Three polysaccharide complexes (PSCs) were isolated from the aerial parts of common purslane (Portulaca oleracea L.), and the flowers of common lavender (Lavandula angustifolia Mill.) and silver linden (Tilia tomentosa Moench) by boiling water extraction and ethanol precipitation. The chemical composition and immunomodulating effects of isolated PSCs were characterized. The chemical characterization revealed that the three samples contain mainly pectic polysaccharides. They exhibited ex vivo intestinal immunomodulating activity through the murine Peyer's patch-mediated bone marrow cell proliferation test at 100μg/ml concentration. At the same time, they stimulated ex vivo human blood T-cell populations (CD4+/CD25+ and CD8+/CD25+), phagocytic leukocytes (CD14+ and CD64+ cells) and induced IL-6 production from human white blood cells and Peyer's patch cells. The herbal PSCs stimulated ex vivo ROS production from whole blood phagocytes and showed unspecific in vitro anti-proliferative activity against normal and A549, HeLa and LS180 tumor cells. This is the first report on immunomodulating studies of linden flower pectins and chemical and biological activity characterization of lavender polysaccharides. Our study demonstrates that similarly to purslane, lavender and silver linden herbal materials contain immunomodulating polysaccharides that could be useful for support of compromised immune system.

Department of Developmental Biology Plovdiv University Paisii Hilendarski 24 Tsar Assen Str 4000 Plovdiv Bulgaria

Department of Drug Discovery Science Kitasato Institute for Life Sciences Kitasato University 5 9 1 Shirokane Minato ku 108 8641 Tokyo Japan

Department of Free Radical Pathophysiology Institute of Biophysics Czech Academy of Sciences 135 Kralovopolska 612 65 Brno Czech Republic

Department of Pharmaceutical Chemistry School of Pharmacy University of Oslo P O Box 1068 Blindern 0316 Oslo Norway

Laboratory of Biologically Active Substances Institute of Organic Chemistry with Centre of Phytochemistry Bulgarian Academy of Sciences 139 Ruski Blvd 4000 Plovdiv Bulgaria; Innovative Technological Center Ltd 20 Dr G M Dimitrov Str 4000 Plovdiv Bulgaria

Laboratory of Experimental Chromatography and Mass Spectrometry Institute of Organic Chemistry with Centre of Phytochemistry Bulgarian Academy of Sciences Acad G Bonchev Str Bl 9 1113 Sofia Bulgaria

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