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Insect cold tolerance and repair of chill-injury at fluctuating thermal regimes: role of 70 kDa heat shock protein expression
M Tollarova-Borovanska, L Lalouette, V Kostal
Language English Country Great Britain
Document type Research Support, Non-U.S. Gov't
- MeSH
- Adaptation, Physiological physiology MeSH
- Insecta physiology MeSH
- RNA, Messenger metabolism MeSH
- Cold Temperature MeSH
- HSP70 Heat-Shock Proteins physiology genetics MeSH
- Body Temperature Regulation physiology MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
Expression of heat shock proteins has been proposed as an underlying mechanism of increased cold tolerance in insects exposed to fluctuating thermal regimes (FTRs) in comparison to constant low temperatures (CLTs). We found that the levels of Pahsp70 mRNA increase by up to 3 orders in the linden bugs, Pyrrhocoris apterus exposed to FTR -5 degree C (22h) and 25 degree C (2h). The 2h-long warm pulses, however, were not sufficient for accumulation of PaHSP70 protein and thus no significant difference in expression of PaHSP70 protein was detected between FTR and CLT regimes. Hence, we conclude that the accumulation of PaHSP70 protein is not the mechanism underlying the increased cold tolerance in P. apterus at the particular FTR used in this study. The relevance of some other possible mechanisms is discussed.
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- $a Expression of heat shock proteins has been proposed as an underlying mechanism of increased cold tolerance in insects exposed to fluctuating thermal regimes (FTRs) in comparison to constant low temperatures (CLTs). We found that the levels of Pahsp70 mRNA increase by up to 3 orders in the linden bugs, Pyrrhocoris apterus exposed to FTR -5 degree C (22h) and 25 degree C (2h). The 2h-long warm pulses, however, were not sufficient for accumulation of PaHSP70 protein and thus no significant difference in expression of PaHSP70 protein was detected between FTR and CLT regimes. Hence, we conclude that the accumulation of PaHSP70 protein is not the mechanism underlying the increased cold tolerance in P. apterus at the particular FTR used in this study. The relevance of some other possible mechanisms is discussed.
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