Haematological parameters, plasma iron concentration, and bodyweight were monitored in Melanoma-bearing Libechov Minipigs (MeLiM) from 5 to 18 weeks old. Animals with melanoma progression (P group) and spontaneous regression (SR group) were compared. The P group showed the lowest median values of red blood cell counts (RBC), haematocrit (HCT), haemoglobin concentration (HGB), and bodyweight, whereas the control white (tumour-free) pigs (C group) revealed the highest mean values of these parameters. The mean values of pigs with SR fell between the P and C groups. In addition, a stable concentration of plasma iron was found in the C group, while iron deficiency that increases with age was observed in the MeLiM groups. These results indicate that MeLiM are affected by cancer-related microcytic hypochromic anaemia. The lowest values of HGB, RBC, and HCT, together with the highest number of platelets (PLT) in the P group correspond to melanoma progression. Higher values of these parameters and lower PLT in the MeLiM pigs with SR reflected health improvement due to the destruction of melanoma cells during spontaneous regression. Monitoring of these haematological parameters can help distinguish MeLiM piglets with progression and spontaneous regression of melanoma in the early stages of postnatal development. The findings of this study correspond to findings in human patients in which cancer-related anaemia, thrombocytosis, and iron deficiency are often diagnosed.
- MeSH
- hematologické testy veterinární MeSH
- melanom krev patofyziologie veterinární MeSH
- miniaturní prasata MeSH
- nádory kůže krev patofyziologie veterinární MeSH
- nemoci prasat krev patofyziologie MeSH
- prasata MeSH
- progrese nemoci MeSH
- spontánní regrese nádoru MeSH
- tělesná hmotnost MeSH
- železo aplikace a dávkování krev MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The present study describes the distinct bone marrow (BM) and peripheral blood (PB) monocyte subpopulations detected by seven-colour flow cytometry. Mononuclear phagocytes were identified as viable CD172a(+) SWC8(-) CD203a(-) mononuclear leukocytes. After that, monocyte subpopulations were differentiated by using CD14, CD163 and SLA-DR markers. Four distinct monocyte subpopulations were found in the BM and PB. Based on the discovered populations two possible maturation pathways have been proposed. The first pathway was characterised by release of CD14(hi) CD163(-) SLA-DR(-) BM monocytes into the PB where they matured into CD14(low) CD163(+) SLA-DR(+) monocytes. In the alternative pathway the monocytes finalised their phenotypical maturation in the BM and then they were released into the PB as CD14(low) CD163(+) SLA-DR(+) cells. In Salmonella-infected piglets, the population of CD14(low) CD163(+) SLA-DR(+) monocytes was elevated in the BM and mesenteric lymph nodes (MLN), suggesting the role of this population in pathogenesis of Salmonella infection in pigs.
- MeSH
- buňky kostní dřeně klasifikace cytologie MeSH
- membránové proteiny MeSH
- monocyty klasifikace cytologie MeSH
- nemoci prasat krev mikrobiologie MeSH
- prasata MeSH
- průtoková cytometrie metody veterinární MeSH
- Salmonella enteritidis MeSH
- salmonelová infekce u zvířat krev MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Enterotoxigenic Escherichia coli (ETEC) is one of the most important causes of post-weaning diarrhea in piglets. Whilst serotype O149:F4 is frequently associated with hemorrhagic gastroenteritis, other serotypes have been found to be associated with mild or moderate enteritis. As neutrophils are recruited to sites of inflammation, the aim of this study was to ascertain whether or not there is any difference in the in vitro interaction between neutrophils and two different ETEC serotypes: O149:F4 and O147:F18. The association of bacteria with neutrophils was evaluated by flow cytometry. The respiratory burst was measured by the fluorescent probe dichlorofluorescein diacetate using flow cytometry and by L012-amplified chemiluminescence. The titers of antibodies against ETEC present in cultivation sera were assessed by agglutination. The viability of E. coli was ascertained by cultivation. It was found that the strains of O149 serotype were more frequently associated with neutrophils and induced a more intensive respiratory burst compared to the strains of O147 serotype. These differences might be due to the presence of different types of fimbriae on the surface of the strains tested and by the presence of anti-fimbrial antibodies in the porcine plasma. However, the intensive interaction between E. coli and the neutrophils and respiratory burst induced by the O149 strain did not lead to more efficient killing of the bacteria. It is suggested that a stronger respiratory burst may be an important factor causing severe clinical signs of post-weaning diarrhea in piglets.
- MeSH
- bakteriální fimbrie imunologie MeSH
- enterotoxigenní Escherichia coli imunologie MeSH
- infekce vyvolané Escherichia coli krev imunologie mikrobiologie veterinární MeSH
- nemoci prasat krev imunologie mikrobiologie MeSH
- neutrofily imunologie mikrobiologie MeSH
- prasata MeSH
- proteiny z Escherichia coli krev imunologie MeSH
- průjem krev imunologie mikrobiologie veterinární MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- Actinobacillus pleuropneumoniae imunologie MeSH
- bronchoalveolární lavážní tekutina imunologie MeSH
- ELISA veterinární MeSH
- financování organizované MeSH
- imunita získaná od matky MeSH
- imunoglobulin A biosyntéza MeSH
- infekce bakteriemi rodu Actinobacillus imunologie veterinární MeSH
- kolostrum imunologie MeSH
- lipopolysacharidy diagnostické užití MeSH
- nemoci prasat imunologie krev mikrobiologie MeSH
- novorozená zvířata MeSH
- protilátky bakteriální biosyntéza MeSH
- těhotenství MeSH
- výzkumný projekt MeSH
- zvířata MeSH
- Check Tag
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
