Association of the combined parameters including the frequency of primary cilia, PD-L1, Smoothened protein, membranous β-catenin and cytoplasmic β-catenin expression with the outcome of patients with clear cell renal cell carcinoma
Jazyk angličtina Země Česko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
38420990
DOI
10.5507/bp.2024.005
Knihovny.cz E-zdroje
- Klíčová slova
- clear cell renal carcinoma, cytoplasmic β-catenin, membranous β-catenin, primary cilia, programmed cell death protein ligand 1, smoothened protein,
- MeSH
- antigeny CD274 * metabolismus MeSH
- beta-katenin * metabolismus MeSH
- cilie * metabolismus patologie MeSH
- dospělí MeSH
- imunohistochemie MeSH
- karcinom z renálních buněk * metabolismus mortalita patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádorové biomarkery metabolismus MeSH
- nádory ledvin * metabolismus mortalita patologie MeSH
- prognóza MeSH
- receptor Smoothened metabolismus MeSH
- receptory spřažené s G-proteiny * metabolismus MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antigeny CD274 * MeSH
- beta-katenin * MeSH
- CD274 protein, human MeSH Prohlížeč
- CTNNB1 protein, human MeSH Prohlížeč
- nádorové biomarkery MeSH
- receptor Smoothened MeSH
- receptory spřažené s G-proteiny * MeSH
- SMO protein, human MeSH Prohlížeč
AIMS: The objective of this study was to investigate the association and combined prognostic significance of the PD-L1, Smoothened protein and β-catenin expressions in patients with clear cell renal cell carcinoma (ccRCC). METHODS: The PD-L1, Smoothened protein and β-catenin expression were evaluated in 104 ccRCC patients. All studied tumor samples were acquired from nephrectomy specimens of primary tumors and not from biopsies or metastases. An indirect immunohistochemistry using polyclonal rabbit anti-Smoothened antibody, monoclonal mouse anti-human β-catenin-1 antibody, immunohistochemical assay PD-L1 28-8 pharmDx using monoclonal rabbit anti-PD-L1 antibody and anti-VHL (C- terminal) rabbit antibody was used. Immunohistochemistry was scored semiquantitavely. RESULTS: Median overall survival (OS) was significantly better in patients with lower PD-L1 expression (≤5%), Smoothened protein (SMO) expression (<5%) or cytoplasmic β-catenin expression (≤75%) than in patients with higher expressions of these biomarkers (P<0.001, P=0.047, and P<0.001, respectively). Membranous β-catenin showed an opposite effect with its lower expression (≤75%) being associated with longer OS (P=0.020). There was significant association between PD-1 and PD-L1 expression (P=0.007) and significant association of tumor grade (WHO 2016) with membranous β-catenin (P<0.001), cytoplasmic β-catenin (P=0.005), pVHL (P=0.042), PD-L1 (P=0.049) and PD-1 (P=0.028) expression. CONCLUSION: The present study provides the first data on the potential association and combined prognostic significance of frequency of primary cilia, PD-L1, Smoothened protein and β-catenin expression with the outcome in clear cell renal cell carcinoma.
Department of Oncology Regional Hospital Liberec Czech Republic
Department of Pathology Regional Hospital Kolin Czech Republic
Institute of Biostatistics and Analyses Faculty of Medicine Masaryk University Brno Czech Republic
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