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Expression of actin isoforms in human auricular cartilage
R. Kaňa, P. Dundr, D. Tvrdík, E. Nečas, C. Povýšil
Language English Country Czech Republic
Document type Comparative Study
Grant support
NR8150
MZ0
CEP Register
Digital library NLK
Full text - Část
Source
NLK
Free Medical Journals
from 2000
Freely Accessible Science Journals
from 2000
ProQuest Central
from 2005-01-01
Health & Medicine (ProQuest)
from 2005-01-01
ROAD: Directory of Open Access Scholarly Resources
from 2000
- MeSH
- Actins genetics classification MeSH
- Antigens, CD34 genetics MeSH
- Staining and Labeling methods utilization MeSH
- Chondrocytes cytology MeSH
- Financing, Government MeSH
- Humans MeSH
- Reverse Transcriptase Polymerase Chain Reaction methods utilization MeSH
- Protein Isoforms genetics MeSH
- Ear Cartilage cytology growth & development MeSH
- Check Tag
- Humans MeSH
- Publication type
- Comparative Study MeSH
The objective of this study was to determine whether human auricular chondrocytes can also express ?--smooth muscle actin. Immunohistochemistry using monoclonal antibodies for ?-smooth actin, muscle-specific actin, ß-actin, S-100 protein, CD34, and desmin was performed on samples of human ear cartilage obtained from 20 individuals during a partial resection of the ear for different reasons. Moreover, the RT-PCR analysis of actin isoforms in auricular chondrocytes was performed. Approximately 60 % of the chondrocytes of the ear cartilage expressed ?-smooth muscle actin as demonstrated by immunohistochemistry in all the examined samples. Actin-positive chondrocytes occurred in both external subperichondrial layers of the auricular cartilage. This finding was confirmed by the RT-PCR technique. The knowledge of this fact could help us to better understand the chondrocyte changes occurring during the healing and transplantation of auricular cartilage. The question of whether it is necessary to refer to these predominating cells in ear cartilage as myochondrocytes is considered. This is the first report of an unusual immunophenotype and contractile potential for human auricular chondrocytes.
Grant č. NR/8150-4 Ministry of Health CR
Bibliography, etc.Lit.: 13
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- $a Department of Otorhinolaryngology, 1st Faculty of Medicine, Charles University, Prague
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- $a The objective of this study was to determine whether human auricular chondrocytes can also express ?--smooth muscle actin. Immunohistochemistry using monoclonal antibodies for ?-smooth actin, muscle-specific actin, ß-actin, S-100 protein, CD34, and desmin was performed on samples of human ear cartilage obtained from 20 individuals during a partial resection of the ear for different reasons. Moreover, the RT-PCR analysis of actin isoforms in auricular chondrocytes was performed. Approximately 60 % of the chondrocytes of the ear cartilage expressed ?-smooth muscle actin as demonstrated by immunohistochemistry in all the examined samples. Actin-positive chondrocytes occurred in both external subperichondrial layers of the auricular cartilage. This finding was confirmed by the RT-PCR technique. The knowledge of this fact could help us to better understand the chondrocyte changes occurring during the healing and transplantation of auricular cartilage. The question of whether it is necessary to refer to these predominating cells in ear cartilage as myochondrocytes is considered. This is the first report of an unusual immunophenotype and contractile potential for human auricular chondrocytes.
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