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Control of the interferon response in RNAi experiments
J. Nejepinska, M. Flemr, P. Svoboda,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- DNA Primers MeSH
- RNA, Double-Stranded MeSH
- HEK293 Cells MeSH
- HeLa Cells MeSH
- Interferons metabolism MeSH
- Cloning, Molecular MeSH
- Humans MeSH
- RNA, Small Interfering genetics MeSH
- Molecular Sequence Data MeSH
- Gene Expression Regulation MeSH
- RNA Interference MeSH
- Amino Acid Sequence MeSH
- Gene Silencing MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The RNA interference (RNAi) and interferons have been an uneasy marriage. Ever since the discovery of RNAi in mammals, the interferon response has been a feared problem. While RNAi became an efficient and widespread method for gene silencing in mammals, numerous studies recognized several obstacles, including undesirable activation of the interferon response, which need to be overcome to achieve a specific and robust RNAi effect. The aim of this text is to provide theoretical and practical information for scientists who want to control interferon response and other adverse effects in their RNAi experiments.
References provided by Crossref.org
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