The ecotoxicological effect of after-usage released TiO2 nanoparticles in aquatic resources has been a major concern owing to their production and utilization in different applications. Addressing the issue, this study investigates the detailed in vivo molecular toxicity of TiO2 nanoparticles with Paramecium caudatum. TiO2 nanoparticles were synthesized at a lab scale using high energy ball milling technique; characterized for their physicochemical properties and investigated for their ecotoxicological impact on oxidative stress, steatosis, and apoptosis of cells through different biochemical analysis, flow cytometry, and fluorescent microscopy. TiO2 nanoparticles; TiO2 (N15); of size 36 ± 12 nm were synthesized with a zeta potential of - 20.2 ± 8.8 mV and bandgap of 4.6 ± 0.3 eV and exhibited a blue shift in UV-spectrum. Compared to the Bulk TiO2, the TiO2 (N15) exhibited higher cytotoxicity with a 24 h LC50 of 202.4 µg/ml with P. Caudatum. The mechanism was elucidated as the size and charge-dependent internalization of nanoparticles leading to abnormal physiological metabolism in oxidative stress, steatosis, and apoptosis because of their influential effect on the activity of metabolic proteins like SOD, GSH, MDA, and catalase. The study emphasized the controlled usage TiO2 nanoparticles in daily activity with a concern for ecological and biomedical aspects.
- Klíčová slova
- Ecotoxicity, Nanotoxicity, Oxidative stress, P. Caudatum, Steatosis, TiO(2) nanoparticles,
- MeSH
- apoptóza MeSH
- nanočástice * chemie toxicita MeSH
- oxidační stres MeSH
- Paramecium caudatum * MeSH
- titan toxicita MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- titan MeSH
- titanium dioxide MeSH Prohlížeč
Nitric oxide (NO) chemistry inside the body is the most interesting part of its behavior. NO is involved in controlling blood pressure, and in transmitting nerve signals and a variety of other signaling processes. To explain the behavior of NO, it is necessary to determine its immediate concentration or observe time-dependent changes in its concentration. In Paramecium caudatum, NO is formed by calcium-dependent nNOS (NOS1)-like protein, which is distributed in the cytoplasm. NO synthesis affects the ciliary beat and consequent motility of cells and blocked NO synthesis reduces the ability of cells to move. The possibility of online coupling of microdialysis (of P. caudatum solution) with NO detection is demonstrated. Direct measurement of NO is carried out using dilute Bluestar® Forensic reagent (luminol-H2 O2 system; one of the NO detections is based upon the chemiluminescent reaction between NO and the luminol-H2 O2 system, which is specifically reactive to NO). The effect of a nitric oxide synthase inhibitor, NG-nitro-l-arginine methyl ester was observed. NO production was inhibited and the movement of P. caudatum was restricted. These effects were time dependent and after a specific time were reversed.
- Klíčová slova
- Bluestar® Forensic, Paramecium caudatum, chemiluminescence, microdialysis, nitric oxide,
- MeSH
- inhibitory enzymů farmakologie MeSH
- luminiscenční měření MeSH
- luminol chemie MeSH
- mikrodialýza metody MeSH
- NG-nitroargininmethylester farmakologie MeSH
- oxid dusnatý analýza chemie metabolismus MeSH
- Paramecium caudatum chemie metabolismus MeSH
- peroxid vodíku chemie MeSH
- synthasa oxidu dusnatého antagonisté a inhibitory MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- inhibitory enzymů MeSH
- luminol MeSH
- NG-nitroargininmethylester MeSH
- oxid dusnatý MeSH
- peroxid vodíku MeSH
- synthasa oxidu dusnatého MeSH
- Klíčová slova
- COMPLEMENT *, EXPERIMENTAL LAB STUDY *, PARAMECIUM *, RADIATION INJURY, EXPERIMENTAL *, RATS *,
- MeSH
- experimentální radiační poranění * MeSH
- komplement * MeSH
- krysa rodu Rattus MeSH
- Paramecium caudatum * MeSH
- Paramecium * MeSH
- radiační poranění * MeSH
- výzkum * MeSH
- Check Tag
- krysa rodu Rattus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- komplement * MeSH
