Identification and characterization of natural antibodies against tau protein in an intravenous immunoglobulin product
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26616881
DOI
10.1016/j.jneuroim.2015.10.017
PII: S0165-5728(15)30063-1
Knihovny.cz E-zdroje
- Klíčová slova
- Alzheimer disease, Avidity, Immunoreactivity, Intravenous immunoglobulins, Natural antibodies, Tau protein,
- MeSH
- epitopy chemie MeSH
- imunoglobulin G imunologie izolace a purifikace MeSH
- imunosorpční techniky MeSH
- intravenózní imunoglobuliny chemie imunologie MeSH
- lidé MeSH
- molekulová hmotnost MeSH
- peptidy imunologie MeSH
- proteiny tau imunologie MeSH
- terciární struktura proteinů MeSH
- vazba proteinů imunologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- epitopy MeSH
- imunoglobulin G MeSH
- intravenózní imunoglobuliny MeSH
- peptidy MeSH
- proteiny tau MeSH
The latest therapeutic approaches to Alzheimer disease are using intravenous immunoglobulin (IVIG) products. Therefore, the detailed characterization of target-specific antibodies naturally occurring in IVIG products is beneficial. We have focused on characterization of antibodies isolated against tau protein, a biomarker of Alzheimer's disease, from Flebogamma IVIG product. The analysis of IgG subclass distribution indicated skewing toward IgG3 in anti-tau-enriched IgG fraction. The evaluation of their reactivity and avidity with several recombinant tau forms was performed by ELISA and blotting techniques. Truncated non-phosphorylated tau protein (amino acids 155-421) demonstrated the highest reactivity and avidity index. We provide the first detailed insight into the reactivity of isolated natural antibodies against tau protein.
Department of Biological and Biochemical Sciences University of Pardubice Pardubice Czech Republic
Department of Neurobiology AD Center National Institute of Mental Health Klecany Czech Republic
Citace poskytuje Crossref.org
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