Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

The role of the microbiome and psychosocial stress in the expression and activity of drug metabolizing enzymes in mice

N. Zemanová, P. Anzenbacher, I. Zapletalová, L. Jourová, P. Hermanová, T. Hudcovic, H. Kozáková, M. Vodička, J. Pácha, E. Anzenbacherová,

. 2020 ; 10 (1) : 8529. [pub] 20200522

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc20028162

The gut microbiota is involved in a number of different metabolic processes of the host organism, including the metabolism of xenobiotics. In our study, we focused on liver cytochromes P450 (CYPs), which can metabolize a wide range of exo- and endogenous molecules. We studied changes in mRNA expression and CYP enzyme activities, as well as the mRNA expression of transcription factors that have an important role in CYP expression, all in stressed germ-free (GF) and stressed specific-pathogen-free (SPF) mice. Besides the presence of the gut microbiota, we looked at the difference between acute and chronic stress. Our results show that stress has an impact on CYP mRNA expression, but it is mainly chronic stress that has a significant effect on enzyme activities along with the gut microbiome. In acutely stressed mice, we observed significant changes at the mRNA level, however, the corresponding enzyme activities were not influenced. Our study suggests an important role of the gut microbiota along with chronic psychosocial stress in the expression and activity of CYPs, which can potentially lead to less effective drug metabolism and, as a result, a harmful impact on the organism.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20028162
003      
CZ-PrNML
005      
20211208102151.0
007      
ta
008      
210105s2020 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41598-020-65595-9 $2 doi
035    __
$a (PubMed)32444678
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Zemanová, Nina $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic. $7 xx0267214
245    14
$a The role of the microbiome and psychosocial stress in the expression and activity of drug metabolizing enzymes in mice / $c N. Zemanová, P. Anzenbacher, I. Zapletalová, L. Jourová, P. Hermanová, T. Hudcovic, H. Kozáková, M. Vodička, J. Pácha, E. Anzenbacherová,
520    9_
$a The gut microbiota is involved in a number of different metabolic processes of the host organism, including the metabolism of xenobiotics. In our study, we focused on liver cytochromes P450 (CYPs), which can metabolize a wide range of exo- and endogenous molecules. We studied changes in mRNA expression and CYP enzyme activities, as well as the mRNA expression of transcription factors that have an important role in CYP expression, all in stressed germ-free (GF) and stressed specific-pathogen-free (SPF) mice. Besides the presence of the gut microbiota, we looked at the difference between acute and chronic stress. Our results show that stress has an impact on CYP mRNA expression, but it is mainly chronic stress that has a significant effect on enzyme activities along with the gut microbiome. In acutely stressed mice, we observed significant changes at the mRNA level, however, the corresponding enzyme activities were not influenced. Our study suggests an important role of the gut microbiota along with chronic psychosocial stress in the expression and activity of CYPs, which can potentially lead to less effective drug metabolism and, as a result, a harmful impact on the organism.
650    _2
$a zvířata $7 D000818
650    _2
$a systém (enzymů) cytochromů P-450 $x genetika $x metabolismus $7 D003577
650    _2
$a střevní mikroflóra $x fyziologie $7 D000069196
650    _2
$a regulace genové exprese enzymů $7 D015971
650    _2
$a játra $x enzymologie $x mikrobiologie $7 D008099
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a myši $7 D051379
650    _2
$a myši inbrední BALB C $7 D008807
650    _2
$a messenger RNA $x genetika $x metabolismus $7 D012333
650    12
$a psychický stres $7 D013315
650    _2
$a transkripční faktory $x genetika $x metabolismus $7 D014157
650    _2
$a xenobiotika $x metabolismus $7 D015262
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Anzenbacher, Pavel $u Department of Pharmacology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
700    1_
$a Zapletalová, Iveta $u Department of Pharmacology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic. $7 xx0267200
700    1_
$a Jourová, Lenka $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic. LenkaJourova@seznam.cz. $7 xx0267202
700    1_
$a Hermanová, Petra $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Nový Hrádek, Czech Republic. $7 xx0267221
700    1_
$a Hudcovic, Tomáš, $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Nový Hrádek, Czech Republic. $d 1965- $7 xx0267225
700    1_
$a Kozáková, Hana $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Nový Hrádek, Czech Republic.
700    1_
$a Vodička, Martin $u Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Pácha, Jiří $u Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Anzenbacherová, Eva $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic.
773    0_
$w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 10, č. 1 (2020), s. 8529
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32444678 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20210105 $b ABA008
991    __
$a 20211208102149 $b ABA008
999    __
$a ok $b bmc $g 1608497 $s 1119342
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 10 $c 1 $d 8529 $e 20200522 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
LZP    __
$a Pubmed-20210105

Najít záznam

Citační ukazatele

Možnosti archivace