Contribution of in vitro comparison of colorectal carcinoma cells from primary and metastatic lesions to elucidation of mechanisms of tumor progression and response to anticancer therapy
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články
PubMed
26790446
DOI
10.1007/s13277-016-4839-y
PII: 10.1007/s13277-016-4839-y
Knihovny.cz E-zdroje
- Klíčová slova
- Anticancer therapy, Colorectal carcinoma, Lymph node metastasis, Tumor progression, microRNA,
- MeSH
- buněčná adheze genetika MeSH
- kolorektální nádory farmakoterapie genetika patologie MeSH
- lidé MeSH
- lymfatické metastázy patologie MeSH
- lymfatické uzliny patologie MeSH
- matrixová metaloproteinasa 2 genetika MeSH
- matrixová metaloproteinasa 9 genetika MeSH
- mikro RNA genetika MeSH
- molekuly buněčné adheze genetika MeSH
- nádorové buněčné linie MeSH
- pohyb buněk genetika MeSH
- progrese nemoci MeSH
- protinádorové látky farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- matrixová metaloproteinasa 2 MeSH
- matrixová metaloproteinasa 9 MeSH
- mikro RNA MeSH
- molekuly buněčné adheze MeSH
- protinádorové látky MeSH
Colorectal cancer has been a leading cause of cancer-related morbidity and mortality. For the research and individualization of therapy, primary cell lines of the colorectal cancer appear to be still an invaluable tool. We evaluated the differences in metastatic potential between four isolated primary colon cancer cells and cells derived from their lymph node metastasis. These results were compared with correspond immortalized cells-SW480 and SW620, respectively. The ability to migrate was tested using real-time measurement in xCELLigence system. Expressions of molecules involved in adhesion and invasion processes were examined using RT-PCR and western blot analysis. Furthermore, impact of cytotoxic effect of selected chemotherapeutics (irinotecan, oxaliplatin) and biological therapy (bevacizumab, cetuximab, panitumumab) was assessed by the WST assay. As expected, cell lines derived from lymph node migrated more aggressively and higher expression of adhesion molecules ICAM-1, EpCAM, and N-cadherin was detected. The expression of MMP-2 and -9 was elevated, on the other hand, in cell lines derived from primary tumor cancer cells as well as the expression of miR-21, miR-29a, and miR-200a. The most pronounced cytotoxic effect has been recorded with oxaliplatin and irinotecan (IC50 = 48.23 resp. 0.11 μg/ml), especially in cells originating from lymph node metastases. In total, comparison of isolated cell lines and immortalized cell lines has shown many similarities, as well as several differences. Adhesion/invasion molecules and several miRNAs, which play an important role in tumor development and the invasive and migratory behavior, could be a useful therapeutic target in malignant colorectal cancer.
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