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Isolation of Lawsonia intracellularis specific single-chain Fv antibody fragments from phage display library
Dezorzová-Tomanová K, Molinková D, Pekarová M, Celer V, Smola J.
Language English Country Great Britain
NLK
ScienceDirect (archiv)
from 1993-01-01 to 2009-12-31
- MeSH
- Financing, Organized MeSH
- Lawsonia Bacteria immunology MeSH
- Humans MeSH
- Peptide Library MeSH
- Antibodies, Bacterial genetics immunology isolation & purification MeSH
- Antibody Specificity MeSH
- Immunoglobulin Variable Region genetics immunology isolation & purification MeSH
- Check Tag
- Humans MeSH
Single-chain antibodies (scFv) exhibiting specific binding to Lawsonia intracellularis were isolated from a phagemid library expressing scFvs molecules on the surface of filamentous bacteriophages. For scFv selection whole bacterial cells were used and individual clones were tested in ELISA test. The total of seven unique clones with different fingerprint profiles was isolated. All clones were able to bind specifically in immunofluorescence assay. This is the first report of species specific recombinant antibodies against L. intracellularis.
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- $a Single-chain antibodies (scFv) exhibiting specific binding to Lawsonia intracellularis were isolated from a phagemid library expressing scFvs molecules on the surface of filamentous bacteriophages. For scFv selection whole bacterial cells were used and individual clones were tested in ELISA test. The total of seven unique clones with different fingerprint profiles was isolated. All clones were able to bind specifically in immunofluorescence assay. This is the first report of species specific recombinant antibodies against L. intracellularis.
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