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Basidiomycete cultures on perlite survive successfully repeated freezing and thawing in cryovials without subculturing
Homolka L, Lisá L, Nerud F.
Journal of microbiological methods.
2007 ;
69 (3) :
529-532.
Language English Country Netherlands
Document type Evaluation Study
Persistent link
https://www.medvik.cz/link/bmc10001436
- MeSH
- Basidiomycota classification growth & development MeSH
- Financing, Organized MeSH
- Cryopreservation methods instrumentation MeSH
- Culture Media MeSH
- Mycology methods MeSH
- Aluminum Oxide MeSH
- Freezing MeSH
- Publication type
- Evaluation Study MeSH
Mycelial basidiomycete cultures on perlite in cryovials survived successfully three successive cycles of freezing, storage in liquid nitrogen (LN) and thawing without noticeable changes. This indicates that using perlite as a carrier for cryopreservation could in most cases overcome difficulties caused by interrupted supply of LN or electric power during the storage. Cultures on perlite can also be reused for successive inoculations.
References provided by Crossref.org
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