The role of Chlamydia, Mycoplasma and Ureaplasma species in male fertility has been studied for a long time. But, the information about their negative influence on semen quality and male fertility is still not very clear and the findings differ between authors. The aim of this study was to find if the presence of these microorganisms in male ejaculate can affect sperm quality. Material and methods: Sperm analysis was performed on 742 ejaculates from 627 men consisting of semen volume, sperm concentration, sperm motility, sperm velocity by Baker´s propulsitivity test, and morphology analysis using optical microscopy. The classification of normal spermatozoa was in accordance with the definition of the WHO laboratory manual (1999). Chlamydia in the ejaculates were detected by a direct immunofluorescent reaction using the Progen Biotechnik GmbH diagnostic set for detection of C. trachomatis, C. psittaci, and C. pneumoniae. The Mycoplasma and Ureaplasma detection were done by semen cultivation on PPLO Broth Base and PPLO Agar Base media (HiMedia Laboratories Pvt. Ltd., India). Results: In total 49.2% of samples were contaminated. Chlamydia species alone were found in 13.4%, Mycoplasma species alone in 4.8%, Ureaplasma species alone in 20.6%, and mixed contamination were detected in 10.4% of ejaculates. In the Chlamydia positive group the quality of ejaculates was lower in all evaluated parameters. In the Mycoplasma positive group the sperm concentration was 29.6% lower (p<0.01) but we did not find any statistically significant differences between Ureaplasma positive and the control group in any of the sperm characteristics. Conclusions: The results showed that both Chlamydial and Mycoplasmal contamination affect sperm quality, but we did not find any negative effect of Ureaplasmal contamination on sperm quality. The lower sperm concentration in Mycoplasma positive samples shows that there can be a possible negative influence of the microorganism on testis parenchyma.

Veterinary Research Institute Department of Genetics and Reproduction Brno

3. český a mezinárodní andrologický kongres, Štiřín, 13.-15.6.2008

000      

00000naa 2200000 a 4500

001      

bmc07518206

003      

CZ-PrNML

005      

20120713150554.0

008      

090223s2008 xr e eng||

009      

PC

040    __

$a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xr

100    1_

$a Věžník, Zdeněk, $d 1928-2012 $7 jn20000402652

245    10

$a Do chlamydia, mycoplasma and ureaplasma contaminations affect sperm quality? / $c Veznik, Z., Zajicova, A., Svecova, D., Kunetkova, M., Prinosilova, P.

314    __

$a Veterinary Research Institute, Department of Genetics and Reproduction, Brno

500    __

$a 3. český a mezinárodní andrologický kongres, Štiřín, 13.-15.6.2008

520    9_

$a The role of Chlamydia, Mycoplasma and Ureaplasma species in male fertility has been studied for a long time. But, the information about their negative influence on semen quality and male fertility is still not very clear and the findings differ between authors. The aim of this study was to find if the presence of these microorganisms in male ejaculate can affect sperm quality. Material and methods: Sperm analysis was performed on 742 ejaculates from 627 men consisting of semen volume, sperm concentration, sperm motility, sperm velocity by Baker´s propulsitivity test, and morphology analysis using optical microscopy. The classification of normal spermatozoa was in accordance with the definition of the WHO laboratory manual (1999). Chlamydia in the ejaculates were detected by a direct immunofluorescent reaction using the Progen Biotechnik GmbH diagnostic set for detection of C. trachomatis, C. psittaci, and C. pneumoniae. The Mycoplasma and Ureaplasma detection were done by semen cultivation on PPLO Broth Base and PPLO Agar Base media (HiMedia Laboratories Pvt. Ltd., India). Results: In total 49.2% of samples were contaminated. Chlamydia species alone were found in 13.4%, Mycoplasma species alone in 4.8%, Ureaplasma species alone in 20.6%, and mixed contamination were detected in 10.4% of ejaculates. In the Chlamydia positive group the quality of ejaculates was lower in all evaluated parameters. In the Mycoplasma positive group the sperm concentration was 29.6% lower (p<0.01) but we did not find any statistically significant differences between Ureaplasma positive and the control group in any of the sperm characteristics. Conclusions: The results showed that both Chlamydial and Mycoplasmal contamination affect sperm quality, but we did not find any negative effect of Ureaplasmal contamination on sperm quality. The lower sperm concentration in Mycoplasma positive samples shows that there can be a possible negative influence of the microorganism on testis parenchyma.

590    __

$a NEINDEXOVÁNO

655    _2

$a abstrakty $7 D020504

700    1_

$a Švecová, Drahomíra $7 uzp2011667908

700    1_

$a Kunetková, Monika. $7 _BN006694

700    1_

$a Přinosilová, Petra. $7 _BN006695

700    1_

$a Zajícová, Atanáska. $7 _BN006693

773    0_

$w MED00194845 $t Abstracts of the 3rd Czech and International Congress of Andrology $g Roč. 2,Suppl.1 (2008), s. 37 $x 1802-4793

910    __

$a ABA008 $b B 2486 $y 9

990    __

$a 20090218133734 $b ABA008

991    __

$a 20120713150508 $b ABA008

999    __

$a ok $b bmc $g 636018 $s 488747

BAS    __

$a 6

BMC    __

$a 2008 $b 2 $c Suppl.1 $d 37 $i 1802-4793 $m Abstracts of the 3rd Czech and International Congress of Andrology $x MED00194845

LZP    __

$a 2009-08/mkbi