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In vitro neutralization of equid herpesvirus 1 mediated by recombinant antibodies
D Molinkova, P Skladal, V Celer
Journal of immunological methods.
2008 ;
333 (1-2) :
186-191.
Jazyk angličtina Země Nizozemsko
Perzistentní odkaz
https://www.medvik.cz/link/bmc11003860
Online
NLK
ScienceDirect (archiv)
od 1993-01-01 do 2009-12-31
- MeSH
- buněčné linie MeSH
- financování organizované MeSH
- fluorescenční mikroskopie MeSH
- herpesvirus 1 koní imunologie MeSH
- herpetické infekce imunologie veterinární MeSH
- imunoblotting MeSH
- imunoglobuliny - fragmenty imunologie MeSH
- kinetika MeSH
- koně MeSH
- molekulární sekvence - údaje MeSH
- nemoci koní virologie MeSH
- peptidová knihovna MeSH
- plakové testy MeSH
- proteiny virového obalu imunologie MeSH
- rekombinantní proteiny imunologie MeSH
- sekvence aminokyselin MeSH
- variabilní oblast imunoglobulinu imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
A phage antibody display library of single chain fragment variables (scFv) was applied to develop anti-equid herpesvirus-1 (EHV-1) glycoprotein D (gD) neutralizing antibodies. To enrich for specific scFvs, the phage antibody library was panned against epitope derived from the N-terminal part of EHV-1 gD. Unique clones were differentiated by BstNI fingerprinting and further characterized by sequencing and immunoreactivity. The neutralizing effect of each clone was assessed by plaque reduction assay. Three clones with neutralizing effect were isolated.
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