Capillary gas chromatography of underivatized fatty acids with a free fatty acid phase column and a programmed temperature vaporizer injector
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články
- MeSH
- analýza potravin MeSH
- chromatografie plynová MeSH
- fosfatidylcholiny krev MeSH
- kyseliny mastné neesterifikované chemie MeSH
- lidé MeSH
- mastné kyseliny analýza krev chemie MeSH
- teplota MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fosfatidylcholiny MeSH
- kyseliny mastné neesterifikované MeSH
- mastné kyseliny MeSH
Underivatized fatty acids occurring in lipids of a number of biological specimens (blood plasma, tissues, food) were studied using a capillary column with a chemically bonded free fatty acid phase and a programmed temperature vaporizer. Non-linear calibration dependences were obtained for almost all the acids at lower concentrations because of losses in the column, non-linearity increasing with increasing carbon number and degree of unsaturation. Sample introduction at temperatures below the solvent boiling point eliminates losses in the injector. The lower recovery of long-chain polyunsaturated acids is caused by interactions occurring in the column. Some critical pairs remain unresolved in the underivatized form (18:1n9-18:1n7, 20:4n6-20:3n3), whereas the corresponding methyl esters exhibit baseline resolution.
Citace poskytuje Crossref.org
