• Je něco špatně v tomto záznamu ?

Detection of prions in matching post-mortem skin and cerebrospinal fluid samples using second-generation real-time quaking-induced conversion assay

S. Baranová, T. Moško, M. Brůžová, T. Haldiman, C. Kim, JG. Safar, R. Matěj, K. Holada

. 2024 ; 14 (1) : 6294. [pub] 20240315

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc24006716

Grantová podpora
R01 NS074317 NINDS NIH HHS - United States

Real-time quaking-induced conversion assay (RT-QuIC) exploits templating activity of pathogenic prion protein for ultrasensitive detection of prions. We have utilized second generation RT-QuIC assay to analyze matching post-mortem cerebrospinal fluid and skin samples of 38 prion disease patients and of 30 deceased neurological controls. The analysis of cerebrospinal fluid samples led to 100% sensitivity and 100% specificity, but some samples had to be diluted before the analysis to alleviate the effect of present RT-QuIC inhibitors. The analysis of the corresponding skin samples provided 89.5% sensitivity and 100% specificity. The median seeding dose present in the skin was one order of magnitude higher than in the cerebrospinal fluid, despite the overall fluorescent signal of the skin samples was comparatively lower. Our data support the use of post-mortem cerebrospinal fluid for confirmation of prion disease diagnosis and encourage further studies of the potential of skin biopsy samples for intra-vitam prion diseases ́ diagnostics.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24006716
003      
CZ-PrNML
005      
20240516115717.0
007      
ta
008      
240412s2024 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41598-024-56789-6 $2 doi
035    __
$a (PubMed)38491063
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Baranová, Soňa $u Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University, Prague, Czech Republic $7 xx0317357
245    10
$a Detection of prions in matching post-mortem skin and cerebrospinal fluid samples using second-generation real-time quaking-induced conversion assay / $c S. Baranová, T. Moško, M. Brůžová, T. Haldiman, C. Kim, JG. Safar, R. Matěj, K. Holada
520    9_
$a Real-time quaking-induced conversion assay (RT-QuIC) exploits templating activity of pathogenic prion protein for ultrasensitive detection of prions. We have utilized second generation RT-QuIC assay to analyze matching post-mortem cerebrospinal fluid and skin samples of 38 prion disease patients and of 30 deceased neurological controls. The analysis of cerebrospinal fluid samples led to 100% sensitivity and 100% specificity, but some samples had to be diluted before the analysis to alleviate the effect of present RT-QuIC inhibitors. The analysis of the corresponding skin samples provided 89.5% sensitivity and 100% specificity. The median seeding dose present in the skin was one order of magnitude higher than in the cerebrospinal fluid, despite the overall fluorescent signal of the skin samples was comparatively lower. Our data support the use of post-mortem cerebrospinal fluid for confirmation of prion disease diagnosis and encourage further studies of the potential of skin biopsy samples for intra-vitam prion diseases ́ diagnostics.
650    _2
$a lidé $7 D006801
650    12
$a priony $x metabolismus $7 D011328
650    12
$a prionové nemoci $x diagnóza $7 D017096
650    _2
$a kůže $x metabolismus $7 D012867
650    _2
$a prionová bílkovina $7 D000072002
650    _2
$a biotest $7 D001681
650    12
$a Creutzfeldtova-Jakobova nemoc $x diagnóza $x mozkomíšní mok $7 D007562
655    _2
$a časopisecké články $7 D016428
700    1_
$a Moško, Tibor $u Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Brůžová, Magdalena $u Department of Pathology and Molecular Medicine, Third Faculty of Medicine, Charles University and Thomayer University Hospital, Prague, Czech Republic
700    1_
$a Haldiman, Tracy $u Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH, 44106, USA $u Department Neurology, Case Western Reserve University School of Medicine, Cleveland, OH, 44106, USA
700    1_
$a Kim, Chae $u Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH, 44106, USA $u Department Neurology, Case Western Reserve University School of Medicine, Cleveland, OH, 44106, USA
700    1_
$a Safar, Jiri G $u Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH, 44106, USA $u Department Neurology, Case Western Reserve University School of Medicine, Cleveland, OH, 44106, USA
700    1_
$a Matěj, Radoslav $u Department of Pathology and Molecular Medicine, Third Faculty of Medicine, Charles University and Thomayer University Hospital, Prague, Czech Republic $u Department of Pathology, Third Faculty of Medicine, Charles University and University Hospital Kralovske Vinohrady, Prague, Czech Republic $u Department of Pathology, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic
700    1_
$a Holada, Karel $u Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University, Prague, Czech Republic. karel.holada@lf1.cuni.cz
773    0_
$w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 14, č. 1 (2024), s. 6294
856    41
$u https://pubmed.ncbi.nlm.nih.gov/38491063 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20240412 $b ABA008
991    __
$a 20240516115711 $b ABA008
999    __
$a ok $b bmc $g 2080985 $s 1216483
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 14 $c 1 $d 6294 $e 20240315 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
GRA    __
$a R01 NS074317 $p NINDS NIH HHS $2 United States
LZP    __
$a Pubmed-20240412

Najít záznam

Citační ukazatele

Možnosti archivace