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Improved protocol for high-quality co-extraction of DNA and RNA from rumen digesta

M. Popova, C. Martin, D.P. Morgavi

. 2010 ; 55 (4) : 368-372.

Language English Country Czech Republic

Persistent link   https://www.medvik.cz/link/bmc10025490

We report an improved method for total nucleic acids extraction from rumen content samples. The method employs bead beating, and phenol-chloroform extraction followed by saline-alcohol precipitation. Total nucleic acids and RNA yield and purity were assessed by spectrophotometric measurements; RNA integrity was estimated using Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer. The method provided total nucleic acids and RNA extracts of good quantity and quality. The extraction is not time consuming and it is valuable for ecological studies of rumen microbial community structure and gene expression.

6th International Symposium of Anaerobic Microbiology (ISAM 2009) held in Liblice (Czech Republic), 18 - 20 June 2009

Bibliography, etc.

Lit.: 13

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