• Je něco špatně v tomto záznamu ?

Anti-proliferative and anti-angiogenic effects of CB2R agonist (JWH-133) in non-small lung cancer cells (A549) and human umbilical vein endothelial cells: an in vitro investigation

B. Vidinský, P. Gál, M. Pilátová, Z. Vidová, P. Solár, L. Varinská, L. Ivanová, J. Mojžiš

. 2012 ; 58 (2) : 75-80.

Jazyk angličtina Země Česko

Typ dokumentu práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc12016245

Non-small cell lung cancer has one of the highest mortality rates among cancer-suffering patients. It is well known that the unwanted psychotropic effects of cannabinoids (CBs) are mediated via the CB1 receptor (R), and selective targeting of the CB2R would thus avoid side effects in cancer treatment. Therefore, the aim of our study was to evaluate the effect of selective CB2R agonist, JWH-133, on A549 cells (non-small lung cancer) and human umbilical vein endothelial cells (HUVECs). Cytotoxicity assay and DNA fragmentation assay were employed to evaluate the influence of JWH-133 (3-(1,1-dimethylbutyl)- 1-deoxy-Δ8-tetrahydrocannabinol) on investigated cancer cells. In addition, migration assay and gelatinase zymography were performed in HUVECs to asses JWH-133 anti-angiogenic activity. Our study showed that JWH-133 exerted cytotoxic effect only at the highest concentration used (10-4 mol/l), while inhibition of colony formation was also detected at the non-toxic concentrations (10-5–10-8 mol/l). JWH-133 was also found to be able to induce weak DNA fragmentation in A549 cells. Furthermore, JWH-133 at non-toxic concentrations inhibited some steps in the process of angiogenesis. It significantly inhibited endothelial cell migration after 17 h of incubation at concentrations of 10-4–10-6 mol/l. In addition, JWH-133 inhibited MMP-2 secretion as assessed by gelatinase zymography. The present study demonstrates the in vitro anti-proliferative and anti-angiogenic potential of CB2R agonist, JWH-133, in nonsmall lung cancer cells and HUVECs. Our results generate a rationale for further in vivo efficacy studies with this compound in preclinical cancer models.

Bibliografie atd.

Literatura

000      
00000naa a2200000 a 4500
001      
bmc12016245
003      
CZ-PrNML
005      
20130122104441.0
007      
ta
008      
120522s2012 xr df f 000 0eng||
009      
AR
040    __
$a ABA008 $d ABA008 $e AACR2 $b cze
041    0_
$a eng
044    __
$a xr
100    1_
$a Vidinský, B. $7 _AN067736 $u Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic
245    10
$a Anti-proliferative and anti-angiogenic effects of CB2R agonist (JWH-133) in non-small lung cancer cells (A549) and human umbilical vein endothelial cells: an in vitro investigation / $c B. Vidinský, P. Gál, M. Pilátová, Z. Vidová, P. Solár, L. Varinská, L. Ivanová, J. Mojžiš
504    __
$a Literatura $b 26
520    9_
$a Non-small cell lung cancer has one of the highest mortality rates among cancer-suffering patients. It is well known that the unwanted psychotropic effects of cannabinoids (CBs) are mediated via the CB1 receptor (R), and selective targeting of the CB2R would thus avoid side effects in cancer treatment. Therefore, the aim of our study was to evaluate the effect of selective CB2R agonist, JWH-133, on A549 cells (non-small lung cancer) and human umbilical vein endothelial cells (HUVECs). Cytotoxicity assay and DNA fragmentation assay were employed to evaluate the influence of JWH-133 (3-(1,1-dimethylbutyl)- 1-deoxy-Δ8-tetrahydrocannabinol) on investigated cancer cells. In addition, migration assay and gelatinase zymography were performed in HUVECs to asses JWH-133 anti-angiogenic activity. Our study showed that JWH-133 exerted cytotoxic effect only at the highest concentration used (10-4 mol/l), while inhibition of colony formation was also detected at the non-toxic concentrations (10-5–10-8 mol/l). JWH-133 was also found to be able to induce weak DNA fragmentation in A549 cells. Furthermore, JWH-133 at non-toxic concentrations inhibited some steps in the process of angiogenesis. It significantly inhibited endothelial cell migration after 17 h of incubation at concentrations of 10-4–10-6 mol/l. In addition, JWH-133 inhibited MMP-2 secretion as assessed by gelatinase zymography. The present study demonstrates the in vitro anti-proliferative and anti-angiogenic potential of CB2R agonist, JWH-133, in nonsmall lung cancer cells and HUVECs. Our results generate a rationale for further in vivo efficacy studies with this compound in preclinical cancer models.
650    02
$a kanabinoidy $x farmakologie $x metabolismus $7 D002186
650    02
$a nemalobuněčný karcinom plic $x farmakoterapie $7 D002289
650    02
$a buněčné kultury $7 D018929
650    02
$a nádorové buněčné linie $7 D045744
650    02
$a pohyb buněk $7 D002465
650    02
$a proliferace buněk $7 D049109
650    02
$a viabilita buněk $7 D002470
650    02
$a fragmentace DNA $7 D053938
650    02
$a endoteliální buňky pupečníkové žíly (lidské) $x účinky léků $7 D061307
650    02
$a lidé $7 D006801
650    02
$a nádory plic $x farmakoterapie $7 D008175
650    02
$a patologická angiogeneze $7 D009389
650    02
$a receptor kanabinoidní CB2 $x agonisté $x metabolismus $7 D043885
650    02
$a časové faktory $7 D013997
650    02
$a hojení ran $7 D014945
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Gál, Peter $7 xx0126576 $u Department of Pathological Anatomy, University of Veterinary Medicine and Pharmacy, Košice; East-Slovak Institute of Cardiovascular Diseases, Department for Biomedical Research, Košice; Institute of Histology and Embryology, 1st Faculty of Medicine, Charles University in Prague
700    1_
$a Pilátová, Martina $7 xx0169602 $u Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic
700    1_
$a Vidová, Z. $7 _AN067737 $u Department of Pharmacology, Pavol Jozef Šafárik University, Košice; 1st Department of Psychiatry, Louis Pasteur University Hospital and Pavol Jozef Šafárik University, Košice
700    1_
$a Solár, Peter, $d 1970- $7 xx0140463 $u Institute of Biology and Ecology, Pavol Jozef Šafárik University, Košice
700    1_
$a Varinská, Lenka. $7 _AN030341 $u Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic
700    1_
$a Ivanová, L. $7 _AN067738 $u Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic
700    1_
$a Mojžíš, Ján. $7 _AN036587 $u Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic
773    0_
$t Folia biologica $x 0015-5500 $g Roč. 58, č. 2 (2012), s. 75-80 $w MED00011004
856    41
$u https://fb.cuni.cz/file/5639/FB2012A0012.pdf $y plný text volně přístupný
910    __
$a ABA008 $b A 970 $c 89 $y 3
990    __
$a 20120521123510 $b ABA008
991    __
$a 20130122104558 $b ABA008
999    __
$a ok $b bmc $g 910019 $s 773376
BAS    __
$a 3
BMC    __
$a 2012 $b 58 $c 2 $d 75-80 $i 0015-5500 $m Folia biologica (Praha) $n Folia biol. (Praha) $x MED00011004
LZP    __
$a 2012-25/dkme

Najít záznam

Citační ukazatele

    Možnosti archivace