AIMS: Proinflammatory cytokines are thought to play an important role in various kidney graft diseases resulting in interstitial fibrosis and tubular atrophy frequently found in case biopsies. To explore the role of various cytokines and chemokines in the long-term graft outcome, the transcription patterns of their genes in kidney allograft biopsies were evaluated. METHODS: The real-time RT-PCR was used to identify intragraft mRNA expression of cytokines and chemokines in 74 kidney graft recipients and the results were correlated with histological and clinical parameters and long-term graft outcome. RESULTS: We observed up-regulated IL-10 (p < 0.001), TGF-beta1, IL-6, MCP-1, RANTES (p < 0.01) and TNF-alpha (p < 0.05) mRNA expression in patients with chronic allograft nephropathy (CAN) as compared to controls. There were positive correlations between the mRNA expression of IL-6 (p < 0.001), IL-10 (p < 0.01), TNF-alpha, MCP-1 (p < 0.05) and the proteinuria. The up-regulation of intrarenal MCP-1 in patients with CAN increased the risk for the graft failure within the next 42 months (OR 5.1, p < 0.05). Kaplan-Meier survival analysis revealed that proteinuria and higher intragraft expression of TGF-beta1 and MCP-1 predict a poor kidney graft outcome. CONCLUSION: Expression patterns of intrarenal proinflammatory genes might discriminate patients at a higher risk for the earlier allograft failure. 2007 S. Karger AG, Basel

Transplant Laboratory Institute for Clinical and Experimental Medicine Prague Czech Republic

000      

03205naa 2200505 a 4500

001      

bmc10012811

003      

CZ-PrNML

005      

20130627204614.0

008      

100527s2007 sz e eng||

009      

AR

040    __

$a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a sz

100    1_

$a Hřibová, Petra $7 xx0143051

245    10

$a Intrarenal cytokine and chemokine gene expression and kidney graft outcome / $c P. Hribova, J. Lacha, K. Kotsch, H.D. Volk, I. Brabcova, J. Skibova, S. Vitko, O. Viklicky

314    __

$a Transplant Laboratory, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.

520    9_

$a AIMS: Proinflammatory cytokines are thought to play an important role in various kidney graft diseases resulting in interstitial fibrosis and tubular atrophy frequently found in case biopsies. To explore the role of various cytokines and chemokines in the long-term graft outcome, the transcription patterns of their genes in kidney allograft biopsies were evaluated. METHODS: The real-time RT-PCR was used to identify intragraft mRNA expression of cytokines and chemokines in 74 kidney graft recipients and the results were correlated with histological and clinical parameters and long-term graft outcome. RESULTS: We observed up-regulated IL-10 (p < 0.001), TGF-beta1, IL-6, MCP-1, RANTES (p < 0.01) and TNF-alpha (p < 0.05) mRNA expression in patients with chronic allograft nephropathy (CAN) as compared to controls. There were positive correlations between the mRNA expression of IL-6 (p < 0.001), IL-10 (p < 0.01), TNF-alpha, MCP-1 (p < 0.05) and the proteinuria. The up-regulation of intrarenal MCP-1 in patients with CAN increased the risk for the graft failure within the next 42 months (OR 5.1, p < 0.05). Kaplan-Meier survival analysis revealed that proteinuria and higher intragraft expression of TGF-beta1 and MCP-1 predict a poor kidney graft outcome. CONCLUSION: Expression patterns of intrarenal proinflammatory genes might discriminate patients at a higher risk for the earlier allograft failure. 2007 S. Karger AG, Basel

650    _2

$a dospělí $7 D000328

650    _2

$a chemokiny $x biosyntéza $x genetika $7 D018925

650    _2

$a cytokiny $x biosyntéza $x genetika $7 D016207

650    _2

$a ženské pohlaví $7 D005260

650    _2

$a následné studie $7 D005500

650    _2

$a regulace genové exprese $x fyziologie $7 D005786

650    _2

$a rejekce štěpu $x genetika $x metabolismus $7 D006084

650    _2

$a přežívání štěpu $x genetika $7 D006085

650    _2

$a lidé $7 D006801

650    _2

$a ledviny $x metabolismus $x patologie $7 D007668

650    _2

$a transplantace ledvin $7 D016030

650    _2

$a mužské pohlaví $7 D008297

650    _2

$a lidé středního věku $7 D008875

650    _2

$a výsledek terapie $7 D016896

650    _2

$a financování organizované $7 D005381

655    _2

$a srovnávací studie $7 D003160

700    1_

$a Lácha, Jiří, $d 1960-2005 $7 nlk20010095504

700    1_

$a Kotsch, Katja

700    1_

$a Volk, Hans-Dieter

700    1_

$a Brabcová, Irena $7 xx0079327

700    1_

$a Skibová, Jelena $7 xx0061183

700    1_

$a Vítko, Štefan, $d 1953- $7 jn20000402658

700    1_

$a Viklický, Ondřej, $d 1966- $7 nlk20050170291

773    0_

$t Kidney & Blood Pressure Research $w MED00003064 $g Roč. 30, č. 5 (2007), s. 273-282

910    __

$a ABA008 $b x $y 8

990    __

$a 20100602083136 $b ABA008

991    __

$a 20130627205106 $b ABA008

999    __

$a ok $b bmc $g 726666 $s 589823

BAS    __

$a 3

BMC    __

$a 2007 $b 30 $c 5 $d 273-282 $m Kidney & blood pressure research $n Kidney Blood Press Res $x MED00003064

LZP    __

$a 2010-B2/vtme