Providing information on the main approaches for the analysis of metabolites, this textbook: Covers basic methodologies in sample preparation and separation techniques, as well as the most recent techniques of mass spectrometry. Differentiates between primary and secondary metabolites. Includes four chapters discussing successful metabolome studies of different organisms. Highlights the analytical challenges of studying metabolites. Illustrates applications of metabolome analysis through the use of case studies.

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Metabolomics in functional genomics and systems biology -- The chemical challenge of the metabolome -- Sampling and sample preparation -- Analytical tools -- Data analysis -- Yeast metabolomics: the discovery of new metabolic pathways in saccharomyces cerevisiae -- Microbial metabolomics: rapid sampling techniques to investigate intracellular metabolite dynamics--an overview -- Plant metabolomics -- Mass profiling of fungal extracts from pinicillium species -- Metabolomics in humans and other mammals.