Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Comparison of methods used for evaluation of mutagenicity/genotoxicity of model chemicals - parabens

J. Chrz, B. Hošíková, L. Svobodová, D. Očadlíková, H. Kolářová, M. Dvořáková, K. Kejlová, L. Malina, G. Jírová, A. Vlková, M. Mannerström

. 2020 ; 69 (Suppl 4) : S661-S679. [pub] 20201231

Jazyk angličtina Země Česko

Typ dokumentu srovnávací studie, časopisecké články, přehledy

Perzistentní odkaz   https://www.medvik.cz/link/bmc21028113

Growing worldwide efforts to replace (reduce) animal testing and to improve alternative in vitro tests which may be more efficient in terms of both time, cost and scientific validity include also genotoxicity/mutagenicity endpoints. The aim of the review article was to summarize currently available in vitro testing approaches in this field, their regulatory acceptance and recommended combinations for classification of chemicals. A study using the combination of Comet Assay performed on two cell lines and the Chromosomal Aberration test on human peripheral lymphocytes was performed with the aim to predict the genotoxic potential of selected paraben esters, serving as a model chemical group. Parabens are widely used in consumer products as preservatives and have been reported to exhibit inconclusive results in numerous genotoxicity studies. The Comet Assay identified Ethylparaben and Benzylparaben as potentially genotoxic. The Chromosomal Aberration test revealed weak genotoxic potential in case of Ethylparaben and positive genotoxicity in case of Butylparaben, Propylparaben and Isopropylparaben. The main reasons for variability seem to be limited water solubility of parabens, determining their bioavailability at the cellular level, and absence of metabolic activation in the Comet Assay. The results confirmed that the Comet Assay should serve as a screening test and should not be used as a stand-alone method for classification of genotoxicity. The weight of evidence approach in risk assessment should be supported with data generated with the use of human relevant in vitro methods based on cells / tissues of human origin.

Citace poskytuje Crossref.org

Bibliografie atd.

Literatura

000      
00000naa a2200000 a 4500
001      
bmc21028113
003      
CZ-PrNML
005      
20211208163736.0
007      
ta
008      
211105s2020 xr d f 000 0|eng||
009      
AR
024    7_
$a 10.33549/physiolres.934615 $2 doi
035    __
$a (PubMed)33646007
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Chrz, Jan, $d 1986- $7 xx0267257 $u National Institute of Public Health, Centre of Toxicology and Health Safety, Prague, Czech Republic $u Department of Medical Biophysics, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Czech Republic
245    10
$a Comparison of methods used for evaluation of mutagenicity/genotoxicity of model chemicals - parabens / $c J. Chrz, B. Hošíková, L. Svobodová, D. Očadlíková, H. Kolářová, M. Dvořáková, K. Kejlová, L. Malina, G. Jírová, A. Vlková, M. Mannerström
504    __
$a Literatura
520    9_
$a Growing worldwide efforts to replace (reduce) animal testing and to improve alternative in vitro tests which may be more efficient in terms of both time, cost and scientific validity include also genotoxicity/mutagenicity endpoints. The aim of the review article was to summarize currently available in vitro testing approaches in this field, their regulatory acceptance and recommended combinations for classification of chemicals. A study using the combination of Comet Assay performed on two cell lines and the Chromosomal Aberration test on human peripheral lymphocytes was performed with the aim to predict the genotoxic potential of selected paraben esters, serving as a model chemical group. Parabens are widely used in consumer products as preservatives and have been reported to exhibit inconclusive results in numerous genotoxicity studies. The Comet Assay identified Ethylparaben and Benzylparaben as potentially genotoxic. The Chromosomal Aberration test revealed weak genotoxic potential in case of Ethylparaben and positive genotoxicity in case of Butylparaben, Propylparaben and Isopropylparaben. The main reasons for variability seem to be limited water solubility of parabens, determining their bioavailability at the cellular level, and absence of metabolic activation in the Comet Assay. The results confirmed that the Comet Assay should serve as a screening test and should not be used as a stand-alone method for classification of genotoxicity. The weight of evidence approach in risk assessment should be supported with data generated with the use of human relevant in vitro methods based on cells / tissues of human origin.
650    12
$a alternativy testů na zvířatech $7 D000826
650    _2
$a zvířata $7 D000818
650    _2
$a viabilita buněk $x účinky léků $7 D002470
650    _2
$a chromozomální aberace $x chemicky indukované $7 D002869
650    _2
$a kometový test $7 D020552
650    12
$a poškození DNA $7 D004249
650    _2
$a buněčné linie keratinocytů HaCaT $7 D000084282
650    _2
$a lidé $7 D006801
650    _2
$a lymfocyty $x účinky léků $x patologie $7 D008214
650    _2
$a mikrojádra chromozomálně defektní $x chemicky indukované $7 D048629
650    _2
$a mikrojaderné testy $7 D015162
650    _2
$a mutageneze $x účinky léků $7 D016296
650    12
$a testy genotoxicity $7 D009152
650    _2
$a parabeny $x toxicita $7 D010226
650    _2
$a hodnocení rizik $7 D018570
655    _2
$a srovnávací studie $7 D003160
655    _2
$a časopisecké články $7 D016428
655    _2
$a přehledy $7 D016454
700    1_
$a Hošíková, Barbora $7 xx0244654 $u Department of Medical Biophysics, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Czech Republic
700    1_
$a Svobodová, Lada $7 xx0267259 $u National Institute of Public Health, Centre of Toxicology and Health Safety, Prague, Czech Republic $u Department of Medical Biophysics, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Czech Republic
700    1_
$a Očadlíková, Danuše $7 xx0068934 $u National Institute of Public Health, Centre of Toxicology and Health Safety, Prague, Czech Republic
700    1_
$a Kolářová, Hana, $d 1961- $7 _gn020022061 $u Department of Medical Biophysics, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Czech Republic
700    1_
$a Dvořáková, Markéta, $d 1977 leden 17.- $7 xx0267267 $u National Institute of Public Health, Centre of Toxicology and Health Safety, Prague, Czech Republic
700    1_
$a Kejlová, Kristina $7 xx0160869 $u National Institute of Public Health, Centre of Toxicology and Health Safety, Prague, Czech Republic
700    1_
$a Malina, Lukáš, $d 1988- $7 xx0244734 $u Department of Medical Biophysics, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Czech Republic
700    1_
$a Jírová, Gabriela $7 xx0267256 $u National Institute of Public Health, Centre of Toxicology and Health Safety, Prague, Czech Republic $u Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Czech Republic
700    1_
$a Vlková, Alena $7 xx0241137 $u National Institute of Public Health, Centre of Toxicology and Health Safety, Prague, Czech Republic $u Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Czech Republic
700    1_
$a Mannerström, M. $u FICAM, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland
773    0_
$w MED00003824 $t Physiological research $x 1802-9973 $g Roč. 69, Suppl 4 (2020), s. S661-S679
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33646007 $y Pubmed
910    __
$a ABA008 $b A 4120 $c 266 $y p $z 0
990    __
$a 20211105 $b ABA008
991    __
$a 20211208163735 $b ABA008
999    __
$a ok $b bmc $g 1728726 $s 1148658
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 69 $c Suppl 4 $d S661-S679 $e 20201231 $i 1802-9973 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
LZP    __
$b NLK118 $a Pubmed-20211105

Najít záznam

Citační ukazatele

Možnosti archivace