-
Je něco špatně v tomto záznamu ?
Ultrastructural and nuclear antigen preservation after high-pressure freezing/freeze-substitution and low-temperature LR White embedding of HeLa cells
V Stradalova, K Gaplovska-Kysela, P Hozak
Jazyk angličtina Země Německo
NLK
SpringerLink Journals
od 2005-01-01 do 2008-12-31
ProQuest Central
od 1997-01-01 do 2017-12-31
Medline Complete (EBSCOhost)
od 2000-01-01 do Před 1 rokem
Nursing & Allied Health Database (ProQuest)
od 1997-01-01 do 2017-12-31
Health & Medicine (ProQuest)
od 1997-01-01 do 2017-12-31
Public Health Database (ProQuest)
od 1997-01-01 do 2017-12-31
- MeSH
- akrylové pryskyřice MeSH
- antigeny jaderné analýza MeSH
- buněčné jádro imunologie ultrastruktura MeSH
- financování organizované MeSH
- HeLa buňky MeSH
- imunohistochemie MeSH
- kryoprezervace metody MeSH
- lidé MeSH
- mrazová substituce MeSH
- tlak MeSH
- transmisní elektronová mikroskopie MeSH
- zalévání tkání plastickou hmotou metody MeSH
- Check Tag
- lidé MeSH
A protocol for high-pressure freezing and LR White embedding of mammalian cells suitable for fine ultrastructural studies in combination with immunogold labelling is presented. HeLa S3 cells enclosed in low-temperature gelling agarose were high-pressure frozen, freeze-substituted in acetone, and embedded in LR White at 0 degrees C. The morphology of such cells and the preservation of nuclear antigens were excellent in comparison with chemically fixed cells embedded in the same resin. The immunolabelling signal for different nuclear antigens was 4-to-13 times higher in high-pressure frozen than in chemically fixed cells. We conclude that one can successfully use high-pressure freezing/freeze-substitution and LR White embedding as an alternative of Lowicryl resins.
Citace poskytuje Crossref.org
- 000
- 02300naa 2200373 a 4500
- 001
- bmc11005103
- 003
- CZ-PrNML
- 005
- 20121031110457.0
- 008
- 110311s2008 gw e eng||
- 009
- AR
- 024 __
- $a 10.1007/s00418-008-0504-x $2 doi
- 035 __
- $a (PubMed)18797913
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Strádalová, Vendula $7 xx0142675
- 245 10
- $a Ultrastructural and nuclear antigen preservation after high-pressure freezing/freeze-substitution and low-temperature LR White embedding of HeLa cells / $c V Stradalova, K Gaplovska-Kysela, P Hozak
- 314 __
- $a Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, 142 20, Prague, Czech Republic.
- 520 9_
- $a A protocol for high-pressure freezing and LR White embedding of mammalian cells suitable for fine ultrastructural studies in combination with immunogold labelling is presented. HeLa S3 cells enclosed in low-temperature gelling agarose were high-pressure frozen, freeze-substituted in acetone, and embedded in LR White at 0 degrees C. The morphology of such cells and the preservation of nuclear antigens were excellent in comparison with chemically fixed cells embedded in the same resin. The immunolabelling signal for different nuclear antigens was 4-to-13 times higher in high-pressure frozen than in chemically fixed cells. We conclude that one can successfully use high-pressure freezing/freeze-substitution and LR White embedding as an alternative of Lowicryl resins.
- 650 _2
- $a akrylové pryskyřice $7 D000180
- 650 _2
- $a antigeny jaderné $x analýza $7 D034961
- 650 _2
- $a buněčné jádro $x imunologie $x ultrastruktura $7 D002467
- 650 _2
- $a kryoprezervace $x metody $7 D015925
- 650 _2
- $a mrazová substituce $7 D017110
- 650 _2
- $a HeLa buňky $7 D006367
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a imunohistochemie $7 D007150
- 650 _2
- $a transmisní elektronová mikroskopie $7 D046529
- 650 _2
- $a zalévání tkání plastickou hmotou $x metody $7 D016613
- 650 _2
- $a tlak $7 D011312
- 650 _2
- $a financování organizované $7 D005381
- 700 1_
- $a Kyselá, Katarína $7 xx0085314
- 700 1_
- $a Hozák, Pavel, $d 1958- $7 js20040517003
- 773 0_
- $t Histochemistry & Cell Biology $w MED00002042 $g Roč. 130, č. 5 (2008), s. 1047-1052 $x 0948-6143
- 910 __
- $a ABA008 $b x $y 1
- 990 __
- $a 20110414094530 $b ABA008
- 991 __
- $a 20121031110500 $b ABA008
- 999 __
- $a ok $b bmc $g 832950 $s 697153
- BAS __
- $a 3
- BMC __
- $a 2008 $b 130 $c 5 $d 1047-1052 $i 0948-6143 $m Histochemistry and cell biology $n Histochem Cell Biol $x MED00002042
- LZP __
- $a 2011-4B/vtme
