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Differentiation of CD133-positive pancreatic cells into insulin-producing islet-like cell clusters
T Koblas, L Pektorova, K Zacharovova, Z Berkova, P Girman, E Dovolilova, L Karasova, F Saudek
Jazyk angličtina Země Spojené státy americké
Grantová podpora
NR9060
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Část
Zdroj
NLK
ScienceDirect (archiv)
od 1997-01-01 do 2009-12-31
- MeSH
- buněčná diferenciace MeSH
- buněčné kultury MeSH
- C-peptid analýza MeSH
- CD antigeny analýza MeSH
- financování organizované MeSH
- glykoproteiny analýza MeSH
- Langerhansovy ostrůvky cytologie fyziologie MeSH
- lidé MeSH
- magnetismus MeSH
- pankreas cytologie MeSH
- peptidy analýza MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- RNA genetika izolace a purifikace MeSH
- separace buněk metody MeSH
- Check Tag
- lidé MeSH
Adult pancreatic stem and progenitor cells could represent an alternative source of insulin-producing tissue for diabetes treatment. In order to identify these cells, we have focused on the human pancreatic cells expressing cell surface molecule CD133, a marker of adult stem cells. We found that population of human CD133-positive pancreatic cells contains endocrine progenitors expressing neurogenin-3 and cells expressing human telomerase, ABCG2, Oct-3/4, Nanog, and Rex-1, markers of pluripotent stem cells. These cells were able to differentiate into insulin-producing cells in vitro and secreted C-peptide in a glucose-dependent manner. Based on our results, we suppose that the CD133 molecule represents another cell surface marker suitable for identification and isolation of pancreatic endocrine progenitors.
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- $a Laboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
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- $a Adult pancreatic stem and progenitor cells could represent an alternative source of insulin-producing tissue for diabetes treatment. In order to identify these cells, we have focused on the human pancreatic cells expressing cell surface molecule CD133, a marker of adult stem cells. We found that population of human CD133-positive pancreatic cells contains endocrine progenitors expressing neurogenin-3 and cells expressing human telomerase, ABCG2, Oct-3/4, Nanog, and Rex-1, markers of pluripotent stem cells. These cells were able to differentiate into insulin-producing cells in vitro and secreted C-peptide in a glucose-dependent manner. Based on our results, we suppose that the CD133 molecule represents another cell surface marker suitable for identification and isolation of pancreatic endocrine progenitors.
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