T-lymphocyte subset
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The aim of the present study was to evaluate the biodosimetric potential of peripheral blood lymphocytes, particularly of T-cell subsets (null and T helper) and natural killer cells (NK), upon exposure to gamma irradiation (60Co) in vivo. For this purpose, the change in relative numbers of NK cells and T-lymphocyte subsets, as well as in the H2AX phosphorylation rate, were evaluated as potential early markers of the lymphocytic response to irradiation in vivo. These experiments were performed on a Large White Pig model. As a result, significant but not dose-dependent changes in the proportion of lymphocyte subpopulations (NK cells, null and T helper cells) were found after exposure to ionising radiation in vivo. On the other hand, circulating NK cells showed relatively higher radioresistance capacity when compared to the T-lymphocyte subsets; however, gamma-H2AX expression showed no significant difference between the evaluated lymphocyte subsets.
- MeSH
- buňky NK účinky záření MeSH
- fenotyp MeSH
- fosforylace MeSH
- histony metabolismus MeSH
- imunofenotypizace MeSH
- ionizující záření MeSH
- lymfocyty cytologie MeSH
- poškození DNA MeSH
- prasata MeSH
- radioizotopy kobaltu farmakologie MeSH
- radiometrie metody MeSH
- T-lymfocyty - podskupiny účinky záření MeSH
- záření gama MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- histony MeSH
- radioizotopy kobaltu MeSH
To objectivize possible immunomodulatory effects of polybacterial lysate Olimunostim (P. acnes, K. pneumoniae, S. aureus), splenic lymphocyte proliferation and T-lymphocyte subsets were followed-up in Balb/c mice administered perorally the lysate or saline (controls). The enhanced spontaneous lymphocyte proliferation observed at the beginning of Olimunostim application preceded a gradual increase of lymphocyte reactivity to T-mitogens reaching the maximum five days after the administration of the last dose and returning back to the control levels ten days afterwards. This stimulation of lymphocyte proliferation was accompanied by Olimunostim induced increase of CD4+ splenic lymphocytes being most pronounced five days after the end of immunomodulation and later returning to the initial values. The last experiment revealed an enhanced response of the in vivo primed lymphocytes after the re-exposure to Olimunostim in vitro. It is concluded that mostly nonspecific activation mechanisms, plausibly also parallelly induced specific immunity, are involved in Olimunostim modulatory effects on the cellular immune response.
- MeSH
- adjuvancia imunologická farmakologie MeSH
- aktivace lymfocytů účinky léků MeSH
- Klebsiella pneumoniae imunologie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- Propionibacterium acnes imunologie MeSH
- slezina imunologie MeSH
- Staphylococcus aureus imunologie MeSH
- T-lymfocyty - podskupiny účinky léků imunologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- adjuvancia imunologická MeSH
T-lymphocyte subsets (CD4/CD8 antigen positive cells) were determined in peripheral lymphocytes from 48 patients with breast cancer of different stages by flow immunocytometry with the aid of anti-CD4 and CD8 monoclonal antibodies. A broad individual variability of the CD4/CD8 ratio among both healthy donors and breast cancer patients was observed. The average value of CD4/CD8 ratio decreased in groups as follows: Healthy donors and Stage I patients, Stage IIA, IIB and Stage IV breast cancer patients. These differences were generally statistically not significant. The difference between healthy donors and Stage IV breast cancer patients was statistically significant (p < 0.01), if one exceedingly elevated value of the CD4/CD8 ratio was excluded from statistical evaluation. The average CD4/CD8 value in the group of breast cancer patients with lymph node or distant metastases was lower than that of patients without metastases, but their difference was not statistically significant either.
Bronchoalveolar T-lymphocyte subsets were evaluated in seven patients with pulmonary sarcoidosis and eight patients with other interstitial lung disorders. Both disease groups considerably differed in the distribution of bronchoalveolar CD4+ and CD8+ cells. The lymphocytic alveolitis in sarcoidosis was characterized by excess CD4+ (helper-inducer) cells; mean CD4/CD8 ratio was 6.8 +/- 3.8. Inversely, in the lungs of patients with other interstitial pulmonary disorders a less pronounced but clear predominance of CD8+ (suppressor-cytotoxic) lymphocytes was observed with CD4/CD8 ratio 0.7 +/- 0.4. The results of the study suggest that the determination of the major immunoregulatory subsets of bronchoalveolar lymphocytes contributes to the reliable differentiation of sarcoidosis from other interstitial lung disorders. The recent advances improving our understanding of the pathogenesis of sarcoidosis are briefly reviewed in the discussion.
- MeSH
- bronchoalveolární lavážní tekutina imunologie MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- plicní nemoci imunologie MeSH
- sarkoidóza imunologie MeSH
- senioři MeSH
- T-lymfocyty - podskupiny * MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
In this study, we have characterised postnatal changes in T lymphocyte subsets, especially gammadelta T lymphocytes, in blood, spleen and lymph nodes. Detection was carried out using two-colour flow cytometry and three-colour immunohistochemistry. During ontogeny, there was a significant increase in the total percentage of gammadelta T cells in the spleen and blood. In the lymph nodes, there were no age-dependent changes in the total percentage of gammadelta T cells, but the percentage of the gammadeltaTCR+CD8+ subpopulation significantly increased. The tissue distribution of gammadeltaTCR+CD8+ and gammadeltaTCR+CD8- cells in the lymph nodes is random and not collocated with a particular area of the organ. Furthermore, postnatal development was characterised by an increasing frequency of CD8+CD3+CD4-gammadeltaTCR-, which was compensated by a decreasing proportion of CD4+ lymphocytes. Double positive CD4+CD8+ lymphocytes were rare during the first month of life and a significant age-dependent increase of these cells was found in all the compartments monitored.
- MeSH
- imunitní systém růst a vývoj MeSH
- imunohistochemie MeSH
- lymfoidní tkáň cytologie růst a vývoj imunologie MeSH
- novorozená zvířata MeSH
- prasata imunologie MeSH
- průtoková cytometrie MeSH
- receptory antigenů T-buněk gama-delta imunologie MeSH
- T-lymfocyty - podskupiny cytologie imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- receptory antigenů T-buněk gama-delta MeSH
We aimed to detect the levels of T-lympho-cyte subsets and serum tumour markers in patients with non-small cell lung cancer (NSCLC) before chemotherapy, and to investigate the predictive value of their combined detection for the prognosis of NSCLC patients undergoing chemotherapy. The clinical data of 110 NSCLC patients treated with chemotherapy from January 2019 to February 2021 were analysed retrospectively. All patients were followed up for one year and divided into good prognosis group (surviving cases) and poor prognosis group (deceased cases). The predictive value of T-lymphocyte subsets combined with serum tumour markers for prognosis was analysed. The proportions of patients with tumour-node-metastasis stages III-IV, lymph node metastasis and poor differentiation were higher in the poor prognosis group than those in the good prognosis group (P < 0.05). Cox regression analysis revealed that high expression of CD4+ and CEA represented protective factors for poor prognosis of NSCLC patients undergoing chemotherapy [odds ratio (OR) < 1, P < 0.05], while high expression of CA125 was a risk factor (OR > 1, P < 0.05). All the areas under the receiver operating characteristic curves of single indicator detection (CD4+, CEA and CA125 levels) and their combined detection for prediction of the poor prognosis of NSCLC patients undergoing chemotherapy were > 0.70, which was highest in the case of combined detection. T-lymphocyte subsets and serum tumour markers are closely related to the prognosis of NSCLC patients undergoing chemotherapy, and their combined detection is of high predictive value.
- Klíčová slova
- T-lymphocyte subset, non-small cell lung cancer, prediction, prognosis, tumour marker,
- MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádorové biomarkery * krev MeSH
- nádory plic * farmakoterapie krev patologie MeSH
- nemalobuněčný karcinom plic * farmakoterapie krev MeSH
- prediktivní hodnota testů MeSH
- prognóza MeSH
- retrospektivní studie MeSH
- senioři MeSH
- T-lymfocyty - podskupiny * MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- nádorové biomarkery * MeSH
The distribution of T-lymphocyte subsets was studied in patients with myocardial infarction, peripheral arterial disease and thrombophlebitis. The percentage of SRBC-rosette forming active - E[A] and total - E[T] lymphocytes in patients and healthy controls was not different. A significant increase in the percentage and number of theophylline-resistant - E[Thr] and T[M] - helper cells was found in patients with myocardial infarction or with thrombophlebitis, and a moderate elevation was observed in patients with peripheral arterial disease. No difference between patients and controls could be shown in the percentage and number of theophylline-sensitive E[Ths] and T[G] - suppressor cells. The imbalance of T-lymphocyte subsets is thought to be the consequence of sensitization against vascular tissues during vascular diseases.
- MeSH
- arteriální okluzní nemoci imunologie MeSH
- infarkt myokardu imunologie MeSH
- lidé MeSH
- počet leukocytů MeSH
- regulační T-lymfocyty imunologie MeSH
- T-lymfocyty pomocné-indukující imunologie MeSH
- T-lymfocyty imunologie MeSH
- tromboflebitida imunologie MeSH
- tvorba rozet MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Selective deficiency of immunoglobulin A (IgAD) and common variable immunodeficiency (CVID) are genetically closely related diseases, both of unknown pathogenesis. A plethora of abnormalities in lymphocyte subpopulations and expression of activation markers were repeatedly documented in CVID patients, while almost no data are available about lymphocyte subpopulations in IgAD patients. We determined basic lymphocyte subpopulations and those subpopulations that were reported to be abnormal in CVID patients (CD25, human leucocyte antigen (HLA)-DR CD45RA, CD45RO, CD27, CD28 and CD29 on both CD4(+) and CD8(+) cells, CD57 and CD38 on CD8(+) cells, CD21, CD27, IgM, IgD on B lymphocytes) in 85 patients with IgAD, 47 patients with CVID and in 65 healthy controls. Statistical analysis was performed by the Mann-Whitney U-test; significant P-values were determined by means of Bonferoni's correction. Our results showed an increase in the relative number of CD8(+) cells and a decrease in the absolute number of CD4(+) cells compared to healthy people, but similar abnormalities in CVID patients were much more expressed. IgAD patients had significantly decreased expression of HLA-DR and increased expression of CD25 on CD4(+) lymphocytes, also CD29 expression was decreased on CD8(+) cells, while other activation/differentiation markers on T cells (including the expression of CD45RA and CD45RO antigens) were not changed. There were no statistically significant abnormalities in B lymphocyte developmental stages in IgAD patients compared to healthy controls. Our observation showed that the majority of T and B lymphocyte subpopulation abnormalities described previously in CVID are not present in IgAD patients.
- MeSH
- aktivace lymfocytů imunologie MeSH
- běžná variabilní imunodeficience imunologie MeSH
- buněčná diferenciace imunologie MeSH
- CD8-pozitivní T-lymfocyty imunologie MeSH
- deficience IgA imunologie MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- počet CD4 lymfocytů MeSH
- počet lymfocytů MeSH
- podskupiny B-lymfocytů imunologie MeSH
- senioři MeSH
- T-lymfocyty - podskupiny imunologie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Type 1 diabetes mellitus (T1D) and multiple sclerosis (MS) are organ-specific autoimmune diseases leading to an attack of auto-aggressive lymphocytes against the pancreatic beta-cells and central nervous system, respectively. Using four-colour flow cytometry, T-lymphocyte populations having an important function in autoimmune processes were analyzed. T-regulatory cells (Treg) CD4(+)CD25(+)CD127(low), T-suppressor cells (Ts) CD8(+)CD28(-), activated helper CD4(+)CD25(+)CD127(+) and cytotoxic CD8(+)CD25(+) T-cells and also naive CD4(+)CD45RA(+) and memory T-cells CD4(+)CD45RO(+) were compared in the group of patients with T1D (n=30), MS (n=31) and in the group of healthy controls (n=29). Significant differences in Ts cells, activated helper and cytotoxic cells and also memory T-cells were recognized in the group of T1D patients compared to healthy controls. Ts population was significantly lowered in MS patients as well. However, no significant differences were noticed in Treg population. The observed data demonstrate significant differences among patients with T1D and MS in comparison to healthy individuals.
- MeSH
- antigeny CD28 imunologie MeSH
- CD8-pozitivní T-lymfocyty cytologie imunologie MeSH
- diabetes mellitus 1. typu imunologie MeSH
- dospělí MeSH
- imunologická paměť imunologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- počet lymfocytů * MeSH
- průtoková cytometrie metody MeSH
- relabující-remitující roztroušená skleróza imunologie MeSH
- T-lymfocyty - podskupiny cytologie imunologie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny CD28 MeSH
A study was made of changes in the ratio of CD4+/CD8+ in patients who had received cadaverous kidneys and who were under treatment with a combination of cyclosporin A + azathioprine + cor ticosteroids. The results showed that one month after the transparent there was a significant drop (P less than 0.05) in the immunoregulation index compared with the pretransplantation values. Throughout the whole period a correlation (r = -0.65, P less than 0.05) was found between the CD4+/CD8+ ratio and serum, creatinine. In recipients with acute rejection episode no significant difference was found between the CD4+/CD8+ values before the rejection episode (7 +/- 1 days and 3 +/- 1 days) and after it was diagnosed. The introduction of monoclonal antibodies specific for determinants in T-lymphocyte transplantation. Many authors have investigated changes in the CD4+ (helper inducer) to subpopulations (1) made possible immunological monitoring following the organ CDB+ (cytotoxic suppressor) ratio following the organ transplant (2, 3, 4, 5). Some reports (6, 7) have indicated that during rejection of a transplanted kidney there is a rise in the immunoregulation index (CD4+/CD8+), while other works (8, 9) failed to confirm this. Our work presents the dynamics of changes in the CD4+/CD8+ ratio in peripheral blood of recipients of cadaverous kidney transplants, and points to changes in the immunoregulation index before and during acute rejection episodes.
- MeSH
- akutní nemoc MeSH
- antigeny CD4 analýza MeSH
- antigeny CD8 MeSH
- cyklosporiny krev MeSH
- diferenciační antigeny T-lymfocytů analýza MeSH
- dospělí MeSH
- kreatinin krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- rejekce štěpu * MeSH
- T-lymfocyty - podskupiny * MeSH
- transplantace ledvin * MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antigeny CD4 MeSH
- antigeny CD8 MeSH
- cyklosporiny MeSH
- diferenciační antigeny T-lymfocytů MeSH
- kreatinin MeSH
