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Leptin affects proliferation-, apoptosis- and protein kinase A-related peptides in human ovarian granulosa cells
Alexander V. Sirotkin, Miloš Mlynček, Alexander V. Makarevich, I. Florkovičová, Ladislav Hetényi
Language English Country Czech Republic
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- MeSH
- Apoptosis MeSH
- Cell Cycle MeSH
- Cyclin B metabolism MeSH
- Adult MeSH
- Financing, Organized MeSH
- Granulosa Cells enzymology immunology pathology MeSH
- Immunohistochemistry MeSH
- Cells, Cultured MeSH
- Leptin metabolism MeSH
- Humans MeSH
- Cell Proliferation MeSH
- Proliferating Cell Nuclear Antigen metabolism MeSH
- bcl-2-Associated X Protein metabolism MeSH
- Cyclic AMP-Dependent Protein Kinases metabolism MeSH
- Recombinant Proteins metabolism MeSH
- Signal Transduction MeSH
- Blotting, Western MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Female MeSH
The aim of our in vitro studies was to understand the role of leptin in controlling proliferation, apoptosis, and protein kinase A (PKA) in human ovarian cells. We analyzed the in vitro effects of leptin (0, 1, 10 or 100 ng/ml) on the accumulation of proliferation-related peptides (PCNA, cyclin B1), apoptosis-associated peptide (Bax) and the intracellular signaling molecule PKA in cultured human granulosa cells using immunocytochemistry and Western immunoblotting. It was observed that leptin stimulated in a dose-dependent manner the accumulation of PCNA (at doses 1-100 ng/ml), cyclin B1 (at doses 10 or 100 ng/ml), Bax (at doses 10 or 100 ng/ml) and PKA (at doses 1-100 ng/ml) in cultured human ovarian cells. These observations suggest the ability of leptin to control directly human ovarian cell functions: proliferation, apoptosis, and intracellular messenger PKA.
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Lit.: 19
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- $a Lit.: 19
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- $a The aim of our in vitro studies was to understand the role of leptin in controlling proliferation, apoptosis, and protein kinase A (PKA) in human ovarian cells. We analyzed the in vitro effects of leptin (0, 1, 10 or 100 ng/ml) on the accumulation of proliferation-related peptides (PCNA, cyclin B1), apoptosis-associated peptide (Bax) and the intracellular signaling molecule PKA in cultured human granulosa cells using immunocytochemistry and Western immunoblotting. It was observed that leptin stimulated in a dose-dependent manner the accumulation of PCNA (at doses 1-100 ng/ml), cyclin B1 (at doses 10 or 100 ng/ml), Bax (at doses 10 or 100 ng/ml) and PKA (at doses 1-100 ng/ml) in cultured human ovarian cells. These observations suggest the ability of leptin to control directly human ovarian cell functions: proliferation, apoptosis, and intracellular messenger PKA.
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