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Využití elektrochemických technik pro analýzu biologických vzorků
[Utilizing electrochemical techniques for detection of biological samples]
Ondřej Zítka, Karel Stejskal, Andrea Kleckerová, Vojtěch Adam, Miroslava Beklová, Aleš Horna, Veronika Šupálková, Ladislav Havel, René Kizek
Language Czech Country Czech Republic
- MeSH
- Electrochemistry methods MeSH
- Financing, Government MeSH
- Plants, Edible MeSH
- Cadmium analysis adverse effects MeSH
- Zea mays metabolism MeSH
- Soil Pollutants MeSH
- Sulfhydryl Compounds analysis MeSH
Heavy metals rank among the most toxic compounds occurring in the environment; they are also dangerous due to bioaccumulation. Plants and animals have developed a number of protective mechanisms. The detoxification mechanisms of heavy metals in different organisms have been intensively studied for many years. We aimed at investigation of detoxification mechanisms of maize plants treated with 0, 50, 100, 150, 200, 400 and 500 µM Cd(II) solutions for six days. In particular, we observed their growth and determined the Cd content (by differential pulse anodic stripping voltammetry) and thiol concentration (by HPLC) in the treated plants. Maize plants took up 6 pg of Cd per gram per hour at the lowest dose and 23 pg Cd per g per hour at the highest Cd dose. The relations of glutathione and phytochelatin contents, applied Cd dose, cultivation time, growth curve and plant morphology were investigated.
Utilizing electrochemical techniques for detection of biological samples
Grant č. 522/07/0692 GAČR -- Grant č. 6215712402 MSMT -- Grant č. 1 M06030
Bibliography, etc.Lit.: 32
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- $a Heavy metals rank among the most toxic compounds occurring in the environment; they are also dangerous due to bioaccumulation. Plants and animals have developed a number of protective mechanisms. The detoxification mechanisms of heavy metals in different organisms have been intensively studied for many years. We aimed at investigation of detoxification mechanisms of maize plants treated with 0, 50, 100, 150, 200, 400 and 500 µM Cd(II) solutions for six days. In particular, we observed their growth and determined the Cd content (by differential pulse anodic stripping voltammetry) and thiol concentration (by HPLC) in the treated plants. Maize plants took up 6 pg of Cd per gram per hour at the lowest dose and 23 pg Cd per g per hour at the highest Cd dose. The relations of glutathione and phytochelatin contents, applied Cd dose, cultivation time, growth curve and plant morphology were investigated.
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