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Isolation of Lawsonia intracellularis specific single-chain Fv antibody fragments from phage display library
Dezorzová-Tomanová K, Molinková D, Pekarová M, Celer V, Smola J.
Jazyk angličtina Země Velká Británie
NLK
ScienceDirect (archiv)
od 1993-01-01 do 2009-12-31
- MeSH
- financování organizované MeSH
- Lawsonia (bakterie) imunologie MeSH
- lidé MeSH
- peptidová knihovna MeSH
- protilátky bakteriální genetika imunologie izolace a purifikace MeSH
- specificita protilátek MeSH
- variabilní oblast imunoglobulinu genetika imunologie izolace a purifikace MeSH
- Check Tag
- lidé MeSH
Single-chain antibodies (scFv) exhibiting specific binding to Lawsonia intracellularis were isolated from a phagemid library expressing scFvs molecules on the surface of filamentous bacteriophages. For scFv selection whole bacterial cells were used and individual clones were tested in ELISA test. The total of seven unique clones with different fingerprint profiles was isolated. All clones were able to bind specifically in immunofluorescence assay. This is the first report of species specific recombinant antibodies against L. intracellularis.
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- $a Single-chain antibodies (scFv) exhibiting specific binding to Lawsonia intracellularis were isolated from a phagemid library expressing scFvs molecules on the surface of filamentous bacteriophages. For scFv selection whole bacterial cells were used and individual clones were tested in ELISA test. The total of seven unique clones with different fingerprint profiles was isolated. All clones were able to bind specifically in immunofluorescence assay. This is the first report of species specific recombinant antibodies against L. intracellularis.
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