Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Enhanced expressions of mRNA for neuropeptide Y and interleukin 1 beta in hypothalamic arcuate nuclei during adjuvant arthritis-induced anorexia in Lewis rats

A. Stofkova, M. Haluzik, B. Zelezna, A. Kiss, M. Skurlova, Z. Lacinova, J. Jurcovicova

. 2009 ; 16 (6) : 377-384.

Language English Country Switzerland

Document type Research Support, Non-U.S. Gov't

E-resources Online

NLK Karger Journals from 1994 to 2009

OBJECTIVE: Food intake is activated by hypothalamic orexigenic neuropeptide Y (NPY), which is mainly under the dual control of leptin and ghrelin. Rat adjuvant arthritis (AA), similarly as human rheumatoid arthritis, is associated with cachexia caused by yet unknown mechanisms. The aim of our study was to evaluate NPY expression in hypothalamic arcuate nuclei (nARC) under the conditions of AA-induced changes in leptin, ghrelin and adiponectin. Since IL-1beta is involved in the central induction of anorexia, we studied its expression in the nARC as well. METHODS: AA was induced to Lewis rats using complete Freund's adjuvant. On days 12, 15 and 18 after complete Freund's adjuvant injection, the levels of leptin, adiponectin, ghrelin and IL-1beta were determined by RIA or ELISA. The mRNA expressions for NPY, leptin receptor (OB-R), ghrelin receptor (Ghsr) and IL-1beta were determined by TaqMan RT-PCR from isolated nARC. RESULTS: In AA rats, decreased appetite, body mass and epididymal fat stores positively correlated with reduced circulating and epididymal fat leptin and adiponectin. Ghrelin plasma levels were increased. In nARC, mRNA for OB-R, Ghsr and NPY were overexpressed in AA rats. AA rats showed overexpression of mRNA for IL-1beta in nARC while circulating, and spleen IL-1beta was unaltered. CONCLUSION: During AA, overexpression of orexigenic NPY mRNA in nARC along with enhanced plasma ghrelin and lowered leptin levels occur. Decreased food intake indicates a predominant effect of the anorexigenic pathway. Activated expression of IL-1beta in nARC suggests its role in keeping AA-induced anorexia in progress. The reduction in adiponectin may also contribute to AA-induced anorexia. Copyright 2009 S. Karger AG, Basel.

000      
04135naa a2200637 a 4500
001      
bmc12009113
003      
CZ-PrNML
005      
20130726111934.0
008      
120319s2009 sz eng||
009      
AR
040    __
$d ABA008 $a ABA008 $b cze
041    0_
$a eng
044    __
$a sz
100    1_
$a Štofková, Andrea. $7 xx0246901 $u Department of Normal, Pathological, and Clinical Physiology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
245    10
$a Enhanced expressions of mRNA for neuropeptide Y and interleukin 1 beta in hypothalamic arcuate nuclei during adjuvant arthritis-induced anorexia in Lewis rats / $c A. Stofkova, M. Haluzik, B. Zelezna, A. Kiss, M. Skurlova, Z. Lacinova, J. Jurcovicova
520    9_
$a OBJECTIVE: Food intake is activated by hypothalamic orexigenic neuropeptide Y (NPY), which is mainly under the dual control of leptin and ghrelin. Rat adjuvant arthritis (AA), similarly as human rheumatoid arthritis, is associated with cachexia caused by yet unknown mechanisms. The aim of our study was to evaluate NPY expression in hypothalamic arcuate nuclei (nARC) under the conditions of AA-induced changes in leptin, ghrelin and adiponectin. Since IL-1beta is involved in the central induction of anorexia, we studied its expression in the nARC as well. METHODS: AA was induced to Lewis rats using complete Freund's adjuvant. On days 12, 15 and 18 after complete Freund's adjuvant injection, the levels of leptin, adiponectin, ghrelin and IL-1beta were determined by RIA or ELISA. The mRNA expressions for NPY, leptin receptor (OB-R), ghrelin receptor (Ghsr) and IL-1beta were determined by TaqMan RT-PCR from isolated nARC. RESULTS: In AA rats, decreased appetite, body mass and epididymal fat stores positively correlated with reduced circulating and epididymal fat leptin and adiponectin. Ghrelin plasma levels were increased. In nARC, mRNA for OB-R, Ghsr and NPY were overexpressed in AA rats. AA rats showed overexpression of mRNA for IL-1beta in nARC while circulating, and spleen IL-1beta was unaltered. CONCLUSION: During AA, overexpression of orexigenic NPY mRNA in nARC along with enhanced plasma ghrelin and lowered leptin levels occur. Decreased food intake indicates a predominant effect of the anorexigenic pathway. Activated expression of IL-1beta in nARC suggests its role in keeping AA-induced anorexia in progress. The reduction in adiponectin may also contribute to AA-induced anorexia. Copyright 2009 S. Karger AG, Basel.
590    __
$a bohemika - dle Pubmed
650    02
$a adiponektin $x metabolismus $7 D052242
650    02
$a zvířata $7 D000818
650    02
$a nechutenství $x genetika $x metabolismus $x patofyziologie $7 D000855
650    02
$a chuť k jídlu $x fyziologie $7 D001066
650    02
$a regulace chuti k jídlu $x fyziologie $7 D001069
650    02
$a nucleus arcuatus hypothalami $x metabolismus $x sekrece $7 D001111
650    02
$a artritida $x chemicky indukované $x komplikace $7 D001168
650    02
$a modely nemocí na zvířatech $7 D004195
650    02
$a regulace genové exprese $x fyziologie $7 D005786
650    02
$a ghrelin $x krev $x sekrece $7 D054439
650    02
$a systém hypotalamus-hypofýza $x metabolismus $x sekrece $7 D007030
650    02
$a interleukin-1beta $x genetika $7 D053583
650    02
$a leptin $x krev $x sekrece $7 D020738
650    02
$a mužské pohlaví $7 D008297
650    02
$a neuropeptid Y $x genetika $7 D009478
650    02
$a messenger RNA $x metabolismus $7 D012333
650    02
$a krysa rodu Rattus $7 D051381
650    02
$a potkani inbrední LEW $7 D011917
650    02
$a signální transdukce $x fyziologie $7 D015398
650    02
$a upregulace $x fyziologie $7 D015854
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Haluzík, Martin, $d 1970- $7 xx0000707
700    1_
$a Železná, Blanka. $7 xx0206931
700    1#
$a Kiss, Alexander. $7 _AN038558
700    1_
$a Škurlová, Martina. $7 xx0226211
700    1_
$a Lacinová, Zdeňka $7 xx0062138
700    1_
$a Jurčovičová, Jana $7 xx0103077
773    0_
$t Neuroimmunomodulation $p Neuroimmunomodulation $g Roč. 16, č. 6 (2009), s. 377-384 $x def $w MED00003488
773    0_
$p Neuroimmunomodulation $g 16(6):377-84, 2009
910    __
$a ABA008 $b x $y 4
990    __
$a 20120319150904 $b ABA008
991    __
$a 20130726112427 $b ABA008
999    __
$a ok $b bmc $g 902337 $s 766019
BAS    __
$a 3
BMC    __
$a 2009 $b 16 $c 6 $d 377-384 $i def $m Neuroimmunomodulation $x MED00003488
LZP    __
$a 2012-1Q10/

Find record

Citation metrics

Loading data ...

Archiving options

Loading data ...