems for cancer treatment are currently on high demand. In this paper, biological behavior of the novel hybrid copolymers based on polysaccharide glycogen were characterized. The copolymers were modified by fluorescent dyes for flow cytometry, confocal microscopy, and in vivo fluorescence imaging. Moreover, the effect of oxazoline grafts on degradation rate was examined. Intracellular localization, cytotoxicity, and internalization route of the modified copolymers were examined on HepG2 cell line. Biodistribution of copolymers was addressed by in vivo fluorescence imaging in C57BL/6 mice. Our results indicate biocompatibility, biodegradability, and non-toxicity of the glycogen-based hybrid copolymers. Copolymers were endocyted into the cytoplasm, most probably via caveolae-mediated endocytosis. Higher content of oxazoline in polymers slowed down cellular uptake. No strong colocalization of the glycogen-based probe with lysosomes was observed; thus, it seems that the modified externally administered glycogen is degraded in the same way as an endogenous glycogen. In vivo experiment showed relatively fast biodistribution and biodegradation. In conclusion, this novel nanoprobe offers unique chemical and biological attributes for its use as a novel drug delivery system that might serve as an efficient carrier for cancer therapeutics with multimodal imaging properties.
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