• Something wrong with this record ?

Mass spectroscopy analyses of rat 2b myosin heavy chain isoform

J. Žurmanová, D. Maláčová, F. Půta, P. .Novák, J. Říčný, Tomáš Soukup

. 2007 ; 56 (5) : 659-662.

Language English Country Czech Republic

Persistent link   https://www.medvik.cz/link/bmc07508929

We have separated 2b myosin heavy chain (MyHC) isoform from the rat extensor digitorum longus muscle by SDSPAGE and analyzed it by two subsequent mass spectrometry techniques. After tryptic digestion, the obtained peptides were identified by Matrix-Assisted Laser Desorption/Ionisation reflectron Time of Flight mass spectrometry (MALDITOF MS) and sequenced by Liquid chromatography tandem mass spectrometry (ESI LC/MS/MS). The analyzed peptides proportionally covered 30 % of the 2b MyHC isoform sequence. The results suggest that the primary structure is identical with the highest probability to a NCBI database record ref|NP_062198.1|, representing the last updated record of rat 2b isoform. Nonetheless, four peptides carrying amino acid substitution(s) in comparison with the NCBI database record were identified.

Bibliography, etc.

Lit.: 14

000      
02792naa 2200433 a 4500
001      
bmc07508929
003      
CZ-PrNML
005      
20111215102730.0
008      
080918s2007 xr e eng||
009      
AR
040    __
$a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Žurmanová, Jitka. $7 uk2008403153
245    10
$a Mass spectroscopy analyses of rat 2b myosin heavy chain isoform / $c J. Žurmanová, D. Maláčová, F. Půta, P. .Novák, J. Říčný, Tomáš Soukup
314    __
$a Institute of Physiology, Academy of Science of the Czech Republic, Prague
504    __
$a Lit.: 14
520    9_
$a We have separated 2b myosin heavy chain (MyHC) isoform from the rat extensor digitorum longus muscle by SDSPAGE and analyzed it by two subsequent mass spectrometry techniques. After tryptic digestion, the obtained peptides were identified by Matrix-Assisted Laser Desorption/Ionisation reflectron Time of Flight mass spectrometry (MALDITOF MS) and sequenced by Liquid chromatography tandem mass spectrometry (ESI LC/MS/MS). The analyzed peptides proportionally covered 30 % of the 2b MyHC isoform sequence. The results suggest that the primary structure is identical with the highest probability to a NCBI database record ref|NP_062198.1|, representing the last updated record of rat 2b isoform. Nonetheless, four peptides carrying amino acid substitution(s) in comparison with the NCBI database record were identified.
650    _2
$a těžké řetězce myosinu $x fyziologie $x izolace a purifikace $7 D018995
650    _2
$a spektrometrie hmotnostní - ionizace laserem za účasti matrice $x metody $x využití $7 D019032
650    _2
$a hmotnostní spektrometrie s elektrosprejovou ionizací $x metody $x využití $7 D021241
650    _2
$a potkani inbrední LEW $x fyziologie $7 D011917
650    _2
$a interpretace statistických dat $7 D003627
650    _2
$a polymerázová řetězová reakce s reverzní transkripcí $x metody $x využití $7 D020133
650    _2
$a financování organizované $x využití $7 D005381
700    1_
$a Horníková, Daniela $7 xx0157744
700    1_
$a Půta, František $7 xx0093597
700    1_
$a Novák, Petr. $7 _AN030014
700    1_
$a Říčný, Jan $7 xx0153151
700    1_
$a Soukup, Tomáš $7 xx0140789
773    0_
$w MED00003824 $t Physiological research $g Roč. 56, č. 5 (2007), s. 659-662 $x 0862-8408
856    41
$u http://www.biomed.cas.cz/physiolres/pdf/56/56_659.pdf $y plný text volně přístupný
910    __
$a ABA008 $b A 4120 $c 266 $y 1
990    __
$a 20080916111515 $b ABA008
991    __
$a 20111215102721 $b ABA008
999    __
$a ok $b bmc $g 624523 $s 476958
BAS    __
$a 3
BMC    __
$a 2007 $b 56 $c 5 $d 659-662 $i 0862-8408 $m Physiological research $x MED00003824
LZP    __
$a 2008-22/ipal

Find record

Citation metrics

Archiving options