• Je něco špatně v tomto záznamu ?

Transgenic rat with ubiquitous expression of angiotensin-(1-7)-producing fusion protein: a new tool to study the role of protective arm of the renin-angiotensin system in the pathophysiology of cardio-renal diseases

L. Červenka, Z. Husková, S. Kikerlová, O. Gawrys, Š. Vacková, P. Škaroupková, J. Sadowski, M. Miklovič, M. Molnár, M. Táborský, V. Melenovský, M. Bader

. 2025 ; 48 (1) : 336-352. [pub] 20241113

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc25010473

The aim of the present study was to assess systemic circulatory and tissue activities of both the classical arm and of the alternative arm of the renin-angiotensin system (RAS) in a new transgenic rat line (TG7371) that expresses angiotensin-(1-7) (ANG 1-7)-producing fusion protein; the results were compared with the activities measured in control transgene-negative Hannover Sprague-Dawley (HanSD) rats. Plasma and tissue concentrations of angiotensin II (ANG II) and ANG 1-7, and kidney mRNA expressions of receptors responsible for biological actions of ANG II and ANG 1-7 [i.e. ANG II type 1 and type 2 (AT1 and AT2) and Mas receptors] were assessed in TG7371 transgene-positive and in HanSD rats. We found that male TG7371 transgene-positive rats exhibited significantly elevated plasma, kidney, heart and lung ANG 1-7 concentrations as compared with control male HanSD rats; by contrast, there was no significant difference in ANG II concentrations and no significant differences in mRNA expression of AT1, AT2 and Mas receptors. In addition, we found that in male TG7371 transgene-positive rats blood pressure was lower than in male HanSD rats. These data indicate that the balance between the classical arm and the alternative arm of the RAS was in male TGR7371 transgene-positive rats markedly shifted in favor of the latter. In conclusion, TG7371 transgene-positive rats represent a new powerful tool to study the long-term role of the alternative arm of the RAS in the pathophysiology and potentially in the treatment of cardio-renal diseases.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25010473
003      
CZ-PrNML
005      
20250429134904.0
007      
ta
008      
250415s2025 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41440-024-01995-y $2 doi
035    __
$a (PubMed)39537982
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Červenka, Luděk $u Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. luce@ikem.cz $u Department of Internal Medicine I, Cardiology, University Hospital Olomouc and Palacký University, Olomouc, Czech Republic. luce@ikem.cz
245    10
$a Transgenic rat with ubiquitous expression of angiotensin-(1-7)-producing fusion protein: a new tool to study the role of protective arm of the renin-angiotensin system in the pathophysiology of cardio-renal diseases / $c L. Červenka, Z. Husková, S. Kikerlová, O. Gawrys, Š. Vacková, P. Škaroupková, J. Sadowski, M. Miklovič, M. Molnár, M. Táborský, V. Melenovský, M. Bader
520    9_
$a The aim of the present study was to assess systemic circulatory and tissue activities of both the classical arm and of the alternative arm of the renin-angiotensin system (RAS) in a new transgenic rat line (TG7371) that expresses angiotensin-(1-7) (ANG 1-7)-producing fusion protein; the results were compared with the activities measured in control transgene-negative Hannover Sprague-Dawley (HanSD) rats. Plasma and tissue concentrations of angiotensin II (ANG II) and ANG 1-7, and kidney mRNA expressions of receptors responsible for biological actions of ANG II and ANG 1-7 [i.e. ANG II type 1 and type 2 (AT1 and AT2) and Mas receptors] were assessed in TG7371 transgene-positive and in HanSD rats. We found that male TG7371 transgene-positive rats exhibited significantly elevated plasma, kidney, heart and lung ANG 1-7 concentrations as compared with control male HanSD rats; by contrast, there was no significant difference in ANG II concentrations and no significant differences in mRNA expression of AT1, AT2 and Mas receptors. In addition, we found that in male TG7371 transgene-positive rats blood pressure was lower than in male HanSD rats. These data indicate that the balance between the classical arm and the alternative arm of the RAS was in male TGR7371 transgene-positive rats markedly shifted in favor of the latter. In conclusion, TG7371 transgene-positive rats represent a new powerful tool to study the long-term role of the alternative arm of the RAS in the pathophysiology and potentially in the treatment of cardio-renal diseases.
650    _2
$a zvířata $7 D000818
650    12
$a angiotensin I $x metabolismus $7 D000803
650    12
$a renin-angiotensin systém $x fyziologie $7 D012084
650    12
$a potkani transgenní $7 D055647
650    12
$a peptidové fragmenty $x metabolismus $7 D010446
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a krysa rodu Rattus $7 D051381
650    12
$a angiotensin II $7 D000804
650    12
$a potkani Sprague-Dawley $7 D017207
650    _2
$a ledviny $x metabolismus $7 D007668
650    _2
$a protoonkogen Mas $7 D000090063
650    _2
$a receptory spřažené s G-proteiny $x genetika $x metabolismus $7 D043562
650    _2
$a receptor angiotensinu typ 1 $x genetika $x metabolismus $7 D044140
650    _2
$a nemoci ledvin $x metabolismus $x genetika $7 D007674
650    _2
$a rekombinantní fúzní proteiny $x metabolismus $7 D011993
650    _2
$a kardiovaskulární nemoci $x metabolismus $x genetika $7 D002318
650    _2
$a krevní tlak $x fyziologie $7 D001794
655    _2
$a časopisecké články $7 D016428
700    1_
$a Husková, Zuzana $u Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
700    1_
$a Kikerlová, Soňa $u Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
700    1_
$a Gawrys, Olga $u Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
700    1_
$a Vacková, Šárka $u Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic $u Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic
700    1_
$a Škaroupková, Petra $u Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
700    1_
$a Sadowski, Janusz $u Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
700    1_
$a Miklovič, Matúš $u Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic $u Department of Pathophysiology, Second Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Molnár, Matej $u Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic $u Department of Pathophysiology, Second Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Táborský, Miloš $u Department of Internal Medicine I, Cardiology, University Hospital Olomouc and Palacký University, Olomouc, Czech Republic
700    1_
$a Melenovský, Vojtěch $u Department of Cardiology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
700    1_
$a Bader, Michael $u Max-Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany $u DZHK (German Center for Cardiovascular Research), Partner Site Berlin, Berlin, Germany $u Institute for Biology, University of Lübeck, Lübeck, Germany $u Charité University Medicine Berlin, Berlin, Germany
773    0_
$w MED00006041 $t Hypertension research $x 1348-4214 $g Roč. 48, č. 1 (2025), s. 336-352
856    41
$u https://pubmed.ncbi.nlm.nih.gov/39537982 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250415 $b ABA008
991    __
$a 20250429134900 $b ABA008
999    __
$a ok $b bmc $g 2311683 $s 1247554
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2025 $b 48 $c 1 $d 336-352 $e 20241113 $i 1348-4214 $m Hypertension research $n Hypertens Res $x MED00006041
LZP    __
$a Pubmed-20250415

Najít záznam

Citační ukazatele

Možnosti archivace