-
Something wrong with this record ?
A mouse speciation gene encodes a meiotic histone H3 methyltransferase
O Mihola, Z Trachtulec, C Vlcek, JC Schimenti, J Forejt
Language English Country United States
- MeSH
- Epigenesis, Genetic MeSH
- Financing, Organized MeSH
- Histone-Lysine N-Methyltransferase genetics chemistry metabolism MeSH
- Histones metabolism MeSH
- Hybridization, Genetic MeSH
- Crosses, Genetic MeSH
- Chromosome Mapping MeSH
- Meiosis MeSH
- Methylation MeSH
- Molecular Sequence Data MeSH
- Infertility, Male genetics MeSH
- Mice, Inbred C3H MeSH
- Mice, Transgenic MeSH
- Mice MeSH
- Ovary enzymology MeSH
- Gene Expression Regulation MeSH
- Amino Acid Sequence MeSH
- Testis enzymology MeSH
- Chromosomes, Artificial, Bacterial MeSH
- Genetic Speciation MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
Speciation genes restrict gene flow between the incipient species and related taxa. Three decades ago, we mapped a mammalian speciation gene, hybrid sterility 1 (Hst1), in the intersubspecific hybrids of house mouse. Here, we identify this gene as Prdm9, encoding a histone H3 lysine 4 trimethyltransferase. We rescued infertility in male hybrids with bacterial artificial chromosomes carrying Prdm9 from a strain with the "fertility" Hst1(f) allele. Sterile hybrids display down-regulated microrchidia 2B (Morc2b) and fail to compartmentalize gammaH2AX into the pachynema sex (XY) body. These defects, seen also in Prdm9-null mutants, are rescued by the Prdm9 transgene. Identification of a vertebrate hybrid sterility gene reveals a role for epigenetics in speciation and opens a window to a hybrid sterility gene network.
- 000
- 02719naa 2200541 a 4500
- 001
- bmc11006110
- 003
- CZ-PrNML
- 005
- 20121114100855.0
- 008
- 110331s2009 xxu e eng||
- 009
- AR
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Mihola, Ondřej $7 xx0140378
- 245 12
- $a A mouse speciation gene encodes a meiotic histone H3 methyltransferase / $c O Mihola, Z Trachtulec, C Vlcek, JC Schimenti, J Forejt
- 314 __
- $a Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague, Czech Republic.
- 520 9_
- $a Speciation genes restrict gene flow between the incipient species and related taxa. Three decades ago, we mapped a mammalian speciation gene, hybrid sterility 1 (Hst1), in the intersubspecific hybrids of house mouse. Here, we identify this gene as Prdm9, encoding a histone H3 lysine 4 trimethyltransferase. We rescued infertility in male hybrids with bacterial artificial chromosomes carrying Prdm9 from a strain with the "fertility" Hst1(f) allele. Sterile hybrids display down-regulated microrchidia 2B (Morc2b) and fail to compartmentalize gammaH2AX into the pachynema sex (XY) body. These defects, seen also in Prdm9-null mutants, are rescued by the Prdm9 transgene. Identification of a vertebrate hybrid sterility gene reveals a role for epigenetics in speciation and opens a window to a hybrid sterility gene network.
- 650 _2
- $a sekvence aminokyselin $7 D000595
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a mapování chromozomů $7 D002874
- 650 _2
- $a umělé bakteriální chromozomy $7 D022202
- 650 _2
- $a křížení genetické $7 D003433
- 650 _2
- $a epigeneze genetická $7 D044127
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a regulace genové exprese $7 D005786
- 650 _2
- $a vznik druhů (genetika) $7 D049810
- 650 _2
- $a histonlysin-N-methyltransferasa $x genetika $x chemie $x metabolismus $7 D011495
- 650 _2
- $a histony $x metabolismus $7 D006657
- 650 _2
- $a hybridizace genetická $7 D006824
- 650 _2
- $a mužská infertilita $x genetika $7 D007248
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a meióza $7 D008540
- 650 _2
- $a metylace $7 D008745
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a myši inbrední C3H $7 D008809
- 650 _2
- $a myši transgenní $7 D008822
- 650 _2
- $a molekulární sekvence - údaje $7 D008969
- 650 _2
- $a ovarium $x enzymologie $7 D010053
- 650 _2
- $a testis $x enzymologie $7 D013737
- 650 _2
- $a financování organizované $7 D005381
- 700 1_
- $a Trachtulec, Zdeněk $7 xx0140375
- 700 1_
- $a Vlček, Čestmír $7 xx0122524
- 700 1_
- $a Schimenti, J. C.
- 700 1_
- $a Forejt, Jiří, $d 1944- $7 jn20000400707
- 773 0_
- $t Science $w MED00004282 $g Roč. 323, č. 5912 (2009), s. 373-375
- 910 __
- $a ABA008 $b x $y 2
- 990 __
- $a 20110414112710 $b ABA008
- 991 __
- $a 20121114100910 $b ABA008
- 999 __
- $a ok $b bmc $g 833728 $s 698200
- BAS __
- $a 3
- BMC __
- $a 2009 $b 323 $c 5912 $d 373-375 $m Science $n Science $x MED00004282
- LZP __
- $a 2011-1B09/dkme
