BACKGROUND: Nestin is a protein belonging to class VI of intermediate filaments that is produced in stem/progenitor cells in the mammalian CNS during development and is consecutively replaced by other intermediate filament proteins (neurofilaments, GFAP). Down-regulated nestin may be re-expressed in the adult organism under certain pathological conditions (brain injury, ischemia, inflammation, neoplastic transformation). Our work focused on a detailed study of the nestin cytoskeleton in cell lines derived from glioblastoma multiforme, because re-expression of nestin together with down-regulation of GFAP has been previously reported in this type of brain tumor. METHODS: Two cell lines were derived from the tumor tissue of patients treated for glioblastoma multiforme. Nestin and other cytoskeletal proteins were visualized using imunocytochemical methods: indirect immunofluorescence and immunogold-labelling. RESULTS: Using epifluorescence and confocal microscopy, we described the morphology of nestin-positive intermediate filaments in glioblastoma cells of both primary cultures and the derived cell lines, as well as the reorganization of nestin during mitosis. Our most important result came through transmission electron microscopy and provided clear evidence that nestin is present in the cell nucleus. CONCLUSION: Detailed information concerning the pattern of the nestin cytoskeleton in glioblastoma cell lines and especially the demonstration of nestin in the nucleus represent an important background for further studies of nestin re-expression in relationship to tumor malignancy and invasive potential.

Department of Biology School of Medicine Masaryk University Brno Czech Republic

000      

00000naa 2200000 a 4500

001      

bmc07520021

003      

CZ-PrNML

005      

20111210130957.0

008      

090326s2006 xxk e eng||

009      

AR

040    __

$a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xxk

100    1_

$a Veselská, Renata, $d 1968- $7 mzk2003196540

245    10

$a Nestin expression in the cell lines derived from glioblastoma multiforme / $c Veselska R, Kuglik P, Cejpek P, Svachova H, Neradil J, Loja T, Relichova J.

314    __

$a Department of Biology, School of Medicine, Masaryk University, Brno, Czech Republic. rvesel@med.muni.cz

520    9_

$a BACKGROUND: Nestin is a protein belonging to class VI of intermediate filaments that is produced in stem/progenitor cells in the mammalian CNS during development and is consecutively replaced by other intermediate filament proteins (neurofilaments, GFAP). Down-regulated nestin may be re-expressed in the adult organism under certain pathological conditions (brain injury, ischemia, inflammation, neoplastic transformation). Our work focused on a detailed study of the nestin cytoskeleton in cell lines derived from glioblastoma multiforme, because re-expression of nestin together with down-regulation of GFAP has been previously reported in this type of brain tumor. METHODS: Two cell lines were derived from the tumor tissue of patients treated for glioblastoma multiforme. Nestin and other cytoskeletal proteins were visualized using imunocytochemical methods: indirect immunofluorescence and immunogold-labelling. RESULTS: Using epifluorescence and confocal microscopy, we described the morphology of nestin-positive intermediate filaments in glioblastoma cells of both primary cultures and the derived cell lines, as well as the reorganization of nestin during mitosis. Our most important result came through transmission electron microscopy and provided clear evidence that nestin is present in the cell nucleus. CONCLUSION: Detailed information concerning the pattern of the nestin cytoskeleton in glioblastoma cell lines and especially the demonstration of nestin in the nucleus represent an important background for further studies of nestin re-expression in relationship to tumor malignancy and invasive potential.

650    _2

$a nádorové buněčné linie $7 D045744

650    _2

$a cytoskelet $x chemie $x ultrastruktura $7 D003599

650    _2

$a fluorescenční protilátková technika nepřímá $7 D019084

650    _2

$a gliový fibrilární kyselý protein $x analýza $7 D005904

650    _2

$a glioblastom $x genetika $x chemie $x patologie $x ultrastruktura $7 D005909

650    _2

$a proteiny intermediálních filament $x analýza $7 D007381

650    _2

$a lidé $7 D006801

650    _2

$a proteiny nervové tkáně $x analýza $7 D009419

650    _2

$a vimentin $x analýza $7 D014746

650    _2

$a financování organizované $7 D005381

700    1_

$a Kuglík, Petr, $d 1957- $7 ola2003204793

700    1_

$a Cejpek, Pavel $7 xx0073420

700    1_

$a Šváchová, Hana $7 xx0112988

700    1_

$a Neradil, Jakub $7 xx0079539

700    1_

$a Loja, Tomáš $7 xx0112989

700    1_

$a Relichová, Jiřina, $d 1945- $7 nlk19990073774

773    0_

$w MED00008171 $t BMC cancer $g Roč. 6 (2006), s. 32 $x 1471-2407

910    __

$a ABA008 $b x $y 9

990    __

$a 20090325104238 $b ABA008

991    __

$a 20090630141554 $b ABA008

999    __

$a ok $b bmc $g 637825 $s 490621

BAS    __

$a 3

BMC    __

$a 2006 $b 6 $d 32 $i 1471-2407 $m BMC cancer $x MED00008171

LZP    __

$a 2009-B1/dkme