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Direct ESI-MS analysis of O-acyl glycosylated cardiolipins from the thermophilic bacterium Alicyclobacillus acidoterrestris
T Rezanka, L Siristova, K Melzoch, K Sigler
Jazyk angličtina Země Irsko
Typ dokumentu práce podpořená grantem
- MeSH
- Bacteria chemie MeSH
- glykosylace MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací metody MeSH
- hmotnostní spektrometrie metody MeSH
- izotopy kyslíku chemie MeSH
- kardiolipiny analýza izolace a purifikace MeSH
- Publikační typ
- práce podpořená grantem MeSH
We used direct ESI-MS analysis to identify derivatives of cardiolipin molecular species (i.e. O-acyl glycosylated cardiolipins) from the thermophilic bacterium Alicyclobacillus acidoterrestris. We used triple-quadrupole type mass spectrometer for analysis of this complex lipid and enzymatic hydrolysis and 1H and 13C NMR for the identification of these cardiolipin derivatives. These techniques enabled us to identify and quantify the specific molecular species profiles of derivatives of cardiolipin directly from lipid extracts of the bacterium including the identification of the sugar moiety as alpha-D-mannose and all five acyls including their positional isomers.
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- $a Řezanka, Tomáš $7 xx0106317 $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague, Czech Republic. rezanka@biomed.cas.cz
- 245 10
- $a Direct ESI-MS analysis of O-acyl glycosylated cardiolipins from the thermophilic bacterium Alicyclobacillus acidoterrestris $c T Rezanka, L Siristova, K Melzoch, K Sigler
- 520 9_
- $a We used direct ESI-MS analysis to identify derivatives of cardiolipin molecular species (i.e. O-acyl glycosylated cardiolipins) from the thermophilic bacterium Alicyclobacillus acidoterrestris. We used triple-quadrupole type mass spectrometer for analysis of this complex lipid and enzymatic hydrolysis and 1H and 13C NMR for the identification of these cardiolipin derivatives. These techniques enabled us to identify and quantify the specific molecular species profiles of derivatives of cardiolipin directly from lipid extracts of the bacterium including the identification of the sugar moiety as alpha-D-mannose and all five acyls including their positional isomers.
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- $a Bacteria $x chemie $7 D001419
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- $a kardiolipiny $x analýza $x izolace a purifikace $7 D002308
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- $a Clinical Laboratory Techniques
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- $a glykosylace $7 D006031
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- $a hmotnostní spektrometrie $x metody $7 D013058
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- $a izotopy kyslíku $x chemie $7 D010103
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- $a hmotnostní spektrometrie s elektrosprejovou ionizací $x metody $7 D021241
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- $a Siříšťová, Lucie. $7 _AN067744
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- $t Chemistry and physics of lipids $p Chem Phys Lipids $g Roč. 161, č. 2 (2009), s. 115-121 $w MED00002119 $x 0009-3084
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