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Effect of culture substrate and culture conditions on lens epithelial cell proliferation and ?-smooth muscle actin expression
G. Mahelková, L. Bačáková, J. Korynta, L. Vajner, R. Vytásek
Jazyk angličtina Země Česko
NLK
Free Medical Journals
od 2000
Freely Accessible Science Journals
od 2000
ProQuest Central
od 2005-01-01
Health & Medicine (ProQuest)
od 2005-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2000
- MeSH
- aktiny metabolismus MeSH
- alfa-krystaliny metabolismus MeSH
- beta-krystaliny metabolismus MeSH
- epitelové buňky cytologie metabolismus účinky léků MeSH
- financování organizované MeSH
- gama-krystaliny metabolismus MeSH
- imunohistochemie MeSH
- kolagen farmakologie MeSH
- králíci MeSH
- kultivační média farmakologie MeSH
- kultivované buňky MeSH
- myocyty hladké svaloviny účinky léků MeSH
- oční čočka cytologie MeSH
- prasata MeSH
- proliferace buněk účinky léků MeSH
- regulace genové exprese účinky léků MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
The most common complication following cataract surgery is posterior capsule opacification. This results from migration, proliferation and transdifferentiation of residual lens epithelial cells (LECs). We studied the effect of several culture substrates and culture conditions on LEC proliferation and ?-smooth muscle actin (?-SMA) expression. We used primary and secondary cultures of porcine LECs cultivated on collagen I, collagen IV, microscopic glass slides, and uncoated plastic dishes. We studied the cell proliferation and expression of ?-SMA and ?-, ß-, and ?-crystallins. The effect of the medium exchange protocol was studied using the TOTL-86 rabbit epithelial lens cell line. There was no difference in growth characteristics of primary cultures on different substrates. In secondary cultures, LECs adhered better to collagen-coated surfaces. The culture substrate influenced LEC proliferation and ?-SMA expression. The proliferation was greater when the medium was changed than when extra medium was added on the 4th day. The cells did not synthesize ?-, ß- or ?-crystallin. The culture substrate influences the adhesion ability, proliferation and ?-SMA expression in lens epithelial cells. In addition, it is necessary to consider the effects of the medium exchange protocol, serum supplementation, cell density and other cell culture conditions in lens epithelial cell experiments.
Lit.: 41
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- $a The most common complication following cataract surgery is posterior capsule opacification. This results from migration, proliferation and transdifferentiation of residual lens epithelial cells (LECs). We studied the effect of several culture substrates and culture conditions on LEC proliferation and ?-smooth muscle actin (?-SMA) expression. We used primary and secondary cultures of porcine LECs cultivated on collagen I, collagen IV, microscopic glass slides, and uncoated plastic dishes. We studied the cell proliferation and expression of ?-SMA and ?-, ß-, and ?-crystallins. The effect of the medium exchange protocol was studied using the TOTL-86 rabbit epithelial lens cell line. There was no difference in growth characteristics of primary cultures on different substrates. In secondary cultures, LECs adhered better to collagen-coated surfaces. The culture substrate influenced LEC proliferation and ?-SMA expression. The proliferation was greater when the medium was changed than when extra medium was added on the 4th day. The cells did not synthesize ?-, ß- or ?-crystallin. The culture substrate influences the adhesion ability, proliferation and ?-SMA expression in lens epithelial cells. In addition, it is necessary to consider the effects of the medium exchange protocol, serum supplementation, cell density and other cell culture conditions in lens epithelial cell experiments.
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