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Porovnání chromatografických metod pro stanovení ergosterolu a jejich využití při analýze půdy podhorské pastviny
[Comparison of chromatographic methodsfor ergosterol determination and their application in analysis of upland pasture soil]
Jiří Jirout, Jan Tříska, Kamila Růžičková, Naděžda Vrchotová, Miloslav Šimek, Dana Elhottová
Language Czech Country Czech Republic
Ergosterol, a biomarker of living saprotrophic fungi, was obtained from soil by microwave-assisted extraction and determined by GC-MS-MS, HPLC, and GC-FID. The GC-MS-MS technique was used to monitor fungal biomass in the soils differently impacted by overwintering cattle. The amount of ergosterol in soil increased from 0.93 ± 0.34 to 29.28 ± 3.07 ?g g?1 of soil. The detection limits in GC-MS-MS and HPLC methods were ca. ten times lower than in GC-FID. Whereas GC-MS-MS is the best method for monitoring the complex sterol profile in the environment, the selective MS-MS mode allowed the determination of ergosterol in complex matrices by elimination of coeluting peaks. The enrichment of cattleimpacted soils in non-fungal sterols (campesterol, stigmaserol and ß-sitosterol) and stanols (ergostanol, sitostanol) was due to digested plant materials and rumen microflora, suggesting significant biochemical changes in the pasture soils under study.
Comparison of chromatographic methodsfor ergosterol determination and their application in analysis of upland pasture soil
Lit.: 32
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- $a Comparison of chromatographic methodsfor ergosterol determination and their application in analysis of upland pasture soil
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- $a Ergosterol, a biomarker of living saprotrophic fungi, was obtained from soil by microwave-assisted extraction and determined by GC-MS-MS, HPLC, and GC-FID. The GC-MS-MS technique was used to monitor fungal biomass in the soils differently impacted by overwintering cattle. The amount of ergosterol in soil increased from 0.93 ± 0.34 to 29.28 ± 3.07 ?g g?1 of soil. The detection limits in GC-MS-MS and HPLC methods were ca. ten times lower than in GC-FID. Whereas GC-MS-MS is the best method for monitoring the complex sterol profile in the environment, the selective MS-MS mode allowed the determination of ergosterol in complex matrices by elimination of coeluting peaks. The enrichment of cattleimpacted soils in non-fungal sterols (campesterol, stigmaserol and ß-sitosterol) and stanols (ergostanol, sitostanol) was due to digested plant materials and rumen microflora, suggesting significant biochemical changes in the pasture soils under study.
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