• Je něco špatně v tomto záznamu ?

Preparation and testing of stationary phases and modified capillaries for affinity chromatography and affinity capillary electrophoresis of pepsin

Varilová T, Vránková A, Pacáková V, Tichá M, Stulík K

. 2005 ; 1084 (1-2) : 207-213.

Jazyk angličtina Země Nizozemsko

Perzistentní odkaz   https://www.medvik.cz/link/bmc07500895

Three stationary phases have been prepared for affinity liquid chromatography isolation and separation of porcine and human pepsin. The phases contain 3,5-diiodo-L-tyrosine (DIT) bound to the supports HEMA BIO VS, HEMA BIO E and EPOXY TOYOPEARL. These phases have been tested on a model sample of porcine pepsin A and applied to human pepsin. Fractions have been collected and the chymase activity determined in selected analyses. For affinity CE, capillaries have been prepared by modifying the wall with 3-aminopropyltriethoxysilane, followed either by direct binding of DIT, or by binding L-tyrosine that was subsequently iodated. The dissociation constant K(d) has been determined for the pepsin-DIT complex from the changes in the electrophoretic mobilities.

Citace poskytuje Crossref.org

000      
02751naa 2200421 a 4500
001      
bmc07500895
003      
CZ-PrNML
005      
20111210120958.0
008      
070918s2005 ne e eng||
009      
AR
024    __
$a 10.1016/j.chroma.2004.10.022 $2 doi
035    __
$a (PubMed)16114256
040    __
$a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Vařilová, Tereza $7 xx0118800
245    10
$a Preparation and testing of stationary phases and modified capillaries for affinity chromatography and affinity capillary electrophoresis of pepsin / $c Varilová T, Vránková A, Pacáková V, Tichá M, Stulík K
314    __
$a Department of Analytical Chemistry, Charles University, Prague, Hlavova 2030/8, Praha 2, 12843 Czech Republic. tereza.varilova@seznam.cz
520    9_
$a Three stationary phases have been prepared for affinity liquid chromatography isolation and separation of porcine and human pepsin. The phases contain 3,5-diiodo-L-tyrosine (DIT) bound to the supports HEMA BIO VS, HEMA BIO E and EPOXY TOYOPEARL. These phases have been tested on a model sample of porcine pepsin A and applied to human pepsin. Fractions have been collected and the chymase activity determined in selected analyses. For affinity CE, capillaries have been prepared by modifying the wall with 3-aminopropyltriethoxysilane, followed either by direct binding of DIT, or by binding L-tyrosine that was subsequently iodated. The dissociation constant K(d) has been determined for the pepsin-DIT complex from the changes in the electrophoretic mobilities.
650    _2
$a chromatografie afinitní $x metody $x přístrojové vybavení $7 D002846
650    _2
$a elektroforéza kapilární $x metody $x přístrojové vybavení $7 D019075
650    _2
$a pepsin A $x izolace a purifikace $7 D010434
650    _2
$a dijodtyrosin $x chemie $7 D004105
650    _2
$a oxid křemičitý $x chemie $7 D012822
650    _2
$a prasata $7 D013552
650    _2
$a zvířata $7 D000818
650    _2
$a lidé $7 D006801
650    _2
$a finanční podpora výzkumu jako téma $7 D012109
700    1_
$a Pacáková, Věra, $d 1940- $7 jn20000401922
700    1_
$a Tichá, Marie, $d 1938-2017 $7 jn20000710640
700    1_
$a Štulík, Karel, $d 1941-2013 $7 jk01131560
700    1_
$a Vránková, Alice $7 xx0096734
773    0_
$w MED00004968 $t Journal of chromatography. A, Including electrophoresis, mass spectrometry and other separation and detection methods $g Roč. 1084, č. 1-2 (2005), s. 207-213 $x 0021-9673
910    __
$a ABA008 $b x $y 1
990    __
$a 20070918133555 $b ABA008
991    __
$a 20090716130515 $b ABA008
999    __
$a ok $b bmc $g 616505 $s 468937
BAS    __
$a 3
BMC    __
$a 2005 $b 1084 $c 1-2 $d 207-213 $m Journal of chromatography. A, Including electrophoresis and other separation methods $x MED00004962
LZP    __
$a 2007-2/jtjt

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé