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Identification of CD133+/nestin+ putative cancer stem cells in non-small cell lung cancer

Maria Janikova, Jozef Skarda, Marta Dziechciarkova, Lenka Radova, Jana Chmelova, Veronika Krejci, Eva Sedlakova, Jana Zapletalova, Katerina Langova, Jiri Klein, Ivona Grygarkova, Vitezslav Kolek

. 2010 ; 154 (4) : 321-326.

Language English Country Czech Republic

Persistent link   https://www.medvik.cz/link/bmc11002140

Grant support
NS10285 MZ0 CEP Register

Aims. No effective treatment for lung cancer exists currently. One reason for this, is the development of drug resistance,-assumed to be associated with cancer stem cell (CSCs) emergence within the tumour. This pilot study aimed-to identify CSCs in 121 non-small cell lung cancer (NSCLC) patient samples via detection of the expression of stem-cell markers – CD133 and nestin. Material and methods. Archived paraffin blocks of 121 patient samples were prepared as Tissue Microarrays-(TMA). Indirect immunohistochemical staining was used to determine the level of expression of CD133 and nestin.-Double immunofluorescence staining was used to investigate the co-expression of these two markers. To determine-the correlation between expression of nestin and CD133 with the length of asymptomatic period and overall patient-survival we used the Kaplan-Meyer analysis. Results. CD133 expression was detected in 22 (19%), nestin in the epithelium in 74 (66%) and vasculature in-78 (70%) of patients. Co-expression of these two markers was found in 21 (17%) patients in less than 1% of positive-cells without impact on disease free or overall survival. Conclusions. We identified CD133+/nestin+ cells as novel potential markers of lung cancer CSCs.

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Lit.: 34

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$a Janíková, Mária. $7 xx0267892 $u Department of Pathology and Laboratory of Molecular Pathology, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Czech Republic; Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University and University Hospital
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$a Identification of CD133+/nestin+ putative cancer stem cells in non-small cell lung cancer / $c Maria Janikova, Jozef Skarda, Marta Dziechciarkova, Lenka Radova, Jana Chmelova, Veronika Krejci, Eva Sedlakova, Jana Zapletalova, Katerina Langova, Jiri Klein, Ivona Grygarkova, Vitezslav Kolek
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$a Aims. No effective treatment for lung cancer exists currently. One reason for this, is the development of drug resistance,-assumed to be associated with cancer stem cell (CSCs) emergence within the tumour. This pilot study aimed-to identify CSCs in 121 non-small cell lung cancer (NSCLC) patient samples via detection of the expression of stem-cell markers – CD133 and nestin. Material and methods. Archived paraffin blocks of 121 patient samples were prepared as Tissue Microarrays-(TMA). Indirect immunohistochemical staining was used to determine the level of expression of CD133 and nestin.-Double immunofluorescence staining was used to investigate the co-expression of these two markers. To determine-the correlation between expression of nestin and CD133 with the length of asymptomatic period and overall patient-survival we used the Kaplan-Meyer analysis. Results. CD133 expression was detected in 22 (19%), nestin in the epithelium in 74 (66%) and vasculature in-78 (70%) of patients. Co-expression of these two markers was found in 21 (17%) patients in less than 1% of positive-cells without impact on disease free or overall survival. Conclusions. We identified CD133+/nestin+ cells as novel potential markers of lung cancer CSCs.
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$a Škarda, Jozef $7 xx0098446 $u Department of Pathology and Laboratory of Molecular Pathology, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Czech Republic; Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University and University Hospital
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$a Dziechciarková, Marta $7 xx0080459 $u Department of Pediatrics, Laboratory of Experimental Medicine, Faculty of Medicine and Dentistry, Palacky University and University Hospital
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$a Zapletalová, Jana $7 xx0111614 $u Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University
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$a Langová, Kateřina $7 xx0141371 $u Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University
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$a Klein, Jiří, $d 1963- $7 xx0047429 $u 1st Department of Surgery, Faculty of Medicine and Dentistry, Palacky University and University Hospital
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