• Something wrong with this record ?

Influence of collagen and chondroitin sulfate (CS) coatings on poly-(lactide-co-glycolide) (PLGA) on MG 63 osteoblast-like cells

M. Vandrovcová, T. Douglas, D. Hauk, B. Grössner-Schreiber, J. Wiltfang, L. Bačáková, P. H. Warnk

. 2011 ; 60 (5) : 797-813.

Language English Country Czech Republic

Document type Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc12006479

Poly-(lactide-co-glycolide) (PLGA) is an FDA-approved biodegradable polymer which has been widely used as a scaffold for tissue engineering applications. Collagen has been used as a coating material for bone contact materials, but relatively little interest has focused on biomimetic coating of PLGA with extracellular matrix components such as collagen and the glycosaminoglycan chondroitin sulfate (CS). In this study, PLGA films were coated with collagen type I or collagen I with CS (collagen I/CS) to investigate the effect of CS on the behaviour of the osteoblastic cell line MG 63. Collagen I/CS coatings promoted a significant increase in cell number after 3 days (in comparison to PLGA) and after 7 days (in comparison to PLGA and collagencoated PLGA). No influence of collagen I or collagen I/CS coatings on the spreading area after 1 day of culture was observed. However, the cells on collagen I/CS formed numerous filopodia and displayed well developed vinculin-containing focal adhesion plaques. Moreover, these cells contained a significantly higher concentration of osteocalcin, measured per mg of protein, than the cells on the pure collagen coating. Thus, it can be concluded that collagen I/CS coatings promote MG 63 cell proliferation, improve cell adhesion and enhance osteogenic cell differentiation.

References provided by Crossref.org

Bibliography, etc.

Literatura

000      
00000naa a2200000 a 4500
001      
bmc12006479
003      
CZ-PrNML
005      
20120420115629.0
007      
ta
008      
120301s2011 xr d f 000 0eng||
009      
AR
024    7_
$a 10.33549/physiolres.931994 $2 doi
040    __
$a ABA008 $d ABA008 $e AACR2 $b cze
041    0_
$a eng
044    __
$a xr
100    1_
$a Vandrovcová, Marta. $7 _AN051206 $u Institute of Physiology, Academy of Sciences of the Czech Republic, Prague
245    10
$a Influence of collagen and chondroitin sulfate (CS) coatings on poly-(lactide-co-glycolide) (PLGA) on MG 63 osteoblast-like cells / $c M. Vandrovcová, T. Douglas, D. Hauk, B. Grössner-Schreiber, J. Wiltfang, L. Bačáková, P. H. Warnk
504    __
$a Literatura $b 67
520    9_
$a Poly-(lactide-co-glycolide) (PLGA) is an FDA-approved biodegradable polymer which has been widely used as a scaffold for tissue engineering applications. Collagen has been used as a coating material for bone contact materials, but relatively little interest has focused on biomimetic coating of PLGA with extracellular matrix components such as collagen and the glycosaminoglycan chondroitin sulfate (CS). In this study, PLGA films were coated with collagen type I or collagen I with CS (collagen I/CS) to investigate the effect of CS on the behaviour of the osteoblastic cell line MG 63. Collagen I/CS coatings promoted a significant increase in cell number after 3 days (in comparison to PLGA) and after 7 days (in comparison to PLGA and collagencoated PLGA). No influence of collagen I or collagen I/CS coatings on the spreading area after 1 day of culture was observed. However, the cells on collagen I/CS formed numerous filopodia and displayed well developed vinculin-containing focal adhesion plaques. Moreover, these cells contained a significantly higher concentration of osteocalcin, measured per mg of protein, than the cells on the pure collagen coating. Thus, it can be concluded that collagen I/CS coatings promote MG 63 cell proliferation, improve cell adhesion and enhance osteogenic cell differentiation.
650    _2
$a buněčné inženýrství $x metody $7 D060846
650    _2
$a buněčné linie $7 D002460
650    _2
$a proliferace buněk $x účinky léků $7 D049109
650    _2
$a viabilita buněk $x účinky léků $7 D002470
650    _2
$a kultivované buňky $7 D002478
650    _2
$a chondroitinsulfáty $x farmakologie $x chemie $7 D002809
650    _2
$a biokompatibilní potahované materiály $x farmakologie $x chemie $7 D020099
650    _2
$a kolagen typu I $x farmakologie $x chemie $7 D024042
650    _2
$a lidé $7 D006801
650    _2
$a kyselina mléčná $x chemie $7 D019344
650    _2
$a testování materiálů $7 D008422
650    _2
$a osteoblasty $x cytologie $x fyziologie $x účinky léků $7 D010006
650    _2
$a osteogeneze $x fyziologie $x účinky léků $7 D010012
650    _2
$a kyselina polyglykolová $x chemie $7 D011100
650    _2
$a tkáňové podpůrné struktury $7 D054457
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Douglas, T. $u Department of Biomaterials, Radboud University Nijmegen Medical Center, Nijmegen, Netherlands
700    1_
$a Hauk, D. $u Department of Oral and Maxillofacial Surgery, University of Kiel, Kiel, Germany
700    1_
$a Grössner-Schreiber, B. $u Department of Operative Dentistry and Peridontology, School of Dentistry, University of Kiel, Kiel, Germany
700    1_
$a Wiltfang, J. $u Department of Oral and Maxillofacial Surgery, University of Kiel, Kiel, Germany
700    1_
$a Bačáková, Lucie, $d 1958- $7 xx0070525 $u Institute of Physiology, Academy of Sciences of the Czech Republic, Prague
700    1_
$a Warnke, P. H. $u Department of Oral and Maxillofacial Surgery, University of Kiel, Kiel, Germany; Faculty of Health Sciences and Medicine, Bond University, Gold Coast, Queensland, Australia
773    0_
$t Physiological research $x 0862-8408 $g Roč. 60, č. 5 (2011), s. 797-813 $w MED00003824
856    41
$u http://www.biomed.cas.cz/physiolres/archive.htm
910    __
$a ABA008 $b A 4120 $c 266 $y 2
990    __
$a 20120229072848 $b ABA008
991    __
$a 20120420115607 $b ABA008
999    __
$a ok $b bmc $g 899537 $s 763310
BAS    __
$a 3
BMC    __
$a 2011 $b 60 $c 5 $d 797-813 $i 0862-8408 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
LZP    __
$a 2012-12/ipme

Find record

Citation metrics

Archiving options