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In vitro neutralization of equid herpesvirus 1 mediated by recombinant antibodies
D Molinkova, P Skladal, V Celer
Journal of immunological methods.
2008 ;
333 (1-2) :
186-191.
Language English Country Netherlands
Persistent link
https://www.medvik.cz/link/bmc11003860
Online
NLK
ScienceDirect (archiv)
from 1993-01-01 to 2009-12-31
- MeSH
- Cell Line MeSH
- Financing, Organized MeSH
- Microscopy, Fluorescence MeSH
- Herpesvirus 1, Equid immunology MeSH
- Herpesviridae Infections immunology veterinary MeSH
- Immunoblotting MeSH
- Immunoglobulin Fragments immunology MeSH
- Kinetics MeSH
- Horses MeSH
- Molecular Sequence Data MeSH
- Horse Diseases virology MeSH
- Peptide Library MeSH
- Viral Plaque Assay MeSH
- Viral Envelope Proteins immunology MeSH
- Recombinant Proteins immunology MeSH
- Amino Acid Sequence MeSH
- Immunoglobulin Variable Region immunology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
A phage antibody display library of single chain fragment variables (scFv) was applied to develop anti-equid herpesvirus-1 (EHV-1) glycoprotein D (gD) neutralizing antibodies. To enrich for specific scFvs, the phage antibody library was panned against epitope derived from the N-terminal part of EHV-1 gD. Unique clones were differentiated by BstNI fingerprinting and further characterized by sequencing and immunoreactivity. The neutralizing effect of each clone was assessed by plaque reduction assay. Three clones with neutralizing effect were isolated.
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- $a A phage antibody display library of single chain fragment variables (scFv) was applied to develop anti-equid herpesvirus-1 (EHV-1) glycoprotein D (gD) neutralizing antibodies. To enrich for specific scFvs, the phage antibody library was panned against epitope derived from the N-terminal part of EHV-1 gD. Unique clones were differentiated by BstNI fingerprinting and further characterized by sequencing and immunoreactivity. The neutralizing effect of each clone was assessed by plaque reduction assay. Three clones with neutralizing effect were isolated.
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